Set up all Monday posters ...7:30 – 8:00 am Odd-numbered posters present ...10:30 am – 1:00 pm Even-numbered posters present ...12:00 – 2:30 pm Remove all Monday posters ...7:30 – 8:00 pm Antibodies & Antibody Drug Conjugates (Intact and
Characterization) ...001 - 020 Biomolecular Structure Analysis: Covalent Labeling
and Related Software ...021 - 039 Carbohydrates ...040 - 055 Data Independent Acquisition ...056 - 070 Diagnostic Clinical Chemistry (Applications) ...071 - 094 Drug and Metabolite Analysis: Novel Approaches for
Dried Biological Samples ...095 - 103 Energy: Hydrocarbon and Petrochemical (Ultra Hi Res) ...104 - 122 Environmental Analysis: General (Part 1)...123 - 147 Environmental Analysis: Pharmaceuticals and Pesticides ...148 - 176 Epigenetic Modifications ...177 - 194 High Mass Accuracy/High Performance MS Applications ...195 - 210 Imaging MS: Instrumentation...211 - 231 Imaging MS: Sample Preparation...232 - 243 Imaging MS: Software ...244 - 249 Informatics: Metabolomics ...250 - 264 Informatics: Multiomics Integration ...265 - 280 Instrumentation: New Concepts ...281 - 302 Intact Proteins...303 - 319 Ion Activation/Dissociation ...320 - 340 Ion Mobility: Applications (Other/Instrumentation) ...341 - 373 Ion Molecule, Ion/Ion, Ion/Electron Interactions ...374 - 398 Ion Spectroscopy ...399 - 411 LC-MS: Chromatography and Software (Part 1) ...412 - 440 Lipids: General ...441 - 456 Metabolomics: General...457 - 495 Microorganisms: Identification and Characterization ...496 - 517 Phosphopeptides: Enrichment Methods ...518 - 529 Polymers...530 - 548 Proteins: General and Membrane ...549 - 571 Proteins: PTMs (Part 1) ...572 - 596 Proteomics: Clinical Applications (Applied Proteomics) ...597 - 621 Proteomics: Quantitative ...622 - 650 Small Molecules: Qualitative Analysis ...651 - 680 Systems Biology (Multiomics and Other)...681 - 707 Top Down Protein Analysis (Applications) ...708 - 727 ANTIBODIES & ANTIBODY DRUG CONJUGATES (INTACT &
CHARACTERIzATION) 001 - 020
MP 001 Integrating Intact, Reduced and IdeS Approaches for Rapid Differentiation and Verification of Monoclonal Antibodies Using Liquid Chromatography Q-Exactive Mass Spectrometry; Lianji Jin; FDA, Cincinnati, OH MP 002 Online Separation and Characterization of intact IgG
Fabs Using Ultra Performance Liquid Chromatography Coupled with Top-down Mass Spectrometry; Zhe Wang1; Kenneth Smith2; Si Wu1; 1University of Oklahoma, Dept.
