The IAEA response and the expert mission

Esta pesquisa contribuiu tanto com a área cosmética como ambiental, apresentando uma nova matéria-prima para indústria cosmética, a partir de um produto de descarte do subproduto do sisal, o que pode contribuir em um futuro próximo com o desenvolvimento ecológico, diminuição do impacto ambiental e socioeconômico das regiões que cultivam a Agave sisalana. Esta pesquisa também possibilitou demonstrar a aplicabilidade dessa matéria-prima em um produto cosmético, produto este, que seguiu a tendência da área de cosmetologia, no que tange aos aspectos nanotecnológicos. Por fim, e para consagrar o caráter inovador e inédito do projeto desenvolvido, foi demonstrada a eficácia in vitro antioxidante e in vivo hidratante, contemplando assim, grande parte da cadeia produtiva de um produto cosmético e também agregando valor à cadeia produtiva de Agave sisalana.

Em adição, este trabalho também contribuiu para formação de recursos humanos especializados na área cosmética, incluindo alunos de iniciação científica com trabalho apresentado e premiado como primeiro lugar na categoria Engenharias e Meio Ambiente no Congresso de Iniciação Científica da UFRN. Finalmente, essa pesquisa resultou em produção científica aprovada em congressos internacionais, inclusive com apresentação oral, e em um conteúdo científico que culminará com a publicação de dois artigos científicos em periódicos indexados internacionalmente.

Ainda, a abordagem ambiental trazida neste trabalho cria novas perspectivas para utilização de resíduos de descarte industriais como matérias-primas úteis e comerciáveis, inclusive para futuros projetos utilizando o resíduo de Agave sisalana com foco na eficácia fotoprotetora e antienvelhecimento.

REFERÊNCIAS

ABBAS, A. et al. Process optimization of ultrasound-assisted curcumin

nanoemulsions stabilized by OSA-modified starch. Ultrasonics Sonochemistry, v. 21 p.1265–1274, 2014.

ADDOR, F. Avaliação de Segurança de Cosméticos: estado da arte. Cosmetics & Toiletries, v. 28, n. 6, p. 50-52, 2016.

ADJONU, R. et al. Whey protein peptides as components of nanoemulsions: A review of emulsifying and biological functionalities. Journal of Food Engineering, v. 122, p. 15–27, 2014.

AGÊNCIA SENADO. Projeto que restringe uso de animais em testes de cosméticos ainda longe de consenso. Disponível em:

<http://www12.senado.leg.br/noticias/materias/2015/05/26/projeto-que-restringe-uso- de-animais-em-testes-de-cosmeticos-ainda-longe-de-consenso>. Acesso em: 04 jan. 2017

AHUMADA-SANTOS Y. P. et al. Chemical characterization, antioxidant and antibacterial activities of six Agave species from Sinaloa, Mexico. Industrial Crops and Products, v. 49, p. 143-149, 2013.

ALIMI, H. et al. Evaluation of antioxidant and antiulcerogenic activities of Opuntia ficus indica f. inermis flowers extract in rats. Environmental Toxicology and Pharmacology, v. 3 2, p. 406–416, 2011.

ALONSO , C.et al. An ex vivo methodology to assess the lipid peroxidation in stratum corneum. Journal of Photochemistry and Photobiology B: Biology, v.97, p. 71–76, 2009.

ALVES, V. M.et al. Predicting chemically-induced skin reactions. Part I: QSAR models of skin sensitization and their application to identify potentially hazardous compounds. Toxicology and Applied Pharmacology, v. 284, p. 262–272, 2015a.

ALVES, V. M. et al. Predicting chemically-induced skin reactions. Part II: QSAR models of skin permeability and the relationships between skin permeability and skin sensitization. Toxicology and Applied Pharmacology, v. 284, p. 273–280, 2015b.

AMARAL-MACHADO, L. et al. New Trends on Antineoplastic Therapy Research: Bullfrog (Rana catesbeiana Shaw) Oil Nanostructured Systems. Molecules, v. 21, n. 585, p. 1-16, 2016.

