Retinoic X Receptor Alpha Heterodimer Activation by Selective VDR Modulator and VDRE Sequence
PEPTIDES: SEQUENCE ANALYSIS 505 - 518
ThP 505 LC-MS/MS – Peptide Mapping with Electron Transfer Dissociation (ETD) Reveals Enzyme-Mediated Intramolecular Bond in Transglutaminase; Thomas W Powers1; Andrew W Dawdy1; Jason C Rouse2; Olga V Friese1; 1Pfizer, Inc. Chesterfield, MO; 2Pfizer, Inc. Andover, ThP 506 MAHeavyweight Champion: Iodine’s Large Mass-Defect
Weighs in on the Enhancement of the Sequence Coverage of Tryptic Peptides via ETD; Emilie Aude Viglino1; Christopher Shaffer1; František Tureček1;
1University of Washington, Seattle, WA
ThP 507 Electron Transfer Dissociation of Trivalent Metal Cationized Acidic Phosphorylated Peptides; Juliette Joan Commodore1; Carolyn J. Cassady1; 1The University of Alabama, Tuscaloosa, AL
ThP 508 Comparative Study of The Skin peptidomes of Rana ridibunda from Russian and Slovenian Populations;
Tatiana Yu Samgina1; Konstantin A Artemenko2; Jonas Bergquist3; Polonca Trebse4; Gregor Torkar4; Maria Tolpina1; Albert T. Lebedev5; 1Moscow State University, Moscow, Russia; 2Uppsala University, Uppsala, SE; 3Uppsala University, Uppsala, Sweden; 4Ljubljana University, Ljubljana, Slovenia; 5Moscow State University, Moscow, Moscow
ThP 509 Sensitive and Rapid LC/MS Method to Identify Potential T-cell Epitopes of Antibody Drugs; Nobuo Sekiguchi1; Chiyomi Kubo1; Ayako Takahashi1; Kumiko Muraoka1; Akira Takeiri1; Mariko Yano1; Shotaro Takata1; Naoaki Murao1; Masaki Ishigai1; 1Chugai Pharmaceutical Co., Ltd., Gotemba, Shizuoka
ThP 510 Complete Sequence Coverage via ECD in a Q-ToF versus ETD/CID/HCD in an Orbitrap of 6+ and 7+
Protonated Ubiquitin; Yury V Vasil’ev1; Nathan I Lopez1; Valery G Voinov1; Douglas F Barofsky2; Joseph S Beckman1;
1Linus Pauling Institute, Oregon State University Corvallis, OR; 2Oregon State University, Department of Chemistry Corvallis, OR
ThP 511 Highly Specific Peptide-Bond Dissociation of Some Peptide Model Molecules; Chen-Lin Liu; NSRRC, Hsinchu, Hsinchu
ThP 512 De novo Sequencing of Novel Antimicrobial Peptides from the Venom of the Scorpion Isometrus maculatus using ASDF-incorporated Curved Field Reflectron.;
Yuzo Yamazaki1; Atsushi Kitanaka2; Mao Yakio2; Masahiro Miyashita3; Hisashi Miyagawa3; 1Shimadzu Corporation, Kyoto, Japan; 2Graduate School of Agriculture, Kyoto
GENERAL INFORMATION THURSDAY POSTERS
University Kyoto, Japan; 3Graduate School of Agriculture, Kyoto University, Kyoto, Japan
ThP 513 Compact Mass Spectrometry (CMS) for Reaction Optimization and Quality Control of Modern Solid Phase Synthesis (SPPS) of Biologically Active Peptides;
Simon J Prosser1; Daniel Eikel1; James P Cain2; Elizabeth Restituyo-Rosario2; 1Advion, Inc. Ithaca, NY; 2Protein Technologies Inc., Tucson, AZ
ThP 514 3-Hydroxy-4-nitrobenzoic Acid (3H4NBA) as a MALDI Matrix for In-Source Decay; Yuko Fukuyama1; Shunsuke Izumi2; Koichi Tanaka3; 1Shimadzu Corporation, Kyoto; 2Hiroshima University, Higashi-Hiroshima, Japan;
3Shimadzu Corporation, Kyoto, Japan
ThP 515 Application of Hydrogen Attachment/Abstraction Dissociation (HAD) for Peptide Analysis; Sadanori Sekiya1; Hidenori Takahashi1; Takashi Nishikaze1; Shosei Yamauchi1; Shinichi Iwamoto1; Koichi Tanaka1; 1Shimadzu Corporation, Kyoto, Japan
ThP 516 Selective Derivatization of Peptide Carboxyl Groups with Fluorophores for LDI-MS Analyses; Dilrukshika S.
