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4.4 Extraction topographique

4.4.5 Méthodes alternatives pour l’extraction topographique

Inicialmente o projeto de pesquisa iria englobar a medição de marcadores metabólicos, de estresse e de inflamação em resposta ao exercício aeróbico e neuromuscular agudos em mulheres entre 18 e 45 anos de idade portadoras da SM. Para execução deste projeto, no entanto, seria necessária a implantação de uma sala de exercícios físicos nas dependências do Hospital Universitário Onofre Lopes (HUOL). Embora tenha existido um grande esforço de pessoas como o Prof. Dr. George Dantas, de seu orientando na época Prof. Dr. Eduardo Caldas e da orientadora deste trabalho, a Dra. Maria Bernardete, não foi possível esta implantação. Esta situação gerou um obstáculo metodológico que impediu a execução do projeto inicial.

Paralelamente, como as atividades de revisão da literatura estavam em andamento, observou-se a oportunidade de preparar um artigo de revisão sistemática ( RS) nesta área que iria contribuir para sistematizar as bases teóricas do conhecimento. Este foi o artigo aceito para publicação. Devido à proximidade da data limite para o término do prazo final para conclusão do mestrado, e a constatação da importância de trabalhos de revisão, foi decidido que o projeto inicial seria modificado e incrementado com novas variáveis e seria desenvolvido plenamente no curso de doutorado em fluxo contínuo, situação esta prevista no Regimento do Curso de Pós-graduação em Ciências da Saúde.

Esta adequação metodológica está em total concordância com o projeto inicial pelo fato de buscar responder a mesma questão, porém, com um maior número de procedimentos. Destacam-se as mensurações do cortisol plasmático, de dano muscular e de outras variáveis hematológicas e metabólicas, em resposta ao exercício aeróbico agudo, com intensidade imposta e autosselecionada.

A realização deste projeto tem grande importância pelo fato de haver produzido evidência científica, através de uma RS, sobre a influência dos diferentes programas de exercícios físicos na regulação de biomarcadores inflamatórios em mulheres.

É importante ressaltar também a grande evolução intelectual nesta trajetória da realização do curso de mestrado, não apenas devido ao aumento nos conhecimentos acerca do papel dos exercícios físicos como terapia, mas, também, sobre o conhecimento de métodos de desenvolver e de aplicar os princípios da saúde baseada em evidências (SBE), tão conhecidos e valorizados na medicina, na área de Educação Física.

Estes conhecimentos melhoraram muito a atividade docente e de orientação em pesquisa, exercida nos cursos de Bacharelado e de Licenciatura em Educação Física no Centro Universitário do Rio Grande do Norte (UNI-RN). Como exemplo, a produção e execução do Mini-Curso intitulado Revisões Sistemáticas Cochrane, ministrado no XI Congresso de Iniciação Científica do UNI-RN no ano de 2012.

Ainda, as modificações metodológicas descritas anteriormente levaram ao desenvolvimento de um projeto de estudo mais amplo que está em execução e que continuará na expectativa do doutorado em fluxo contínuo que deverá ser iniciado imediatamente após a conclusão do mestrado, neste mesmo programa.

Finalmente, percebe-se por meio deste relato que realizar pesquisa no Brasil é desafiante. Existe capacidade intelectual e vontade de pesquisar. Porém, são necessários maiores investimentos, principalmente em estrutura, por parte dos órgãos governamentais responsáveis, com o objetivo de criar as condições necessárias para que o Brasil conquiste tradição e excelência em sua produção científica.

7. REFERÊNCIAS

1. Murray CJ, Lopez AD. Global mortality, disability, and the contribution of risk factors: Global Burden of Disease Study. Lancet. 1997;349(9063):1436-42.

2. Yach D, Hawkes C, Gould CL, Hofman KJ. The global burden of chronic diseases: overcoming impediments to prevention and control. Jama. 2004;291(21):2616-22.

3. Booth FW, Chakravarthy MV, Spangenburg EE. Exercise and gene expression: physiological regulation of the human genome through physical activity. The Journal of physiology. 2002;543(Pt 2):399-411.

4. Ottaviani E, Valensin S, Franceschi C. The neuro-immunological interface in an evolutionary perspective: the dynamic relationship between effector and recognition systems. Front Biosci. 1998;3:d431-5.

5. Commins SP, Borish L, Steinke JW. Immunologic messenger molecules: cytokines, interferons, and chemokines. The Journal of allergy and clinical immunology. 2010;125(2 Suppl 2):S53-72. Epub 2009/11/26.