ANTIGNAC, L. et al. Safety of botanical ingredients in personal care

products/cosmetics. Food and Chemical Toxicology, v. 49, p. 324–341, 2011.

ARDA, O.; GÖKSÜGÜR, N.; TÜZÜN, Y. Basic histological structure and functions of facial skin. Clinics in Dermatology, v. 32, p. 3-13, 2014.

ARRIZON, J. et al. Comparison of the water-soluble carbohydrate composition and fructan structures of Agave tequilana plants of different ages. Food Chemistry, v.122, p. 123–130, 2010.

ATHUKORALA, Y.; KIM, K. N.; JEON, Y. J. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food and Chemical Toxicology, v. 44, n. 7, p. 1065-1074, 2006.

BALI, V.; ALI, M.; ALI, J. Study of surfactant combinations and development of a novel nanoemulsion for minimising variations in bioavailability of ezetimibe. Colloids and Surfaces B: Biointerfaces, v. 76, p. 410–420, 2010.

BARCO, D.; GIMÉNEZ-ARNAU, Y. A. Xerosis: una disfunción de la barrera epidérmica. Actas Dermosifiliogr, v. 2008, n. 99, p. 671-82, 2008.

BARONI, A. et al. Structure and function of the epidermis related to barrier properties. Clinics in Dermatology, v. 30, p. 257–262, 2012.

BONTÉ, F. Skin moisturization mechanisms: New data. Annales Pharmaceutiques Françaises, v. 69, p. 135-141, 2011.

BRAZILIAN CENTER FOR VALIDATION OF ALTERNATIVE METHODS. A Proposal to Establish a Brazilian Center for Validation of Alternative Methods (BraCVAM) Disponível em:

<https://www.incqs.fiocruz.br/index.php?option=com_content&view=article&id=1188 &Itemid=214>. Acesso em: 24 ago.2016.

BRASIL, Agência Nacional de Vigilância Sanitária. Guia de estabilidade de produtos cosméticos. Brasília: Anvisa, 2005.

BRASIL, Agência Nacional de Vigilância Sanitária. Guia para avaliação de Segurança de Produtos Cosméticos.2 ed. Brasília: Anvisa, 2012.

BRASIL. Ministério da Ciência, Tecnologia e Inovação. Portaria. N° 491, de 3 de julho de 2012. Institui a Rede Nacional de Métodos Alternativos - RENAMA e sua estrutura no âmbito do Ministério da Ciência, Tecnologia e Inovação - MCTI, que será supervisionada por um Conselho Diretor. Diario Oficial da União, Brasília, n. 129, p. 19, 2012.

BRASIL. Lei n. 11.794, de 08 de outubro de 2008. Regulamenta o inciso VII de parágrafo 1º do art. 225 da Constituição Federal, estabelecendo procedimentos para o uso científico de animais; revoga a Lei n. 6.638, de 8 de maio de 1979; e dá

outras providências. Diário Oficial da República Federativa do Brasil, Brasília, 09 de outubro de 2008.

BRASIL. Decreto n. 6.899, de 15 de julho de 2009a. Dispõe sobre a composição do Conselho Nacional de Controle de Experimentação Animal - CONCEA, estabelece as normas para o seu funcionamento e de sua Secretaria-Executiva, cria o Cadastro das Instituições de Uso Científico de Animais - CIUCA, mediante a regulamentação da Lei no 11.794, de 8 de outubro de 2008, que dispõe sobre procedimentos para o uso científico de animais, e dá outras providências.Diário Oficial da República Federativa do Brasil, Brasília, 16 de julho de 2009.

BRADFORD, M.M. A Rapid and Sensitive Method for the Quantitation Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, v.72, n. 1-2, p. 248-254, 1976.

BRANCO, A. et al. d-Mannitol from Agave sisalana biomass waste. Industrial Crops and Products, v. 32, p. 507–510, 2010.