W. Palagama1; Raymond E West III1; Dragan Isailovic1; 1The University of Toledo, Toledo, OH
ThP 517 Elucidating the Mechanism of Photo-Induced Peptide Cleavage by Uranyl using Mass Spectrometry;
Rasmus Elnegaard1; Niels Erik Møllegaard2; Thomas J D Jørgensen1; Frank Kjeldsen1; 1Department of Biochemistry and Molecular Biology, University of Sourthern Denmark, Odense, Denmark; 2Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark
ThP 518 Fragmentation of Model Deprotonated Phosphorylated Peptides by Collision-Induced Dissociation; Chelsea E Plummer1; Suma Kavati1; Junjie Gao1; Carolyn J Cassady1;
1The University of Alabama, Tuscaloosa, AL PEPTIDOMICS
519 - 530
ThP 519 Comparing Mass Spectrometric Platforms for
Neuropeptide and Neuroproteome Discovery from Well-Defined Neuronal Tissue; Krishna D.B Anapindi1; Elena V Romanova2, 3; Bruce R Southey2; Jonathan V Sweedler2;
1University of Illinois-Urbana Champaign, Urbana, IL;
2University of Illinois at Champaign, Urbana-Champaign, IL; 3Beckman Institute, UIUC Urbana, IL ThP 520 Correlating Differential Peptidomics and
Transcriptomics Data for Assessing Seasonal Changes in the Camel Brain; Elena Romanova1; Bruce R Southey1; Michael Greenwood2; Charles Hindmarch3; Yea-Ling Tay4; Ahmad Yamin bin Abdul Rahman4; Kok-Gan Chan5; Mark Rogers2; Colin Campbell2; F. Zahra Djazouli Alim6; David Murphy2; Jonathan V. Sweedler1; 1University of Illinois at Urbana-Champaign, Urbana-Champaign, IL; 2University of Bristol, Bristol, UK; 3Queen’s University, Kingston, Canada;
4BioEasy Sdn Bhd, Shah Alam, Selangor Darul Ehsan, Malaysia; 5University of Malaya, Kuala Lumpur, Malaysia;
6Université Saad Dahlab de Blida, Blida, Algeria ThP 521 Multifaceted Mass Spectrometric Investigation
of Neuropeptide Changes in Blue Crab, Callinectes sapidus, in Response to Ocean Acidification; Yang Liu1; Amanda Buchberger1; Lingjun Li1, 2; 1Department of Chemistry, University of Wisconsin, Madison, WI; 2School of Pharmacy, University of Wisconsin, Madison, WI
ThP 522 Mass Spectrometric Quantification and Imaging of Changes in Crustacean Neuropeptide Expression Levels Resulting from Hypoxia Stress; Kellen DeLaney1; Amanda Buchberger1; Yang Liu1; Lingjun Li1; 1UW Madison, Madison, WI
ThP 523 A Puzzle Approach on Single Cell Level; Susanne Neupert; Biocenter Cologne, University of Cologne 50674 Cologne, Germany
ThP 524 Large Scale Discovery and de novo-Assisted Sequencing of Native Cationic Antimicrobial Peptides (CAMPs) from the Komodo Dragon (Varanus
komodoensis).; Paul Russo1; Melanie Juba2; Megan Devine2; Stephanie Barksdale2; Kajal Gupta2; Shaylyn Scott2; Kent Vliet3; Joel Schnur2; Monique vanHoek2; Barney Bishop2; 1George Mason University, Manassas, VA; 2George Mason Universtiy, Manassas, VA; 3University of Florida, Gainesville, FL