6. Black PH. The inflammatory response is an integral part of the stress response: Implications for atherosclerosis, insulin resistance, type II diabetes and metabolic syndrome X. Brain Behav Immun. 2003;17(5):350-64.

7. Black PH. The inflammatory consequences of psychologic stress: relationship to insulin resistance, obesity, atherosclerosis and diabetes mellitus, type II. Med Hypotheses. 2006;67(4):879-91.

8. Fernandez-Real JM, Ferri MJ, Vendrell J, Ricart W. Burden of infection and fat mass in healthy middle-aged men. Obesity (Silver Spring). 2007;15(1):245-52.

9. Calza S, Decarli A, Ferraroni M. Obesity and prevalence of chronic diseases in the 1999-2000 Italian National Health Survey. BMC Public Health. 2008;8:140.

10. Ford ES, Zhao G, Li C, Pearson WS, Mokdad AH. Trends in obesity and abdominal obesity among hypertensive and nonhypertensive adults in the United States. Am J Hypertens. 2008;21(10):1124-8.

11. Patel SB, Reams GP, Spear RM, Freeman RH, Villarreal D. Leptin: linking obesity, the metabolic syndrome, and cardiovascular disease. Curr Hypertens Rep. 2008;10(2):131-7.

12. Ioannidis I. The road from obesity to type 2 diabetes. Angiology. 2008;59(2 Suppl):39S-43S.

13. Pontiroli AE, Frige F, Paganelli M, Folli F. In morbid obesity, metabolic abnormalities and adhesion molecules correlate with visceral fat, not with subcutaneous fat: effect of weight loss through surgery. Obes Surg. 2008.

14. Trevisan M, Bahsas FB, Menotti A. Syndrome X and mortality: a population- based study. Risk factor and life expectancy research group. Am J Epidemiol. 1998;148:958-66.

15. Malik S, Wong ND, Franklin SS, Kamath TV, L'Italien GJ, Pio JR, et al. Impact of the metabolic syndrome on mortality from coronary heart disease, cardiovascular disease, and all causes in United States adults. Circulation. 2004;110:1245-50.

16. Garruti G, Cotecchia S, Giampetruzzi F, Giorgino F, Giorgino R. Neuroendocrine deregulation of food intake, adipose tissue and the gastrointestinal system in obesity and metabolic syndrome. J Gastrointestin Liver Dis. 2008;17(2):193-8.

17. Mujica-Parodi LR, Renelique R, Taylor MK. Higher body fat percentage is associated with increased cortisol reactivity and impaired cognitive resilience in response to acute emotional stress. Int J Obes (Lond). 2009;33(1):157-65. Epub 2008/11/19.

18. Sempere L, Martinez J, de Madaria E, Lozano B, Sanchez-Paya J, Jover R, et al. Obesity and fat distribution imply a greater systemic inflammatory response and a worse prognosis in acute pancreatitis. Pancreatology. 2008;8(3):257-64.

19. Diego VP, Rainwater DL, Wang XL, Cole SA, Curran JE, Johnson MP, et al. Genotype x adiposity interaction linkage analyses reveal a locus on chromosome 1 for lipoprotein-associated phospholipase A2, a marker of inflammation and oxidative stress. Am J Hum Genet. 2007;80(1):168-77.

20. Lavie CJ, Milani RV, Ventura HO. Untangling the heavy cardiovascular burden of obesity. Nat Clin Pract Cardiovasc Med. 2008;5(7):428-9.

21. Heilbronn LK, Campbell LV. Adipose tissue macrophages, low grade inflammation and insulin resistance in human obesity. Curr Pharm Des. 2008;14(12):1225-30.

22. Goossens GH. The role of adipose tissue dysfunction in the pathogenesis of obesity-related insulin resistance. Physiol Behav. 2008;94(2):206-18.

23. Albers R, Antoine JM, Bourdet-Sicard R, Calder PC, Gleeson M, Lesourd B, et al. Markers to measure immunomodulation in human nutrition intervention studies. Br J Nutr. 2005;94(3):452-81.

24. Creely SJ, McTernan PG, Kusminski CM, Fisher M, Da Silva NF, Khanolkar M, et al. Lipopolysaccharide activates an innate immune system response in human adipose tissue in obesity and type 2 diabetes. American journal of physiology Endocrinology and metabolism. 2007;292(3):E740-7.