BRAND-WILLIAMS, W.; CUVELIER, M. E.; BERSET, C. Use of a Free Radical Method to Evaluate Antioxidant Activity. Lebensmittel-Wissenschaft und- Technologie, v. 28, n. 1, p. 25-30, 1995.

BOTURA, M. B. et al. In vitro ovicidal and larvicidal activity of Agave sisalana Perr. (sisal) on gastrointestinal nematodes of goats. Veterinary Parasitology, v. 192, p. 211-217, 2013.

BYRNE, A. J. Bioengineering and subjective approaches to the clinical evaluation of dry skin. International Journal of Cosmetic Science, v. 32, p. 410–421, 2010.

CAMARGO JUNIOR, F. B.; GASPAR, L. R.; MAIA CAMPOS, P. M. B. G. Immediate and long-term effects of polysaccharides-based formulations on human skin.

Brazilian Journal of Pharmaceutical Sciences, v. 8, n. 3, p. 548-555, 2012.

CARMONA-JIMENEZ, Y. et al. Simplification of the DPPH assay for estimating the antioxidant activity of wine and wine by-products. Food Chemistry, v. 165, p. 198– 204, 2014.

CARPENTER,J; SAHARAN, V. K. Ultrasonic assisted formation and stability of mustard oil in water nanoemulsion: Effect of process parameters and their optimization. Ultrasonics Sonochemistry, v. 35, parte A, p. 422-430, 2017. CAROCHO, M; FERREIRA, I. C.F.R. A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food and Chemical Toxicology, v. 51, p. 15- 25, 2013.

CHEN, P. Y. et al. Isolation and Immunomodulatory Effect of Homoisoflavones and Flavones from Agave sisalana Perrine ex Engelm. Molecules, v. 1, p.1789-1795, 2009.

CHORILLI, M. Desenvolvimento e caracterização físico-química de sistemas nanoestruturados contendo palmitato de retinol: controle microbiológico, avaliação da segurança e eficácia no tratamento do envelhecimento cutâneo. 2007. 174 f. Tese (Doutorado em Ciências Farmacêuticas) - Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Farmacêuticas. Araraquara, 2007. CIR – Cosmetic Ingredient Review. Disponível em: < http://www.cir-

safety.org/ingredients. > Acesso em: nov. 2014.

CONAB. Companhia Nacional de Abastecimento. Sisal: exportações em queda.Disponível em:

http://www.conab.gov.br/OlalaCMS/uploads/arquivos/16_07_28_17_46_03_sisal_co njuntura_jul_2016.pdf. Acesso em: 30.01.2017.

COSING –. European Commission Health and Consumers – Cosmetics Ingredients & Substances. Disponível

em:<http://ec.europa.eu/consumers/cosmetics/cosing/index.cfm?fuseation=search.si mple> Acesso em: 05 nov. 2014.

COURAGE KHAZAKA. Cologne, Alemanha. 2014. Disponível em: <

http://www.courage-khazaka.de/index.php/en/all-downloads/downloads-en > . Acesso em: Jul. 2014.

DAMASCENO, G.A.B. et al. Use of Opuntia ficus-indica (L.) Mill extracts from Brazilian Caatinga as an alternative of natural moisturizer in cosmetic formulations. Brazilian Journal of Pharmaceutical Sciences, vol. 52, n. 3, 2016.

DAITHANKAR, A. V. Moisturizing efficiency of silk protein hydrolysate: Silk fibroin. Indian Journal of Biotechnology, v. 4, p. 115-121, 2005.

DAL'BELO, S. E.; GASPAR, L. R.; MAIA CAMPOS, P. M. B. G. Moisturizing effect of cosmetic formulations containing Aloe vera extract in different concentrations

assessed by skin bioengineering techniques. Skin Research and Technology, v. 12, n. 4, p. 241-246, 2006.

DARLENSKI, R. et al. Non-invasive in vivo methods for investigation of the skin barrier physical properties. European Journal of Pharmaceutics and

Biopharmaceutics, v. 72, n. 2, p. 295-303, 2009.