of Chem & Biochem Norman, OK; 2Oklahoma Medical Research Foundation, Oklahoma City, OK
MP 003 Evaluation of Different Immunocapture Platforms for Antibody-Conjugate Drug Quantification; Ling Xu1; Shaoxia Yu2; Jing-Tao Wu3; Mark Qian3; 1Millennium Pharma, Cambridge, MA; 2Takeda Pharmaceutical
International Inc., Cambridge, MA; 3Takeda Pharmaceuticals International Inc., Cambridge, MA
MP 004 Intact Mass Analysis of Antibody-drug conjugates (ADCs) as a Tool to Measure Payloads of Linked Cytotoxic Drugs; Joe Shambaugh1; Cassandra Wigmore2; Maurizio Bronzetti1; David Bush1; Arnd Brandenburg2; Peter Haberl3; 1Genedata Inc, Lexington, MA; 2Genedata AG, Basel, Switzerland; 3Genedata GmbH, Munich, Germany
MP 005 Characterization of Bispecific Antibodies Using Mass Spectrometry during Cell Line Development is Critical for an Optimized Final Drug Product; Michael Bacica1; Jon R Fitchett1; Stephen J Demarest1; Xiufeng Wu1; Robert Peery2; Bryan Jones1; 1Lilly Biotech Center-San Diego, San Diego, CA; 2Lilly BioTDR, Indianapolis, IN
MP 006 Developability Assessment of Multispecific Biopharmaceuticals; Kadir Ilker Sen1; Eric Beil1; Hirsh Nanda1; Andrew Mahan1; Darryl Davis1; 1Janssen Research and Development, Spring House, PA
MP 007 Monitoring the Critical Quality Attributes of Antibody Drug conjugates (ADCs) as Part of Biosimilar Development: Case Studies of ado-trastuzumab emtansine; Liuxi Chen1; Min Du1; Henry Y Shion1; Ying-Qing Yu1; Lan Wang2; Kai Gao2; Weibin Chen1; 1Waters Corporation, Milford, USA; 2National Institutes for food and drug control, Beijing, CN
MP 008 Detection of an Amino Acid Sequence Variant as a Function of Cell Line Stability in a Model Antibody;
Sylwia Jozwiak1; James Graham1; 1Lonza, Slough, UK MP 009 Assessment of Critical Parameters for the Mass
Spectrometric Evaluation of the Drug-to-Antibody Ratio in Antibody-Drug Conjugates; Neil Hershey1; Shiyue Zhou2; Yunping Huang1; Hangtian Song1; Hui Wei1; Jacob Bongers1; Richard Ludwig1; Li Tao1; Tapan Das1; 1 Bristol-Myers Squibb, Hopewell, NJ; 2Texas Tech University, Lubbock, Texas
MP 010 An Automated Quantitative Mass Spectrometry Assay to Determine Intra-Cellular Retention Kinetics of Antibiotic Released from Anti-Staphylococcus Aureus Antibody-Antibiotic Conjugate; Hilda Hernandez-Barry1; Kimberly Kajihara2; Daniel Tran2; Martine Darwish2; Byoung-Chul Lee2; Richard Vandlen2; Leanna Staben2; Thomas Pillow2; Wouter Hazenbos2; Kelly Loyet2; 1Genentech, South San Francisco, CA; 2Genentech Inc, South San Francisco, CA MP 011 An Automated Sample Extraction and Processing
Method Combining Ligand Binding and Mass
Spectrometry Analysis for Biotherapeutics Quantitation;
Ian Moore; SCIEX, Concord, ON
MP 012 A Comprehensive Mass Spectrometric Workflow for Characterizing Therapeutic Antibodies; Alicia Bielik1; Paula Magnelli1; Beth McLeod1; Colleen McClung1; Beth Paschal1; Cristian I Ruse1; Xiaofeng Shi1; Ellen Guthrie1;
1New Englanb Biolabs, Ipswich, MA
MP 013 Drug-to-Antibody Ratio Determination for Antibody-Drug Conjugates in Serum Enabled by a Sample Preparation Platform that Automates Affinity
Purification and Deglycosylation; Jing Chen1; Maryann Shen2; Steve Murphy1; 1Agilent Technologies, Inc. Madison, WI; 2Agilent Technologies, Santa Clara, CA
MP 014 Platform Method for Identity and Characterization of Therapeutic Monoclonal and Bispecific Antibodies by Intact and Reduced Mass Spectrometry; Richard Seipert1; Lisa Patterson2; George Tsui2; Chris Yu2; 1Genentech, South San Francisco, CA; 2Genentech Inc, South San Francisco, MP 015 CAA Tag-free Collisionally-Induced Fragmentation
Technique Applied to Studies of Antibody-drug Conjugate Lysosomal Processing; Andrew Bessire1; Chakrapani Subramanyam2; My-Hahn Lam3; Frank Loganzo3; T. Eric Ballard2; 1Pfizer, Inc., Groton, CT; 2Pfizer, Groton, CT; 3Pfizer Oncology-Rinat R&D, Pearl River, NY MP 016 Strategy to Rapidly and Comprehensively Characterize