25. Old LJ. Tumor necrosis factor (TNF). Science. 1985;230(4726):630-2. Epub 1985/11/08.

26. Shirai T, Yamaguchi H, Ito H, Todd CW, Wallace RB. Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor. Nature. 1985;313(6005):803-6. Epub 1985/02/06.

27. Kriegler M, Perez C, DeFay K, Albert I, Lu SD. A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: ramifications for the complex physiology of TNF. Cell. 1988;53(1):45-53. Epub 1988/04/08.

28. Koerner A, Kratzsch J, Kiess W. Adipocytokines: leptin--the classical, resistin-- the controversical, adiponectin--the promising, and more to come. Best practice & research Clinical endocrinology & metabolism. 2005;19(4):525-46. Epub 2005/11/29. 29. Tisdale MJ. Cancer anorexia and cachexia. Nutrition. 2001;17(5):438-42. Epub 2001/05/30.

30. Wray CJ, Mammen JM, Hasselgren PO. Catabolic response to stress and potential benefits of nutrition support. Nutrition. 2002;18(11-12):971-7. Epub 2002/11/15.

31. Chaney SG. Macronutrientes: efeitos metabólicos e implicações para a saúde. In: Devlin TM, editor. Manual de bioquímica com correlações clínicas. São paulo: Blucher; 2011. p. 1252.

32. Field FJ, Watt K, Mathur SN. TNF-alpha decreases ABCA1 expression and attenuates HDL cholesterol efflux in the human intestinal cell line Caco-2. Journal of lipid research. 2010;51(6):1407-15. Epub 2010/01/28.

33. Hotamisligil GS, Peraldi P, Budavari A, Ellis R, White MF, Spiegelman BM. IRS-1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF-alpha- and obesity-induced insulin resistance. Science. 1996;271(5249):665-8. Epub 1996/02/02.

34. Miller JF, Mitchell GF. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. The Journal of experimental medicine. 1968;128(4):801-20. Epub 1968/10/01.

35. Andus T, Geiger T, Hirano T, Northoff H, Ganter U, Bauer J, et al. Recombinant human B cell stimulatory factor 2 (BSF-2/IFN-beta 2) regulates beta- fibrinogen and albumin mRNA levels in Fao-9 cells. FEBS letters. 1987;221(1):18-22. Epub 1987/08/31.

36. Romano M, Sironi M, Toniatti C, Polentarutti N, Fruscella P, Ghezzi P, et al. Role of IL-6 and its soluble receptor in induction of chemokines and leukocyte recruitment. Immunity. 1997;6(3):315-25. Epub 1997/03/01.

37. Febbraio MA, Pedersen BK. Contraction-induced myokine production and release: is skeletal muscle an endocrine organ? Exercise and sport sciences reviews. 2005;33(3):114-9. Epub 2005/07/12.

38. Hiscock N, Chan MH, Bisucci T, Darby IA, Febbraio MA. Skeletal myocytes are a source of interleukin-6 mRNA expression and protein release during contraction: evidence of fiber type specificity. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2004;18(9):992-4. Epub 2004/04/03.

39. Petersen AM, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol. 2005;98(4):1154-62.

40. Mohamed-Ali V, Goodrick S, Rawesh A, Katz DR, Miles JM, Yudkin JS, et al. Subcutaneous adipose tissue releases interleukin-6, but not tumor necrosis factor- alpha, in vivo. The Journal of clinical endocrinology and metabolism. 1997;82(12):4196-200. Epub 1997/12/17.

41. Lyngso D, Simonsen L, Bulow J. Interleukin-6 production in human subcutaneous abdominal adipose tissue: the effect of exercise. The Journal of physiology. 2002;543(Pt 1):373-8. Epub 2002/08/16.

42. Kern PA, Ranganathan S, Li C, Wood L, Ranganathan G. Adipose tissue tumor necrosis factor and interleukin-6 expression in human obesity and insulin resistance. American journal of physiology Endocrinology and metabolism. 2001;280(5):E745-51. Epub 2001/04/05.

43. Tillett WS, Francis T. Serological Reactions in Pneumonia with a Non-Protein Somatic Fraction of Pneumococcus. The Journal of experimental medicine. 1930;52(4):561-71. Epub 1930/09/30.