DAVIS, H. M. Analysis of creams and lotions. In: SENZEL, A. J. (Ed.). Newburger’s manual of cosmetic analysis. Washington: Association of Official Analytical Chemists, 1977. cap.4, p.32.

DONG, H. et al. Antioxidant activity and chemical compositions of essential oil andethanol extract of Chuanminshen violaceum. Industrial Crops and Products, v. 76, p. 290–297, 2015.

DRAELOS, Z. D. Therapeutic Moisturizers. Dermatologic Clinics, v. 18, n. 4, p. 597- 607, 2000.

DRENO, B. et al. The science of dermocosmetics and its role in dermatology. Journal of the European Academy of Dermatology and Venereology, v. 28, n. 11, p. 1409- 1417, 2014.

DUBOIS, M. et al. Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, v. 28, n. 3, p. 350-356, 1956.

DUNDER, RJ et al. Anti-inflammatory and analgesic potential of hydrolyzed extract of Agave sisalana Perrine ex Engelm. Asparagaceae. Brazilian Journal of

Pharmacognosy, v. 20, n. 2, p. 376-381, 2010.

FELIPPI, C. C. Safety and Efficacy of Antioxidants-Loaded Nanoparticles for an Anti- Aging Application. Journal of Biomedical Nanotechnology, v, 8, p. 316–321, 2012.

FREITAS, J. V.; GASPAR, L. R. In vitro photosafety and efficacy screening of

apigenin, chrysin and beta-carotene for UVA and VIS protection. European Journal of Pharmaceutical Sciences, v. 89, p. 146–153, 2016.

FRESHNEY, R. I. Culture of animal cells: a manual of basic technique and specialized applications. 6 ed. Nova Jersey: Wiley-Blackwell, 2010, 732p. RIBEIRO, C. Cosmetologia aplicada a dermatoestética. 2 ed. São Paulo: Pharmabooks, 2010. 441p.

FIDELIS, G. P. et al. Proteolysis, NaOH and Ultrasound-Enhanced Extraction of Anticoagulant and Antioxidant Sulfated Polysaccharides from the Edible Seaweed, Gracilaria birdiae. Molecules, v. 19, p. 18511-18526, 2014.

FLUHR, J. W.; FEINGOLD, K. R.; ELIAS, P. M. Transepidermal water loss reflects permeability barrier status: validation in human and rodent in vivo and ex vivo models. Experimental Dermatology, v. 15, n. 7, p. 483-492, 2006.

FLUHR, J. W. Pratical aspects of cosmetic testing. Berlin Heidelberg: Springler Press, 2011.

FUTRAKUL B, KANLAYAVATTANAKUL M, KRISDAPHONG P. Biophysic evaluation of polysaccharide gel from durian’s fruit hulls for skin moisturizer. International

Journal of Cosmetic Science, v. 32, n. 3, p. 211-215, 2010.

GARBOSSA, W. A.; MAIA CAMPOS, P. M. B. G. Euterpe oleracea, Matricaria chamomilla, and Camellia sinensis aspromising ingredients for development of skin care formulations. Industrial Crops and Products, v.83, p. 1–10, 2016.

GANESAN, P.; CHOI, D-K. Current application of phytocompound-based nanocosmeceuticals for beauty and skin therapy. International Journal of Nanomedicine, v. 11, p. 1987-2007, 2016.

GIANETE, M. D. et al. The Use of Nanotechnology in Cosmetic Formulations: The Influence of Vehicle in the Vitamin A Skin Penetration.Current Nanoscience, v. 8, p. 526-534, 2012.

GIANETE, M. D; MAIA CAMPOS, P. M. B. G. Avaliação da eficácia de formulações cosméticas contendo extratos proteicos de trigo e soja por técnicas de biofísica e microscopia confocal a laser. Revista Brasileira de Medicina, Especial Dermatologia 1, p. 12-18, 2012.