Innovator Biologics and their Biosimilars via Stress Testing Studies; Esthelle Hoedt1; St John Skilton2; Yong J Kil3; Eric Carlson4; Chris Becker3; Beatrix M Ueberheide1;
1NYU School of Medicine, New York City, NY; 2Protein Metrics, Hopkinton, MA; 3Protein Metrics, San Carlos, CA;
4Protein Metrics Inc., San Carlos, CA
GENERAL INFORMATION
MP 017 Method Development for the Detection and Quantitation of Monomethyl Auristatin F (MMAF) in Cell Culture Media; Maria Christina Malinao1; Melissa M. Williams1; Cody Warhurst1; Julien Dugal-Tessier1; Josh T. Snyder1; Brian Mendelsohn1; Hui Zhao1; Sara Gulesserian1; Hosein Kouros-Mehr1; Kendall Morrison1; 1Agensys, Inc. Santa Monica, CA
MP 018 In-Depth Characterization of Monoclonal Antibodies with a Single Experiment and Fully Automated Data Analysis; Paul Taylor1; Johnathan Krieger1; Qixin Liu2; Mingjie Xie2; Lian Yang2, 3; Bin Ma3; 1Hospital for Sick Children, Toronto, Canada; 2Rapid Novor Inc., Waterloo ON, Canada; 3University of Waterloo, Waterloo ON, Canada
MP 019 Antibody Variable Domains as Intramolecular
Luciferase Inhibitors for the Generation of Proteolysis-Activated Biosensors; Lining Zhu1; Teresa Hong1; Nicola McNiven1; Markus Kalkum1; 1City of Hope, Duarte, CA MP 020 Novel Strategy Based on Dual Enrichment and Double
Lists for Characterization of Host Cell Proteins; Zheng-Xiang Zhang1; Jimmy Chan1; Shuai Zuo1; Tao Bo1; Rong An1; 1Agilent Technologies (China) Limited, Beijing, China BIOMOLECULAR STRUCTURE ANALYSIS: COVALENT
LABELING AND RELATED SOFTWARE 021 - 039
MP 021 Using High Resolution Structural Analysis to Determine if FPOP Modification Perturbs Native Protein Structure;
Emily Hart1; Lisa M Jones1; 1IUPUI Department of Chemistry & Chemical Biology, Indianapolis, IN MP 022 Extension of the FPOP Method for Oxidative
Modification in Live Tissue; Jessica Arlett Espino1; Anthony J Baucum1; Lisa M Jones2; 1Indiana University-Purdue University Indianapolis, Indianapolis, IN; 2Indiana University Purdue University Indianapolis, Indianapolis, In MP 023 Epitope Map of Malarial Antigen PvDBP revealed by
Fast Photochemical Oxidation of Proteins (FPOP);
Manolo David Plasencia1; Yining Huang1; Henry W Rohrs1; Edwin Chen2; Nichole D. Salinas2; Niraj H. Tolia2; Michael L Gross1; 1Washington University in St. Louis, St. Louis, MO;
2Washington University School of Medicine, St. Louis, MO MP 024 The Application of “Droplet-Like” Methods to FPOP;
Don L. Rempel1; Supratik Dutta2; Ben Niu2; Yining Huang2; Manolo Plasencia2; Michael Gross2; 1Center for Biomedical and Bioorganic Mass Spectrometry, Washington University in St. Louis, St Louis, MO; 2Center for Biomedical and Bioorganic Mass Spectrometry, Washington University in St. Louis, St. Louis, MO
MP 025 Normalization of High Resolution Hydroxyl Radical Protein Footprinting Data to Protein Structural Properties; Boer Xie1; Amika Sood1; Robert J Woods1; Joshua S. Sharp2; 1University of Georgia, Athens, GA;
2University of Mississippi, University, MS
MP 026 Automated Hydroxyl Radical Protein Footprinting for the Determination of Biopharmaceutical Protein Conformation; Franklin E. Leach III1; Ron Orlando2; Joshua S Sharp3; 1Photochem Technologies, Athens, GA;
2Complex Carbohydrate Research Center UGA, Athens, GA; 3School of Pharmacy, University of Mississippi, Oxford, MP 027 MSDevelop Isotope-encoded Photoaffinity Footprinting
Reagent for Structural Mass Spectrometry Studies;
Ming Cheng1; Michael L Gross1; 1Washington University in St. Louis, St. Louis, MO
MP 028 Exploring the Peroxidase Activation and Deactivation of Cytochrome C by Heme-Catalyzed Oxidative Labeling and ESI-MS; Victor Yin1; Lars Konermann1;
1Western University, London, Canada
MP 029 Comprehensive Measurement of Protein Dynamics in Complex Environments Using Footprinting Mass Spectrometry with Inexpensive MS2 Quantification of Labeled Cysteines; Jenna Gray Caldwell1; Pehr A. B.
