Chapitre 7 : Discussion et conclusion
3. Conclusion
Bref, cette étude démontre que la composante inflammatoire est importante pour qu’une perte de réactivité du système respiratoire soit observée. Effectivement 1 semaine après la dernière sensibilisation au HDM, les souris Cd34-/- ont une réactivité similaire aux souris
wt. Ce phénotype est très intrigant et nécessite plus de compréhension. Ce mémoire démontre aussi qu’il est important de considérer l’implication des éléments non-contractiles à la réactivité système respiratoire et non seulement la participation d’éléments contractiles (contraction du muscle lisse). L’environnement du poumon différent en absence ou présence de l’expression du CD34 par certaines cellules pourrait effectivement influencer d’avantage la réactivité du système respiratoire. Il serait donc essentiel d’ajouter une expérience de souris chimères afin de déterminer le rôle de l’expression du CD34 sur les différentes composantes du poumon en contexte d’asthme allergique soit : les cellules
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hématopoïétiques et les cellules résidentes du poumon (endothéliales). Des transferts de cellules issues de la moelle osseuse de souris wt ou Cd34-/- seront injectées par voie
intraveineuse dans des souris wt et Cd34-/- (vice versa) afin de déterminer l’implication des
différentes cellules au phénotype de perte de réactivité du système respiratoire. Cette expérience ajoutera à l’étude une grande valeur scientifique et permettra d’orienter les expérimentations futures.
En conclusion, le CD34 est un joueur important dans l’asthme. Bien que notre étude ait démontré un rôle important pour cette molécule dans la réactivité du système respiratoire dans l’asthme, plusieurs études devront être réalisées afin de complètement élucider les mécanismes d’action de cette molécule dans ce désordre pulmonaire.
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