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Contribution to the study of semiochemical slow release formulations. Development of flash chromatographic methods

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Academic year: 2021

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Contribution to the study of semiochemical

Contribution to the study of semiochemical

slow release formulations

slow release formulations

Development of flash chromatographic methods

Stéphanie Heuskin

Department of Analytical chemistry (Prof. G. Lognay)

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Introduction

Introduction

Semiochemicals = communication signals between species (pheromones, kairomones, allomones, synomones)

Aphids tritrophic system

Host plant (Vicia fabae L.) Phytophageous insect : aphids Predator : Episyrphus balteatus De Geer Parasitoid : Aphidius ervi Haliday E-β-farnesene Alarm pheromone

Aggregation of Harmonia axyridis

Larva of Asian lady beetle Adult eating aphids Aggregated colony β-caryophyllene Aggregation pheromone

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Goals of the research

Goals of the research



Isolation of semiochemicals from essential

oils



Formulations of compounds to attract predators

and/or parasitoids of aphids on infested fields



Chemical ecology supported by analytical

chemistry methodologies

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Steps

Steps

Pure essential oils

Flash Chromatography fractionation Purified semiochemicals Evaporation of elution solvent Solvent-free semiochemicals Alginate beads formulations Slow release study Biological tests GCMS analysis + Fast GC analysis Fast GC analysis Fast GC analysis Optimization

+ Fast GC method validation by accuracy profiles and analytical performance estimation

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For more details, please see the

For more details, please see the

poster

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