GRIFFIN, W. C. Classification of surface-active agents by "hlb".Journal Society Cosmetic Chemistry. 1, p. 311-326, 1949.

GUARIENTI, C.; BERTOLIN, T. E.; COSTA, J. A. V. Capacidade antioxidante da microalga Spirulina platensis em células da levedura Saccharomyces cerevisiae submetidas ao estressor paraquat. Revista do Instituto Adolfo Lutz, v. 69, n. 1, p. 106-111, 2010.

GUPTA, A. et al. Nanoemulsions: formation, properties and applications, Soft Matter, v. 12, p. 2826—2841, 2016.

GUTIERREZ, A.; RODRIGUEZ, I. M.; RIO, J. C. Chemical composition of lipophilic extractives from sisal (Agave sisalana) fibers. Industrial Crops and Products, v. 28, p. 81-87, 2008.

HARRIS, M. I. N. D. C. Pele: estrutura, propriedades e envelhecimento. São Paulo: Senac, 2003. 165 p.

INTERAGENCY COORDINATING COMMITTEE ON THE VALIDATION OF ALTERNATIVE METHODS.. Peer Review Panel Report: the use of in vitro basal cytotoxicity test methods for estimating starting doses for acute oral systemic toxicity testing. Bethesda: National Institutes of Health, 2006. 62p. Disponível em:

<http://iccvam.niehs.nih.gov/docs/acutetox_docs/ATpanelrpt06/ATpanelrpt.pdf>. Acesso em: 04 abr. 2013.

INTERNATIONAL ORGANIZATION FOR STANDARDIZATION. ISO 10993-5: biological evaluation of medical devices - Part 5: Tests for cytotoxicity: in vitro methods. Geneva: ISO, 2009. 34p.

INVASIVE SPECIES COMPENDIUM. Agave sisalana (sisal hemp). Disponível em: <www.cabi.org/isc/datasheet/3855; cirtec123>.Acesso: 06 jul. 2016.

IZQUIERDO, P. et al. Formation and stability of nano-emulsions prepared using the phase inversion temperature method. Langmuir, v.18, n.01, p. 26-30, 2002.

JAFARI, S. M.; HE, Y. H.; BHANDARI, B. Nano-emulsioin production by sonication and microfluidization – a comparison. International Journal of Food Properties, v. 9, n, 3, p. 475-85, 2006.

KABRI, T-H. et al. Physico-chemical characterization of nano- emulsions in cosmetic matrix enriched on omega-3. Journal of Nanobiotechnology, n. 9, v. 41, 2011.

KOCAK, M. S. et al. Salvia cadmica: Phenolic composition and biological activity. Industrial Crops and Products, v. 85, p. 204–212, 2016.

KOMAIKO, J.; McCLEMENTS, D. J. Optimization of isothermal low-energy

nanoemulsion formation: Hydrocarbon oil, non-ionic surfactant, and water systems. Journal of Colloid and Interface Science, v. 425, p. 59-66, 2014.

KHLEBNIKOV, A. I. et al. Improved quantitative structure–activity relationship models to predict antioxidant activity of flavonoids in chemical, enzymatic, and cellular

systems. Bioorganic & Medicinal Chemistry, v. 15, p. 1749–1770, 2007.

KLANG, V. et al. Electron microscopy of nanoemulsions: anessential tool for characterization and stability assessment. Micron, v. 43, p. 85–103, 2012.

KLIGMAN, A. M.; WOODING, W. M. A method for the measurement and evaluationof irritants of human skin. Journal of Investigative Dermatology, v.49, p.78-94, 1967. KUMAR, K. L. S. et al. Integrated Computational Solution for Predicting Skin Sensitization Potential of Molecules. Plos one, v. 11, n. 6, p. 1-22, 2016.

LASTDRAGER, J.; HANSON, J.; SMEEKENS, S. Sugar signals and the control of plant growth and development. Journal of Experimental Botany, v. 65, n. 3, p. 799- 807, 2014.