Harbury1; 1Stanford University School of Medicine, Palo Alto MP 030 CAUsing Covalent Labeling and Mass Spectrometry
to Study b-2-Microglobulin-Inhibitor Binding Sites; Tianying Liu1; Richard W Vachet1; 1University of Massachusetts Amherst, Amherst, MA
MP 031 Towards an Improved Understanding of Diazirine Labeling for Structural Mass Spectrometry Applications; Daniel Ziemianowicz1; Chris Etienne2; David Schriemer1; 1University of Calgary, Calgary, Canada;
2Thermo Fisher Scientific, Rockford, IL
MP 032 Characterisation Proteolytic Process by Protein N-Terminal Derivatisation in- Gel Using MS-Based Approach in Drosophila; Nina Guillaumot1; Florian Veillard2; Jean-Marc Reichhart2; Alain Van dorsselaer1; Christine Schaeffer-Reiss1; 1Laboratoire de Spectrométrie de Masse BioOrganique, IPHC, Université de Strasbourg, CNRS, UMR7178, Strasbourg, Strasbourg, France; 2Institut de Biologie Moléculaire et Cellulaire, IBMC, Université de Strasbourg, CNRS UPR 9022, Strasbourg, Strasbourg, France
MP 033 Covalent Labeling Denaturation Mass Spectrometry for Sensitive Localized Higher Order Structure Comparisons; James A. Madsen1; Yan Yin1; Jing Qiao1; Vanessa Gill1; Kutralanathan Renganathan1; Wing-Yee Fu1; Stephen Smith1; James Anderson1; 1Momenta Pharmaceuticals, Cambridge, MA
MP 034 Xilmass: A New Approach Towards the Identification of Cross-Linked Peptides; Şule Yılmaz1, 2, 3; Friedel Drepper4,
5; Maša Černič6; Kris Gevaert1, 7; Anastassios Economou8,
9; Bettina Warscheid4, 5; Lennart Martens1, 7, 10; Elien Vandermarliere1, 7, 10; 1Department of Biochemistry, Ghent University, Ghent, BELGIUM; 2Medical Biotechnology Center, Ghent, BELGIUM; 3Bioinformatics Institute Ghent, Ghent, BELGIUM; 4Department of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology, University of Freiburg, Freiburg, Germany;
5BIOSS Centre for Biological Signaling Studies, University of Freiburg, Freiburg, Germany; 6Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Ljubljana, Slovenia; 7Medical Biotechnology Center, VIB, Ghent, BELGIUM; 8Laboratory of Molecular Bacteriology, Rega Institute for Medical Research, Department of Microbiology and Immunology, KULeuven, Leuven, BELGIUM; 9Institute of Molecular Biology and Biotechnology-FoRTH, Department of Biology – University of Crete, Iraklio, Crete, Greece; 10Bioinformatics Institute Ghent, Ghent University, Ghent, BELGIUM
MP 035 Crosslinkers with Isotope-Coding and Cleavable Spacers Improve Peptide Crosslink Identification by a Semi-Supervised Machine Learning Algorithm; Karl A T Makepeace1; Evgeniy V Petrotchenko1; Christoph H. Borchers2, 3; 1University of Victoria - Genome BC Proteomics Centre, Victoria, BC, Canada; 2University of Victoria - Genome BC Proteomics Centre, Victoria, BC;
3Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada
MP 036 A Simple Approach for Creating Protein Structural Models Based on Cross-Linking Data; Zsuzsanna Orban-Nemeth1; Evelyn Rampler1; Thomas Stranzl1; David Maria Hollenstein2; Otto Hudecz3; Peter Schloegelhofer4; Karl Mechtler1, 3; 1Institute of Molecular Pathology, Vienna, Austria; 2Department of Biochemistry and Cell Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria; 3Institute of Molecular Biotechnology, Austrian Academy of Sciences, Vienna, Austria; 4Department