44. Macleod CM, Avery OT. The Occurrence during Acute Infections of a Protein Not Normally Present in the Blood : Iii. Immunological Properties of the C-Reactive

Protein and Its Differentiation from Normal Blood Proteins. The Journal of experimental medicine. 1941;73(2):191-200. Epub 1941/01/31.

45. Macleod CM, Avery OT. The Occurrence during Acute Infections of a Protein Not Normally Present in the Blood : Ii. Isolation and Properties of the Reactive Protein. The Journal of experimental medicine. 1941;73(2):183-90. Epub 1941/01/31. 46. Abernethy TJ, Avery OT. The Occurrence during Acute Infections of a Protein Not Normally Present in the Blood : I. Distribution of the Reactive Protein in Patients' Sera and the Effect of Calcium on the Flocculation Reaction with C Polysaccharide of Pneumococcus. The Journal of experimental medicine. 1941;73(2):173-82. Epub 1941/01/31.

47. Pepys MB, Dash AC, Fletcher TC, Richardson N, Munn EA, Feinstein A. Analogues in other mammals and in fish of human plasma proteins, C-reactive protein and amyloid P component. Nature. 1978;273(5658):168-70. Epub 1978/05/11.

48. Volanakis JE, Kaplan MH. Specificity of C-reactive protein for choline phosphate residues of pneumococcal C-polysaccharide. Proceedings of the Society for Experimental Biology and Medicine Society for Experimental Biology and Medicine. 1971;136(2):612-4. Epub 1971/02/01.

49. Kushner I, Rzewnicki D, Samols D. What does minor elevation of C-reactive protein signify? The American journal of medicine. 2006;119(2):166 e17-28. Epub 2006/01/31.

50. Hutchinson WL, Noble GE, Hawkins PN, Pepys MB. The pentraxins, C- reactive protein and serum amyloid P component, are cleared and catabolized by hepatocytes in vivo. The Journal of clinical investigation. 1994;94(4):1390-6. Epub 1994/10/01.

51. Vigushin DM, Pepys MB, Hawkins PN. Metabolic and scintigraphic studies of radioiodinated human C-reactive protein in health and disease. The Journal of clinical investigation. 1993;91(4):1351-7. Epub 1993/04/01.

52. Calabro P, Chang DW, Willerson JT, Yeh ET. Release of C-reactive protein in response to inflammatory cytokines by human adipocytes: linking obesity to vascular inflammation. Journal of the American College of Cardiology. 2005;46(6):1112-3. Epub 2005/09/20.

53. Tsatsoulis A, Fountoulakis S. The protective role of exercise on stress system dysregulation and comorbidities. Ann N Y Acad Sci. 2006;1083:196-213.

54. Elenkov IJ, Chrousos GP. Stress hormones, Th1/Th2 patterns, pro/anti- inflammatory cytokines and susceptibility to disease. Trends Endocrinol Metab. 1999;10(9):359-68.

55. Myers J, Kaykha A, George S, Abella J, Zaheer N, Lear S, et al. Fitness versus physical activity patterns in predicting mortality in men. The American journal of medicine. 2004;117(12):912-8.

56. Hilberg T. Physical activity in the prevention of cardiovascular diseases. Epidemiology and mechanisms. Hamostaseologie. 2008;28(1-2):9-12, 4-5. Korperliche Aktivitat in der Pravention kardiovaskularer Erkrankungen. Epidemiologie und Mechanismen.

57. Silva TS, Longui CA, Faria CD, Rocha MN, Melo MR, Faria TG, et al. Impact of prolonged physical training on the pituitary glucocorticoid sensitivity determined by very low dose intravenous dexamethasone suppression test. Horm Metab Res. 2008;40(10):718-21.

58. Skilton MR, Sieveking DP, Harmer JA, Franklin J, Loughnan G, Nakhla S, et al. The effects of obesity and non-pharmacological weight loss on vascular and ventricular function and structure. Diabetes Obes Metab. 2008;10(10):874-84.

59. Smith DT, Carr LJ, Dorozynski C, Gomashe C. Internet-delivered lifestyle physical activity intervention: limited inflammation and antioxidant capacity efficacy in overweight adults. J Appl Physiol. 2008;106(1):49-56.

60. Nieman DC. Marathon training and immune function. Sports Med. 2007;37(4- 5):412-5.

61. Whitham M, Fortes MB. Heat shock protein 72: release and biological significance during exercise. Front Biosci. 2008;13:1328-39.

62. Stranahan AM, Lee K, Mattson MP. Central mechanisms of HPA axis regulation by voluntary exercise. Neuromolecular Med. 2008;10(2):118-27.