LEONG, T. S. H. et al. Minimising oil droplet size using ultrasonic emulsification. Ultrasonics Sonochemistry, v. 16, p. 721–727, 2009.

LEOPOLDINI, M.; RUSSO, N.; TOSCANO, M. The molecular basis of working

mechanism of natural polyphenolic antioxidants. Food Chemistry, v.125, p. 288–306, 2011.

LIN, K. W. et al. Antioxidant prenylated phenols of Artocarpus plants attenuate ultraviolet radiation-induced damage on human keratinocytes and fibroblastos. Phytochemistry Letters, v. 14, p. 190–197, 2015.

LIVINGSTON, D. P.; HINCHA, D. K.; HEYER, A. G. Fructan and its relationship to abiotic stress tolerance in plants. Cellular and Molecular Llife Sciences, v.66, n. 13, p. 2007-2023, 2009.

LO, I. et al. The influence of surfactant HLB and the nature of the oil phase on the phase diagrams of nonionic surfactant – oil – water system. Journal of Colloid and Interface Science, v.59, n.2, p. 319-327, 1977.

LODÉN, M. Interactions between the stratum corneum and topically applied

products: regulatory, instrumental and formulation issues with focus on moisturizers. British Journal of Dermatology, v. 17, Suppl. 3, p. 38–44, 2014.

LODÉN, M. Effect of moisturizers on epidermal barrier function. Clinics in Dermatology, v. 30, p. 286–296, 2012.

LU, W-C. et al.Skin permeation of D-limonene-based nanoemulsions as a transdermal carrier prepared by ultrasonic emulsification. Ultrasonics Sono Chemistry, v. 21, p. 826–832, 2014.

LUO, X. et al. Fabrication of β-carotene nanoemulsion-based delivery systems using dual-channel microfluidization: Physical and chemical stability. Journal of Colloid and Interface Science, v. 490, p. 328–335, 2017.

MAIA CAMPOS, P. M. B. G. et al. Application of tetra-isopalmitoyl ascorbic acid in cosmetic formulations: Stability studies and in vivo efficacy. European Journal of Pharmaceutics and Biopharmaceutics, v. 82, n.3, p. 580–586, 2012.

MARAN, J. P.; PRIYA, B. Ultrasound-assisted extraction of pectin from sisal waste. Carbohydrate Polymers, v. 115, p. 732–738, 2015.

MARTIN, A. R. et al. Caracterização química e estrutural da fibra de sisal da variedade Agave sisalana. Polímeros, v. 19, n. 1, p. 40-46, 2009.

McCLEMENTS, D.J. Edible nanoemulsions: fabrication, properties, and functional performance. Soft Matter, v. 7, p. 2297–2316, 2011.

McCLEMENTS, D.J.; RAO, J. Food-Grade Nanoemulsions:Formulation, Fabrication, Properties, Performance, Biological Fate, and Potential Toxicity. Food Science and Nutrition, v. 51, p. 285-330, 2011.

McCLEMENTS, D.J. Nanoemulsions versus microemulsions: Terminology, differences and similarities. Soft Matter, v. 8, p.1719-1729, 2012.

MAHDI, E. S. et al. Formulation and in vitro release evaluation of newly synthesized palm kernel oil esters-based nanoemulsion delivery system for 30% ethanolic dried extract derived from local Phyllanthus urinaria for skin antiaging. International Journal of Nanomedicine, v. 6, p. 2499–2512, 2011.

MELO, M. O.; MAIA CAMPOS, P. M. B. G. Função de barreira da pele e pH cutâneo. Cosmetics & Toiletries, v. 28, n. 3, 2016a.

MELO, M. O.; MAIA CAMPOS, P. M. B. G.Técnicas para avaliar a hidratação e a oleosidade da pele. Cosmetics & Toiletries , v. 28, n. 2, 2016b.

MELO-SILVEIRA, R. F. et al. In Vitro Antioxidant, Anticoagulant and Antimicrobial Activity and in Inhibition of Cancer Cell Proliferation by Xylan Extracted from Corn Cobs. International Journal of Molecular Sciences, v. 13, p. 409-426, 2012.

