Propriétés électrophysiologiques des canaux ioniques formés par la toxine nématicide Cry5Ba du bacille de Thuringe dans les bicouches lipidiques planes

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(4) . Abstract &U\WR[LQVDUHSURWHLQVV\QWKHWL]HGDVFU\VWDOLQFOXVLRQVE\WKHBacillus thuringiensis EDFWHULXPXSRQVSRUXODWLRQ7KH\DUHXVHGZLGHO\DVELRORJLFDOFRQWURODJHQWVDVWKH\H[KLELW WR[LFLW\WRDUDQJHRILQYHUWHEUDWHVLQFOXGLQJQHPDWRGHV7KH&U\%WR[LQVDUHDFWLYHDJDLQVW D QXPEHU RI SDUDVLWLF QHPDWRGH VSHFLHV VXFK DV Ancylostoma ceylanicum D KXPDQ JDVWUR LQWHVWLQDOSDUDVLWH6RIDUWKHPRGHRI DFWLRQRI QHPDWLFLGDO&U\WR[LQVLVODUJHO\XQNQRZQ H[FHSWIRUWKHIDFWVWKDWWKHLUVSHFLILFUHFHSWRUVDUHJO\FROLSLGVDQGWKDWWKH\FDXVHSURPLQHQW GDPDJH WR QHPDWRGH LQWHVWLQDO FHOOV ,Q WKLV VWXG\ ZH VKRZ IRU WKH ILUVW WLPH WKDW WKH QHPDWLFLGDO &U\%D WR[LQ D PHPEHU RI WKH WKUHH GRPDLQ IDPLO\ RI WR[LQV SURGXFHG E\ WKH Bacillus thuringiensisIRUPVSRUHVLQUHFHSWRUIUHHSODQDUOLSLGELOD\HUV7KHSRUHVIRUPHGE\ WKH WR[LQ ZHUH FDWLRQ VHOHFWLYH ERWK XQGHU DFLG DQG DONDOLQH S+ FRQGLWLRQV 8QGHU V\PPHWULFDO P0 .&O FRQGLWLRQV SRUH DFWLYLW\ ZDV FKDUDFWHUL]HG E\ FRQGXFWDQFHV UDQJLQJIURPWRS6DWERWKS+DQG7KHPRVWIUHTXHQWO\REVHUYHGFRQGXFWDQFH OHYHOVGLIIHUHGIURPHDFKRWKHUE\DSSUR[LPDWHO\WRS6FRQVLVWHQWZLWKWKHH[LVWHQFHRI FOXVWHUV RI GLIIHUHQW QXPEHU RI HOHPHQWDU\ VLPLODU FRRSHUDWLYHO\ JDWHG SRUHV RU ZLWK WKH SUHVHQFHRIYDULDEOHVL]HROLJRPHUVZLWKGLIIHUHQWSRUHGLDPHWHUV Keywords Bacillus thuringiensis QHPDWLFLGDO įHQGRWR[LQH &U\%D OLSLG ELOD\HU PHPEUDQHSRUHIRUPDWLRQHOHFWURSK\VLRORJ\KRPRORJ\PRGHOLQJC. elegans. LY.

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