63. Robson-Ansley PJ, Blannin A, Gleeson M. Elevated plasma interleukin-6 levels in trained male triathletes following an acute period of intense interval training. Eur J Appl Physiol. 2007;99(4):353-60.

64. Tuan TC, Hsu TG, Fong MC, Hsu CF, Tsai KK, Lee CY, et al. Deleterious effects of short-term, high-intensity exercise on immune function: evidence from leucocyte mitochondrial alterations and apoptosis. Br J Sports Med. 2008;42(1):11-5. 65. Kampus P, Kals J, Unt E, Zilmer K, Eha J, Teesalu R, et al. Association between arterial elasticity, C-reactive protein and maximal oxygen consumption in

well-trained cadets during three days extreme physical load: a pilot study. Physiol Meas. 2008;29(4):429-37.

66. Campbell JE, Rakhshani N, Fediuc S, Bruni S, Riddell MC. Voluntary wheel running initially increases adrenal sensitivity to adrenocorticotrophic hormone, which is attenuated with long-term training. J Appl Physiol. 2009;106(1):66-72. Epub 2008/11/15.

67. Sasse SK, Greenwood BN, Masini CV, Nyhuis TJ, Fleshner M, Day HE, et al. Chronic voluntary wheel running facilitates corticosterone response habituation to repeated audiogenic stress exposure in male rats. Stress. 2008;11(6):425-37. Epub 2008/12/10.

68. Pedersen BK, Toft AD. Effects of exercise on lymphocytes and cytokines. Br J Sports Med. 2000;34(4):246-51.

69. Roberts CK, Won D, Pruthi S, Kurtovic S, Sindhu RK, Vaziri ND, et al. Effect of a short-term diet and exercise intervention on oxidative stress, inflammation, MMP-9, and monocyte chemotactic activity in men with metabolic syndrome factors. J Appl Physiol. 2006;100:1657-65.

70. Nojima H, Watanabe H, Yamane K, Kitahara Y, Sekikawa K, Yamamoto H, et al. Effect of aerobic exercise training on oxidative stress in patients with type 2 diabetes mellitus. Metabolism. 2008;57(2):170-6.

71. Kim MK, Tomita T, Kim MJ, Sasai H, Maeda S, Tanaka K. Aerobic exercise training reduces epicardial fat in obese men. J Appl Physiol. 2008.

72. Edwards KM, Ziegler MG, Mills PJ. The potential anti-inflammatory benefits of improving physical fitness in hypertension. J Hypertens. 2007;25(8):1533-42.

73. Handschin C, Spiegelman BM. The role of exercise and PGC1alpha in inflammation and chronic disease. Nature. 2008;454(7203):463-9. Epub 2008/07/25. 74. Kullo IJ, Khaleghi M, Hensrud DD. Markers of inflammation are inversely associated with VO2 max in asymptomatic men. J Appl Physiol. 2007;102(4):1374-9. 75. Vieira VJ, Valentine RJ, McAuley E, Evans E, Woods JA. Independent relationship between heart rate recovery and C-reactive protein in older adults. J Am Geriatr Soc. 2007;55(5):747-51.

76. Febbraio MA, Pedersen BK. Muscle-derived interleukin-6: mechanisms for activation and possible biological roles. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2002;16(11):1335-47.

77. Aronson D, Avizohar O, Levy Y, Bartha P, Jacob G, Markiewicz W. Factor analysis of risk variables associated with low-grade inflammation. Atherosclerosis. 2008;200(1):206-12.

78. Artham SM, Lavie CJ, Milani RV, Ventura HO. The obesity paradox: impact of obesity on the prevalence and prognosis of cardiovascular diseases. Postgrad Med. 2008;120(2):34-41.

79. Bradley RL, Jeon JY, Liu FF, Maratos-Flier E. Voluntary exercise improves insulin sensitivity and adipose tissue inflammation in diet-induced obese mice. American journal of physiology Endocrinology and metabolism. 2008;295(3):E586- 94. Epub 2008/06/26.

80. Keylock KT, Vieira VJ, Wallig MA, DiPietro LA, Schrementi M, Woods JA. Exercise accelerates cutaneous wound healing and decreases wound inflammation in aged mice. Am J Physiol Regul Integr Comp Physiol. 2008;294(1):R179-84.