MORS, W. B.; SHAPARIN, N. A obtenção de esteroides do sisal. Revista Brasileira de Tecnologia, v. 4, p. 153-167, 1974.

NEGREIROS, M. M. F. Nanopartículas de prata contendo polissacarídeos sulfatados de algas: caracterização química, morfológica e identificação de suas atividades antioxidantes, bactericidas, antiproliferativa e imunomodulatória. 2015.

143f.Dissertação (Mestrado em Bioquímica) – Universidade Federal do Rio Grande do Norte, Natal, 2015.

NGO, M. A.; MAIBACH, H. I. Dermatotoxicology: Historical perspective and advances. Toxicology and Applied Pharmacology, v. 243, p. 225–238, 2010. NOHYNEK, G. J.; ANTIGNAC, E.; RE, T. Herve Toutain Safety assessment of personal care products/cosmetics and their ingredients. Toxicology and Applied Pharmacology, v. 243, p. 239–259, 2010.

ODRIOZOLA-SERRANO, I. et al. Antioxidant activity of thermal or non thermally treated strawberry and mango juices by Saccharomyces cerevisiae growth based assays. LWT - Food Science and Technology, v. 74, p. 55-61, 2016.

OLIVEIRA, G. K. F. et al. Batch-injection analysis with amperometric detection of the DPPH radical for evaluation of antioxidante capacity. Food Chemistry, v. 192, p. 691–697, 2016.

ORGANIZATION FOR ECONOMIC CO-OPERATION AND DEVELOPMENT

(OECD). Guideline for testing of chemicals: n. 432 – In vitro 3T3 NRU. Phototoxicity Test. 2004. Disponível em: < http://www.oecd-ilibrary.org/environment/oecd-

guidelines-for-the-testing-of-chemicals-section-4-health-effects_20745788> Acesso em: 19 abr. 2016.

ORGANIZATION FOR ECONOMIC CO - OPERATION AND DEVELOPMENT

(OECD) Guidande n. 129, Guidance document on using cytotoxicity tests to estimate starting doses for acute oral systemic toxicity tests, 2010.Disponível em:

<<http://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=env/jm/mo no%282010%2920&doclanguage=en>. Acesso em: 19 abr. 2016.

ORGANIZATION FOR ECONOMIC CO - OPERATION AND DEVELOPMENT (OECD), 2017. OECD QSAR Toolbox v. 3.2 How to use the Toolbox AOP workflow for Skin Sensitization. Disponível em:< http://www.oecd.org/chemicalsafety/risk- assessment/theoecdqsartoolbox.htm > Acesso em: 19 jan. 2017.

PARANÁ. Lei 18.603, e 22 de dezembro de 2015. Proíbe a utilização de animais para desenvolvimento de experimentos e testes de produtos cosméticos, de higiene pessoal, perfumes, e seus componentes. Diário Oficial do Poder Executivo.

Disponível em:

<https://www.documentos.dioe.pr.gov.br/dioe/consultaPublicaPDF.do?action=pgLoc alizar&enviado=true&numero=9603&dataInicialEntrada=&dataFinalEntrada=&search =teste+em+animais&diarioCodigo=3&submit=Localizar&localizador=>. Acesso em: 30 jan. 2017.

PATHAK MA. FITZPATRICK TB. Preventive treatment of sunburn, dermatoheliosis and skin cancer with sun-protective agents. In FITZPATRICK TB. et al. 4th ed. Dermatology in General Medicine, New York: McGraw-Hill, 1993. Cap.137, p. 1689- 1716.

PENG, L-C. et al. Optimization of water-in-oil nanoemulsions by mixed surfactants. Colloids and Surfaces A: Physicochemical and Engineering Aspects,v. 370, p. 136– 142, 2010.