MONDAY POSTERS
MONDAY POSTERS
of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria
MP 037 CX-Circos: A Web-Based Tool for Visualization and Analysis of Chemical Crosslinking Data; Junjie Wang1; Yi Shi1; Brian T. Chait1; 1Rockefeller University, New York, NY MP 038 In Cell Protein Footprinting Coupled with Mass
Spectrometry to Probe Protein Conformational Change;
Maissa M Gaye1; Lisa Jones1; 1Indiana University-Purdue University Indianapolis, Indianapolis, IN
MP 039 High-throughput Mass Spectrometric Analysis of Covalent Protein-inhibitor Adducts, Monoclonal Antibodies and ADCs: A Complete Workflow; Tisha San Miguel1; Jelly Netirojjanakul1; Rowe Todd1; Onea Daniel1; Victor Cee1; Arvedson Tara1; McCarter John1; Campuzano Iain1; 1Amgen, Inc., Thousand Oaks, CA
CARBOHYDRATES 040 - 055
MP 040 Quantifying Cyclodextrin-Drug Interactions by ESI-MS;
Emma-Dune Leriche1; Ping Zhang2; Aixia Wang2; Yuyu Yao1; Chang-Chun Ling2; Elena N Kitova1; John S Klassen1;
1University of Alberta, Edmonton, Canada; 2University of Calgary, Calgary, Canada
MP 041 Identification and Relative Quantification
C-mannosylation of Interleukin 6 (IL-6) using Liquid Chromatography-tandem Mass Spectrometry; Anhdao Darcy; Abbvie Bioresearch Center, Worcester, MA MP 042 Development of Negative Mode Free Radical Activated
Glycan Structure Elucidation Reagents; Jinshan Gao1; Nikunj Desai1; Jungeun Lee1; 1Montclair State University, Montcalir, NJ
MP 043 Carbohydrate Analysis using Gas Chromatography Vacuum Ultraviolet Spectroscopy in Comparison with Gas Chromatography Mass Spectrometry; Jamie Schenk1; Xiaojian Mao1; Jonathan Smuts2; Gabe Nagy3; Nicola L. B. Pohl3; Phillip Walsh2; Peter Kroll1; Kevin A Schug1; 1University of Texas at Arlington, Arlington, TX;
2VUV Analytics, Inc., Cedar Park, TX; 3Indiana University, Bloomington, IN
MP 044 Understanding the Reaction Products of Methanolysis and Butanolysis of Heparan Sulfate; Marten F Snel1; Paul J Trim2; John J Hopwood2; Qi Qi He3; Vito Ferro3; 1South Australian Health and Medical Research Institute, Adelaide, South Australia; 2LDRU, Nutrition and Metabolism Theme, South Australian Health and Medical Research Institute, Adelaide, Australia; 3School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia
MP 045 LC-MS Analysis of the Complete Molecular Weight Distribution of Low Molecular Weight Heparins; Yuewei Sheng1; Chun Shao1; Kshitij Khatri1; Joseph Zaia2; 1Boston University School of Medicine, Boston, MA; 2Boston University, Boston, MA
MP 046 Size-Selective Controlled Heparin Depolymerization to Obtain Heparin Oligosaccharides; Sandeep K Misra1; Joshua S Sharp1; 1Dept of Biomolecular Sciences, University of Mississippi, Oxford, MS
MP 047 Oxidation of Glucose at Gold Nanoparticles and Mass Spectrometric Product Analysis; Marilyn Wooten1; Wendell P. Griffith2; Sushma Karra2; Waldemar Gorski2;
1Trinity University, San Antonio, TX; 2University of Texas at San Antonio, San Antonio, TX
MP 048 Study of Rearrangement Products in Collision-Induced Dissociation and Surface-Induced Dissociation of Oligosaccharides and Glycoconjugates; Forouzan Aboufazeli1; Eric D Dodds1; 1University of Nebraska-Lincoln, Lincoln, NE