ThP 525 Immunogenic HLA-DR-presented-peptides Identified from Clinical Samples of Synovial Tissue/Fluid and Peripheral Blood from Patients with Rheumatoid Arthritis or Lyme Arthritis; Qi Wang1; Elise E Drouin2; Chunxiang Yao1; Jiyang Zhang1; Yu Huang1; Deborah R Leon1; Allen C Steere2; Catherine E Costello1; 1Boston University School of Medicine, Boston, MA; 2Massachusetts General Hospital and Harvard Medical School, Boston, MA ThP 526 Proto-Peptidomics: A UPLC-Ion Mobility-MS/MS
Proteomics Workflow for Studying Early Peptide Evolution on the Prebiotic Earth; Jay G. Forsythe1, 2; Anton S. Petrov1, 2; Sheng-Sheng Yu1, 2; W. Calvin Millar1,
2; Martha A. Grover1, 2; Ramanarayanan Krishnamurthy2,
3; Nicholas V. Hud1, 2; Facundo M. Fernandez1, 2; 1Georgia Tech, Atlanta, GA; 2NSF/NASA Center for Chemical Evolution, Atlanta, GA; 3The Scripps Research Institute, La Jolla, CA
ThP 527 LC-MS-MS identification of Small Cyclic Amphipathic Peptides (SCAmpPs) in citrus phloem; Leslie Harden1; William H. Vensel2; Kent F. McCue2; William R. Belknap2;
1USDA/WRRC, Albany, CA; 2U.S. Dept. of Agriculture, Albany, CA
ThP 528 Azoniaspiro[4.4]nonyl Scaffold for Quantitative Peptide Analysis by LC-ESI-MS(/MS); Bartosz Setner1; Monika Kijewska1; Alicja Kluczyk1; Piotr Stefanowicz1; Zbigniew Szewczuk1; 1Faculty of Chemistry, University of Wrocław, Wrocław, Poland
ThP 529 Electroanalysis and Electrospray Ionization Mass Spectrometry of Intact and Oxidized Hormone Glucagon; Wendell P. Griffith1; Sushma Karra1; Robert Kennedy2; Waldemar Gorski1; 1University of Texas at San Antonio, San Antonio, TX; 2University of Michigan, Ann Arbor, MI
ThP 530 Proteomic Landscapes ofPachycondila Villosa Ant Venom by Nano-Scale Chromatography and High Resolution Mass Spectrometry; Camila Takeno Cologna1; Loic Quinton1; Edwin De Pauw1; Michel Degueldre1;
1University of Liege, GIGA-Proteomics Liege, Belgium PROTEIN THERAPEUTICS: STRUCTURAL
CHARACTERIzATION 531 - 566
ThP 531 Profiling Released High Mannose and Complex N-Glycan Structures from Monoclonal Antibodies Using Optimized Hydr; Scott McCall1; Matthew Lauber1; Stephan Koza1; Erin Chambers1; 1Waters, Milford, MA
ThP 532 Comprehensively Characterizing the N and O-Linked Glycosylation of a Recombinant Human EPO using HILIC-MS; Matthew Lauber1; Stephan Koza1; Erin Chambers1; 1Waters Corporation, Milford, MA ThP 533 Mass Spectrometry Friendly Method for N-glycan
Quantification; Wei Wu1; Jiping Zhou1; Subbarao Mantha1; Zhichao Fang1; Michelle Wang1; Li Tao1; Tapan Das2; Reb Russell2; 1Bristol-Myers Squibb Company, Bloomsbury, NJ;
2Bristol-Myers Squibb, Hopewell, NJ
ThP 534 Site-specific Characterization of N-/O- Glycosylation on Therapeutic Human Chorionic Gonadotropins using Online LC/MS and Tandem Mass Tags; Hongbin Zhu1; Ashley C Ruth1; David A Keire1; Hongping Ye1; 1FDA, St.