PESHKOVSKY, A. S.; PESHKOVSKY, S. L.; BYSTRYAK, S. Scalable high-power ultrasonic technology for the production of translucent nanoemulsions. Chemical Engineering and Processing, v. 69, p. 77– 82, 2013.

POOTONGKAM, S.; NEDOROST, S. Oat and Wheat as Contact Allergens in Personal Care Products. Dermatitis, v. 24, n. 5, p. 291-95, 2013.

PRIETO, P.; PINEDA, M.; AGUILAR, M. Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Analytical Biochemistry, v. 269, p. 337–341, 1999.

PIZARRO, A. P. B. et al. O aproveitamento do resíduo da indústria do sisal no controle de larvas de mosquitos. Revista da Sociedade Brasileira de Medicina Tropical, v. 32, n. 1, p. 23-29, 1999.

PONS-GUIRAUD, A. Dry skin in dermatology: a complex physiopathology, Journal of the European Academy of Dermatology and Venereology, v. 21 (SUPPL. 2), p. 1- 4, 2007.

RAMOS-E-SILVA, M.; JACQUES, C. M-C. Epidermal barrier function and systemic diseases. Clinics in Dermatology, v. 30, p. 277–279, 2012.

RIBEIRO, C. Cosmetologia aplicada a dermatoestética. 2 ed. São Paulo: Pharmabooks, 2010. 441p.

RIBEIRO, B. D. et al. Functional properties of saponins from sisal (Agave sisalana) and juá (Ziziphus joazeiro): Critical micellar concentration, antioxidant

andantimicrobial activities. Colloids and Surfaces A: Physicochem. Eng. Aspects, v. 436, p. 736– 743, 2013.

RIBEIRO, B. D.; BARRETO, D.W.; COELHO, M. A. Z. Use of micellar extraction and cloud point preconcentration for valorization of saponins from sisal (Agave sisalana) waste. Food and Bioproducts Processing, v. 94, p. 601-609, 2015.

RIBEIRO, R. C. A. et al. Production and Characterization of Cosmetic

Nanoemulsions Containing Opuntia ficus-indica (L.) Mill Extract as Moisturizing Agent. Molecules, v. 20, p. 2492-2509, 2015.

ROCHA-FILHO P. A. et al. Nanoemulsions as a vehicle for drugs and cosmetics. Nanoscience & Technology, v. 1. n. 1, p. 2-5, 2014.

RODRIGUES, R. O. Desenvolvimento de emulsões contend extrato de Kalanchoe brasiliensis Cambess e avaliação clínica da eficácia hidratante. 2016. 88f.

Dissertação (Mestrado em Ciências Farmacêuticas) – Universidade Federal do Rio Grande do Norte, Natal, 2016.

SABERI, A. H.; FANG, Y.; McCLEMENTS, D. J. Fabrication of vitamin E-enriched nanoemulsions: Factors affecting particle size using spontaneous emulsification. Journal of Colloid and Interface Science, v. 391, p. 95-102, 2013.

SANTOS, J. D. G. et al. Antimicrobial activity of Agave sisalana. African Journal of Biotechnology, v. 8, n. 22, p. 6181-84, 2009.

SANTOS, J. D. G. et al. Aqueous extraction of pectin from sisal waste Carbohydrate Polymers, v. 92, n. 2, p. 1997-2001, 2013.

SANTOS-ZEA L. et al. Characterization of concentrated agave saps and storage effects on browning, antioxidant capacity and amino acid content. Journal of Food Composition and Analysis, v. 45, p. 113–120, 2016.

SÃO PAULO. Lei 15.316, de 23 de janeiro de 2014. Proíbe a utilização de animais para desenvolvimento, experimento e teste de produtos cosméticos e de higiene pessoal, perfumes e seus componentes e dá outras providências. Diário Oficial do

Poder Executivo. Disponível em:

<http://dobuscadireta.imprensaoficial.com.br/default.aspx?DataPublicacao=2014012 4&Caderno=DOE-I&NumeroPagina=1>. Acesso em: 30 jan. 2017.

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