• Aucun résultat trouvé

Extracellular vesicles secretion by Mycoplasma mycoides subsp. mycoides, the etiological agent of contagious bovine pleuropneumonia

N/A
N/A
Info
Télécharger
Protected

Academic year: 2021

Partager "Extracellular vesicles secretion by Mycoplasma mycoides subsp. mycoides, the etiological agent of contagious bovine pleuropneumonia"

Copied!
2
0
0

Chargement.... (Voir le texte intégral maintenant)

Texte intégral

(1)

Title: Extracellular vesicles secretion by Mycoplasma mycoides subsp. mycoides, the etiological agent of contagious bovine pleuropneumonia

Authors

Sarah Ganter1, Alexandre Villard1, Lucia Manso-Silvan2, Didier Chevret3, Christelle Boulé4, Véronique Monnet3, Florence Tardy1 and Patrice Gaurivaud1.

Institution

1 Université de Lyon, Anses, Laboratoire de Lyon, VetAgro Sup, UMR

Mycoplasmoses des Ruminants, 31 avenue Tony Garnier 69364 Lyon cedex 07, France

2 CIRAD-INRA, UMR 1309 ASTRE, F-34398 Montpellier, France

3 PAPPSO, Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

4 Université Claude Bernard Lyon 1, Centre Technologique des Microstructures, Service « Etudes à façon » EZUS Lyon, Villeurbanne, France

Extracellular vesicles (EV) are nano-sized, membrane-derived, non-self-replicating, spherical structures shed into the extracellular environment by both eukaryotic and prokaryotic cells. In bacteria, EV carry virulence factors like adhesins, toxins and immunomodulatory molecules. They are of importance for the bacteria –host interplay and hence vaccine development. Within the Mycoplasma (M.) genus EV have only been described, briefly, in M. gallisepticum so far. The aim of this study was to investigate the potential secretion of EV by M. mycoides subsp. mycoides (Mmm), the etiological agent of contagious bovine pleuropneumonia, and to characterize their membrane protein content.

Several culture conditions were tested to produce EV of Mmm strain Afadé in vitro. Vesicles were collected using a classical ultracentrifugation method and observed by

(2)

transmission electron microscopy. Different approaches, such as gradient density and cultures in minimal medium were tested in order to improve EV purification. EV membrane proteins were extracted with triton x-114 and identified by mass spectrometry.

Mmm was shown to release EV in vitro, their size and concentration being dependent

on growth conditions. Mmm vesicles were produced by living cells and not by cells with decreased viability after stresses. Their release happened in a budding-way from the cell surface as observed by electron miscroscopy. Several virulence factors were identified in EV membranes suggesting that EV could be involved in host-mycoplasma interactions.

Mmm can produce in vitro extracellular vesicles that carry virulence factors. Whether

Références

Télécharger maintenant ( PDF - 2 Page - 73.96 KB )

Documents relatifs

Molecular typing of Mycoplasma mycoides subspecies mycoides Small Colony strains by SSAP

In the case of MmmSC, it has been previously shown that strains may be differentiated by the Southern blot technique using IS1296 or IS1634 as labelled probes.. Each allele

Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia

As shown in Figure 1, an increase in the percentages of CD4 + T cells and the prolifera- tion (i.e., growth index above 2) was detected but only in stimulated cultures prepared

Genotyping of Mycoplasma mycoides subsp. mycoides SC by multilocus sequence analysis allows molecular epidemiology of contagious bovine pleuropneumonia

The use of a technique based on sequencing multiple loci, designated multilocus sequence analysis (MLSA), allowed the identification of 15 dif- ferent allelic profiles within

Antigenic and genetic characterisation of lipoprotein lppC from Mycoplasma mycoides subsp. mycoides SC

mycoides SC strains tested showed the expected reacting band for the functional lppC gene at 3.1 kb, corresponding to the part cloned in plasmid pJFFL2IA, as well as a band at 1.5

Etude de la synthèse des exopolysaccharides sécrétés par les mycoplasmes du groupe "mycoides" et, notamment, par Mycoplasma mycoides subsp. mycoides, agent de la péripneumonie contagieuse bovine

Enfin, la disponibilité de séquences génomiques complètes et annotées des espèces de mycoplasmes du groupe « mycoides » nous a fourni la possibilité d’identifier les gènes

Characterization of Free Exopolysaccharides Secreted by Mycoplasma mycoides Subsp. mycoides

We finally conducted in silico analyses of Mmm genes potentially involved in polysaccharide biosynthesis and proposed candidate pathways that might account for the

Study of Mycoplasma mycoides subsp. Mycoides exopolysaccharides

The aim of this study was to develop a suitable methodology to produce and purify Mmm EPS, to measure their carbohydrate content and to compare them biochemically to the

Evolutionary history of contagious bovine pleuropneumonia using next generation sequencing of mycoplasma mycoides subsp. mycoides "small colony"

robust phylogeny for this pathogen, establish when the most recent common ancestor of all MmmSC strains appeared, determine whether CBPP existed in Africa or, more likely, it