MP 049 Single Stage Tandem Mass Spectrometry Assignment of the C-5 Uronic Acid Stereochemistry in Heparan Sulfate Tetrasaccharides Using Electron Detachment Dissociation; Isaac Agyekum1; Chengli Zong2, 3; Geert-Jan Boons2, 3; Jon Amster2; 1University of Georgia,Chemistry Department, Athens, GA; 2University of Georgia, Chemistry Department Athens, GA; 3CCRC, University of Georgia Athens, GA
MP 050 Mass Spectrometric Quantification of Cell Wall Composition Differences in Enterococcus Using Stable Isotope Labeling by Amino Acids; James Chang1; Ashley Wallace1; Erin Foster1; Alex Guinn1; Sung Joon Kim1;
1Baylor University, Waco, TX
MP 051 High Resolution CESI-MS Analysis of APTS-Labeled N-Glycans of Biopharmaceutical Interest; Fonslow Bryan; Marton Szigeti1; Andras Guttman2; 1University of Debrecen, Debrecen, Hungary; 2AB Sciex, San Diego, CA MP 052 Probing Carbohydrate Isomerism through Transition
Metal Ion Adduction, Gas-Phase Ion Chemistry, and Collision-Induced Dissociation; Katherine Schumacher1; Yuting Huang1; Lauren Petrosh1; Eric D Dodds1; 1University of Nebraska - Lincoln, Lincoln, NE
MP 053 ESI-TOF-MS Study of Carbohydrate Interaction with Metal Complexes with Carboxylate-Rich Ligand; Fangzhi Yan1; Christopher D. Stewart1; Stephan B. H. Bach1; Wendell P Griffith1; Ghezai T. Musie1; 1University of Texas at San Antonio, San Antonio, TX
MP 054 Fragmentation of Derivatized and Non-Derivatized Metal-Adducted Oligosaccharides; Ranelle Schaller-Duke; The University of Alabama, Tuscaloosa, Al MP 055 A Novel Method for High-Throughput Analysis of
Bioactive Oligosaccharides; Randall Robinson1; Daniela Barile1; 1University of California, Davis, Davis, CA
DATA INDEPENDENT ACQUISITION 056 - 070
MP 056 Advances in Targeted Omics Quantitation Using a Novel Scanning Quadrupole DIA Method; Chris Hughes1; Keith Richardson1; Jason Wildgoose1; Martin R Green1; Richard Chapman1; Arkadiusz Grzyb1; Praveen Harapanahalli1; Kirsten Craven1; 1Waters, Wilmslow, United Kingdom
MP 057 Optimisation of Data Independent Acquisition (DIA) Data Extraction for Proteome Quantification; Gauthier Husson1; Alvaro Sebastian Vaca Jacome1; Maxime Eveque1; Christine Carapito1; 1Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), IPHC, Strasbourg University, CNRS, UMR7178, Strasbourg, France MP 058 Improved Multiplexed Data Independent Acquisition:
Sensitivity, Dynamic Range, and Full-Spectrum Demultiplexing; Jarrett Egertson1; Ying Sonia Ting1; Philip M Remes2; Romain Huguet2; Derek Bailey2; Michael Senko2; Vlad Zabrouskov2; Michael J MacCoss1; 1Univ of Washington, Seattle, WA; 2Thermo Fisher Scientific, San Jose, CA
MP 059 Analytical Validation of a Data-Independent Acquisition Mass Spectrometry Workflow for Quantification and Identification of Myofilament Proteoforms in Cardiac Biopsies; Irene van den Broek1; Irina Tchernyshyov1; Vidya Venkatraman1; Giulio Agnetti2, 3; Amol Prakash4; Scott Peterman5; Jennifer E Van Eyk1; 1Advanced Clinical Biosystems Research Institute, Heart Institute, Cedars Sinai Medical Center, Los Angeles, CA; 2Division of Cardiology. Johns Hopkins University School of Medicine, Baltimore, MD; 3Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy; 4Optys Tech Corporation, Brighton, MA; 5Thermo Fisher Scientific, Cambridge, MA