Louis, MO
THURSDAY POSTERS
ThP 535 Substrate- and Substrate-mimetic Chaperone Binding Sites in Human Alpha-Galactosidase Revealed by Affinity- Mass Spectrometry; Michael Przybylski1; Stefan Maeser2; Adrian Moise2; Frederike Eggers2; Mary Murphy3; Jeff Bornheim3; 1Steinbeis Centre Biopolymer Analysis and Biomedical Mass Spectrometry, Ruesselsheim am Main, Germany; 2Steinbeis Centre Biopolymer Analysis and Biomedical Mass Spectrometry, Rüsselsheim am Main, Germany; 3Reichert-Ametek Technologies, Buffalo, NY ThP 536 Hydrogen Exchange Mass Spectrometry can Reliably
Detect Small Fractions of Destabilized Protein in Comparability Studies; Tyler Hageman1; David D Weis1; Jayant Arora1; 1University of Kansas, Lawrence, KS ThP 537 Characterization of Bispecific Heteromab using LC-MS
Based Methods; Yuping Zhou1; Jason Tang1; 1Eli Lilly and Company, Indianapolis, IN
ThP 538 Disulfide Bond Mapping-A Case Study through Partial Reduction; Song Klapoetke; KBI, Durham, NC
ThP 539 Innovator and Biosimilar Infliximab: Comparability Assessment of the Host Cell Proteins and Protein Higher Order Structure; Jing Fang1; Catalin E Doneanu1; William R Alley1; Ying-Qing Yu1; Asish Chakraborty1; Alain Beck2; Weibin Chen1; 1Waters, Milford, MA; 2Centre d’Immunologie Pierre Fabre, Saint-Julien-en-Genevois, France
ThP 540 Protein Conformational Analysis of Native and Stressed Monoclonal Antibodies using Ion Mobility and LC/MS/
MS Disulfide Bond Mapping; David L Wong1; Staples O Gregory1; Jordy Hsiao1; Te-Wei Chu1; Stephen Madden1; Javier Satulovsky1; 1Agilent Technologies, Santa Clara, CA ThP 541 Mapping of Disulfide Bonds by Reducing
Electrochemistry and Mass Spectrometry; Christian Cramer1; Kim F. Haselmann1; Jesper V. Olsen2; Peter Kresten Nielsen1; 1Novo Nordisk A/S, Måløv, Denmark;
2NNF Center for Protein Research, University of Copenhagen, Denmark
ThP 542 Protein Dynamics in the TGF-β Superfamily by Hydrogen Deuterium Exchange Mass Spectrometry;
Roxana E. Iacob1; Viet Le2; Bo Zhao2; Yuan Tian2; Timothy A. Springer2; John R. Engen3; 1Northeastern University , Boston, MA; 2Children’s Hospital Boston and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA; 3Northeastern University, Boston, MA
ThP 543 Characterizing Deamidation and Oxidation in
Adalimumab with Low pH Peptide Mapping and Middle-Up Mass Spec Analysis; Chris Hosfield1; Eric Largy2; Anicet Catrain2; Fabrice Cantais2; Gery Van Vyncht2; Michael M Rosenblatt1; Sergei Saveliev1; Marjeta Urh1; Arnaud Delobel2; 1Promega Corporation, Madison, WI;
2Quality Assistance, Thuin, Belgium
ThP 544 Deciphering Biotherapeutic Protein Disulfide Bonds by Top and Middle-Down Mass Spectrometry; Jiang Zhang1; Pilsoo Kang1; Jianmei Kochling1; 1Sanofi Genzyme, Framingham, MA
ThP 545 Investigation of Antibody Aggregates by Hydrogen / Deuterium Exchange Mass Spectrometry and Other Biophysical Tools; Richard Yu-Cheng Huang1; Deqiang Yu2; Yuanli Song2; Ryan K. Swanson2; Zhijun Tan2; Elizabeth Schutsky2; Angela Lewandowski2; Zhengjian Li2; Guodong Chen1; 1Bristol-Myers Squibb, Princeton, NJ; 2Bristol-Myers Squibb, Devens, MA