81. Hoffman-Goetz L, Spagnuolo PA, Guan J. Repeated exercise in mice alters expression of IL-10 and TNF-alpha in intestinal lymphocytes. Brain Behav Immun. 2008;22(2):195-9.

82. Glund S, Krook A. Role of interleukin-6 signalling in glucose and lipid metabolism. Acta Physiol (Oxf). 2008;192(1):37-48.

83. Booth F, Roberts CK. Linking performance and chronic disease risk: indices of physical performance are surrogates for health. Br J Sports Med. 2008;42:950-2. 84. Helge JW, Damsgaard R, Overgaard K, Andersen JL, Donsmark M, Dyrskog SE, et al. Low-intensity training dissociates metabolic from aerobic fitness. Scand J Med Sci Sports. 2008;18(1):86-94.

85. Cooper DM, Radom-Aizik S, Schwindt CD, Zaldivar F. Dangerous Exercise -- Lessons Learned from Dysregulated Inflammatory Responses to Physical Activity. J Appl Physiol. 2007.

86. Tjonna AE, Lee SJ, Rognmo O, Stolen TO, Bye A, Haram PM, et al. Aerobic interval training versus continuous moderate exercise as a treatment for the metabolic syndrome: a pilot study. Circulation. 2008;118(4):346-54.

87. Haram PM, Kemi OJ, Lee SJ, Bendheim MO, Al-Share QY, Waldum HL, et al. Aerobic interval training vs. continuous moderate exercise in the metabolic syndrome of rats artificially selected for low aerobic capacity. Cardiovasc Res. 2008.

88. Butterfield TA, Best TM, Merrick MA. The dual roles of neutrophils and macrophages in inflammation: a critical balance between tissue damage and repair. J Athl Train. 2006;41(4):457-65.

89. Schjerve IE, Tyldum GA, Tjonna AE, Stolen T, Loennechen JP, Hansen HE, et al. Both aerobic endurance and strength training programmes improve cardiovascular health in obese adults. Clin Sci (Lond). 2008;115(9):283-93.

90. Tambalis KD, Panagiotakos DB, Kavouras SA, Sidossis LS. Responses of blood lipids to aerobic, resistance, and combined aerobic with resistance exercise training: a systematic review of current evidence. Angiology. 2008.

91. Daussin FN, Zoll J, Dufour SP, Ponsot E, Lonsdorfer-Wolf E, Doutreleau S, et al. Effect of interval versus continuous training on cardiorespiratory and mitochondrial functions: relationship to aerobic performance improvements in sedentary subjects. Am J Physiol Regul Integr Comp Physiol. 2008;295(1):R264-72.

92. Lacasa D, Taleb S, Keophiphath M, Miranville A, Clement K. Macrophage- secreted factors impair human adipogenesis: involvement of proinflammatory state in preadipocytes. Endocrinology. 2007;148(2):868-77.

93. Lazar MA. How obesity causes diabetes: not a tall tale. Science. 2005;307(5708):373-5.

94. Bengmark S. Impact of nutrition on ageing and disease. Curr Opin Clin Nutr Metab Care. 2006;9(1):2-7.

95. Meta-Análise e Revisão Sistemática [database on the Internet]. Atheneu. 2010.

96. Clarke M, Horton R. Bringing it all together: Lancet-Cochrane collaborate on systematic reviews. Lancet. 2001;357(9270):1728.

97. Guyatt GH, Sackett DL, Sinclair JC, Hayward R, Cook DJ, Cook RJ. Users' guides to the medical literature. IX. A method for grading health care recommendations. Evidence-Based Medicine Working Group. Jama. 1995;274(22):1800-4.

98. Higgins JPT, Green S. Cochrane Handbook for Systematic Reviews of Interventions. Version 5.1.0 [updated March 2011] ed: The Cochrane Collaboration; 2011. Disponível em: www.cochrane-handbook.org.

99. Verhagen AP, de Vet HC, de Bie RA, Kessels AG, Boers M, Bouter LM, et al. The Delphi list: a criteria list for quality assessment of randomized clinical trials for conducting systematic reviews developed by Delphi consensus. J Clin Epidemiol

[Internet]. 1998 J Clin Epidemiol; 51:[1235-41 pp.]. Available from: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Cita tion&list_uids=10086815

100. Schulz KF, Altman DG, Moher D, Group C. CONSORT 2010 statement: updated guidelines for reporting parallel group randomised trials. Bmj. 2010;340:c332. Epub 2010/03/25.