TRACE ELEMENT KINETICS IN
CONTAMINATED POSIDONIA
OCEANICA MEADOW
J. Richir , N. Luy , G. Lepoint, A. Alvera Azcarate, R. Biondo, S. Gobert
Essaouira 05-2012
Mediterranean submitted to anthropic pressures
Introduction
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bioindicator : Posidonia oceanica - monitoring of coastal ecosystem pollution;
- representative accumulation; - pollutant biologically accessible. pollution trace elements (TEs)
TE Cd Pb Cu Zn Cr Fe Ni
# ref. 39 30 28 26 23 15 14
TE As Ag Co Mn Al Bi V Mo
# ref. 4 3 3 3 2 1 1 1
Mediterranean submitted to anthropic pressures
Introduction
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bioindicators : Posidonia oceanicapollution trace elements (TEs)
TEs uptake and loss kinetics of P.
oceanica contaminated in their
natural environment?
Mediterranean submitted to anthropic pressures
Introduction
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bioindicators : Posidonia oceanicapollution trace elements (TEs)
TEs uptake and loss kinetics of P.
oceanica contaminated in their
natural environment?
Objectives:
To study TEs kinetics and tissue compartmentalization of P.
ocenica contaminated in situ at 2 experimental levels:
- moderate: 21x Calvi SW conc.; 4x world ocean mean conc. - acute: 151x Calvi SW conc.; 31x world ocean mean conc.
In situ experimental contaminations
M A T E R I A L -M E T H O D S
Contamination at moderate levels
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5 days of contamination in June 2009;•
410L bell-shaped mesocosm;•
Contamination every 12 hours (9am-9pm);•
15 days of decontamination.M A T E R I A L -M E T H O D S
M A T E R I A L -M E T H O D S
Contamination at acute levels
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24h00 of contamination in June 2009;•
54L rectangular-box mesocosm;•
5 contaminations, 1 every 3 hours (9am-9pm);Posidonia oceanica
(A) shoot of leaves on a plagiotropic rhizome (Rz); (B, C) adult leaves (AL); (D) intermediate leaf (IL); (E) juvenile leaf.
Libes and Boudouresque,1987
plagiotropic shoot orthotropic shoot rhizome blade roots M A T E R I A L -M E T H O D S limb ligula
leaf base (petiole)
E D C B A root leaf base stipule scale rhizome
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C O N C L U S I O N
Conclusion
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P. oceanica takes up TEs from the beginning of experiments;•
Moderate contamination design are preferred;•
Once contaminations end, TE concentrations rapidly decrease;•
Leaves exhibited different uptake kinetics: the younger growing leaves forming new tissues incorporated chemicals more rapidly than the older senescent leaves;•
Rhizomes did not show any clear trend.P. oceanica is a sensitive sentinel to delineate
punctual pollutions similar to what might be measured in contaminated Mediterranean waters routine use in regularly scheduled monitoring programs.
Q U E S T I O N S
Thank you for
your attention
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Analytical steps: • homogeneous sample • acidic digestion in a microwave oven • measures : inductively coupled plasma mass spectrometer M A T E R I A L -M E T H O D S