ThP 546 Disulfide Bond Identification of Biotherapeutic Proteins using Various Fragmentation Techniques Available on an Orbitrap Fusion Tribrid Mass Spectrometer; Stephane Houel1; Jennifer Sutton1; Seema Sharma1; Terry Zhang1; Romain Huguet1; Martin Samonig2; Vlad Zabrouskov1; Jonathan Josephs1; 1Thermo Fisher Scientific, San Jose, CA; 2Thermo Fisher Scientific, Germering, Germany
ThP 547 Rapid Profiling of Stressed PEGylated Protein
Pharmaceuticals using Ion-Exchange Chromatography with Online Detection by Native ESI and Top-Down MS/MS; Khaja Muneeruddin1; Cedric E Bobst1; Ruth Frenkel2; Damian Houde2; Iva Turyan2; Zoran Sosic2; Igor A Kaltashov1; 1University of Massachusetts, Amherst, MA;
2Biogen, Cambridge, MA
ThP 548 A High-Throughput, Automated Platform Method for Multi-Attribute LC-MS Analysis of Cell Culture Harvest and Antibody Glycosylation; Jia Dong1; Nicole Migliore1; Steven J Mehrman1; John Cunningham1; Michael J Lewis1; Ping Hu1; 1Janssen Research & Development, Malvern, PA ThP 549 Automated Data Analysis Enabling Mass Spectrometry–
based Assays for the Developability Assessment of Biotherapeutics; Maurizio Bronzetti1; Cassandra Wigmore2; Joe Shambaugh1; David Bush1; Arnd Brandenburg2; Peter Haberl3; 1Genedata Inc, Lexington, MA; 2Genedata AG, Basel, Switzerland; 3Genedata GmbH, Munich, Germany ThP 550 Characterization of Selected Therapeutic Monoclonal
Antibodies under Stressed Conditions; Robert Carney1; Zsuzsa Lakos1; Elena Dremina1; John Snyder1; 1Eurofins Lancaster Laboratories, Inc., Lancaster, PA
ThP 551 Site-specific Glycoform Mapping of Biotherapeutics with Micro-Heterogeneity and Macro-Heterogeneity;
Youngsuk Seo1, 2; Myung Jin Oh1, 2; David Bradley3; Hyun Joo An1, 2; 1AGRS, Chungnam National University, Daejeon, Korea; 2GRAST, Chungnam National University, Daejeon, Korea; 3Agilent Technologies, Santa Clara, CA
ThP 552 Biosimilar Insulin Characterization: Top/Middle/Bottoms up Approaches; Rishikant Gupta1; Mithun Bhatt1; Annu Uppal2; Manoj Pillai2; Archana Krishnan1; Sanjay Sonar1;
1Biogenomics, Thane (w), Mumbai, India; 2SCIEX, 121 Udyog Vihar Phase IV Gurgaon, India
ThP 553 Mass Spectrometry Based Comparison of Rituximab Originator and Biosimilar; Othman Montacir1; Murat Eravci2; Andreas Springer3; Stephan Hinderlich4; Maria Kristina Parr1; 1Institute of Pharmaceutical and Medicinal Chemistry, Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, Germany., Berlin, Germany;
2Institut für Chemie und Biochemie, Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, Germany., Berlin, Germany; 3Großgerätezentrum BioSupraMol, Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, Germany., Berlin, Germany; 4Beuth Hochschule für Technik Berlin - University of Applied Sciences, Department of Life Sciences and Technology, Berlin, Germany., Berlin, Germany
ThP 554 Towards the Fast and Increasingly Simplified
Analysis of Trisulfide Formation in Biopharmaceutical Antibodies; Anja Resemann1; Rainer Paape1; Christoph Nordmann1; Waltraud Evers1; Lars Vorwerg1; Eckhard Belau1; Volker Sauerland1; Lisa A Marzilli2; Jason C Rouse2; Jason S Wood3; Detlev Suckau1; 1Bruker Daltonic GmbH, Bremen, Germany; 2Biotherapeutics Pharm. Sci., Pfizer Inc.
Andover, MO; 3Bruker Daltonic, Billerica, MA ThP 555 Site-Specific Characterization and Quantitation of
Protein Pegylation by LC-UV-MS and Standard Addition Approach; Yanjun Liu1; Shiaw-Lin Wu1, 2; William Hancock1;
1Barnett Inst., Northeastern University Boston, MA;
2BioAnalytix Inc, Cambridge, MA
ThP 556 High Resolution Separations for Protein LC/MS; Barry Boyes1; Benjamin Libert2; Stephanie E Schuster2; Brian E Wagner2; William E Miles2; Joseph Kirkland2; 1Advanced Materials Technology Inc, Wilmington, DE; 2Advanced Materials Technology Inc, Wilmington, DE
ThP 557 Complete Characterization of a Cysteine-linked Antibody-Drug Conjugate Performed on a Hybrid Quadrupole-Orbitrap Mass Spectrometer with High Mass Range; Aaron Oakley Bailey1; Eugen Damoc2; Kai
GENERAL INFORMATION THURSDAY POSTERS
Scheffler2; Stephane Houel1; Jonathan L Josephs1; 1Thermo Fisher Scientific, San Jose, CA; 2Thermo Fisher Scientific, Bremen, DE
ThP 558 Characterization of Therapeutic Protein Charge Heterogeneity by Isoelectric Focusing and Nano-spray LC-MS; Janet Lau1; Chen Li1; Shiaw-Lin (Billy) Wu1;
1BioAnalytix Inc, Cambridge, MA
ThP 559 Separation and Characterization of IgG2 Disulfide Isoforms by pH Gradient Cation Exchange
Chromatography and Non-Reduced LC/MS - Peptide Mapping; Kristin J Boggio1; Anastasiya P Manuilov1; Thomas J Porter1; Jason C Rouse1; Tanya Q. Shang1; Matthew C Thompson1; 1Pfizer Inc, Andover, MA ThP 560 High Throughput Screening and Characterization
of Bispecifics Using Native Ion Mobility Mass Spectrometry; Caroline S. Chu1; Te-Wei Chu1; Gregory O Staples1; Patrick D Perkins1; Andy Gieschen2; Christian Klein1; Carol H. Ball3; Ning Tang1; 1Agilent Technologies, Santa Clara, CA; 2Agilent Technologies, La Jolla, CA;
3Agilent Technologies, Wilmington, DE
ThP 561 MS-Based Epitope Mapping of Malaria Antigens: DBP and CelTOS from Plasmodium vivax and Plasmodium falciparum; Yining Huang1; Manolo D Plasencia1; Henry W Rohrs1; Nicole D Salinas2; Edwin Chen2; Niraj H Tolia2; Michael L Gross1; 1Washington University in St. Louis, Saint Louis, MO; 2Washington University School of Medicine, Saint Louis, MO
ThP 562 A Workflow to Identify Mutations of Bulk Recombinant Proteins by using N-terminal Labeling Combined with the MELD Method; Gabriel Mazzucchelli1; Denis Morsa1; Dominique Baiwir1; Nicolas Smargiasso1; Edwin De Pauw1;
1Univeristy of Liege, MS Lab - GIGA, Liege, Belgium ThP 563 Higher Order Structure of Monoclonal Antibodies
using Covalent Labeling Techniques and Integrative Biophysics; Mark Chance1; Parminder Kaur2; Janna G Kiselar1; 1Case Western Reserve University, Cleveland, OH;
2NeoProteomics, Inc. Cleveland, OH ThP 564 On-line Disulfide Cleavage for Structural
Characterization; J.C. Yves Leblanc1; Tanya Gamble2;
1SCIEX, Concord, ON; 2SCIEX, Concord ON, Canada ThP 565 Biopharmaceutical Erythropoeitin Characterisation:
Critical Quality Attribute (CQA) Mapping using LC-ESI-Qtof and Automated Database Searching of Glycopeptide Analytes; Daniel Ian Richard Spencer1; Julia Smith2; Richard Andrew Gardner1; Anja Resemann3; Peter Hufnagel3; Daryl Ludger Fernandes1; 1Ludger Ltd., Oxford, UK; 2Bruker Ltd. Coventry, Coventry, UK; 3Bruker Daltonic GmbH, Bremen, Germany
ThP 566 An Investigation into Aβ/Metal Binding via MALDI TOF MS; Andrea Renee Kelley1; George Perry1; Stephan B.H.
Bach1; 1University of Texas at San Antonio, San Antonio, TX PROTEINS: CONFORMATION ANALYSIS AND
STRUCTURAL BIOLOGY