De acordo com os resultados obtidos no presente estudo, podemos concluir que:
• A ooforectomia contribui para um aumento de peso em ratas de laboratório mesmo com um treinamento físico com intensidade submáxima de 60%, com duração de 3 meses.
• O treinamento físico com intensidade submáxima de 60%, com duração de 3 meses é capaz de melhorar o desempenho físico de ratas ooforectomizadas.
• A cartilagem articular dos côndilos medial e lateral da tíbia respondem de maneira diferente à ooforectomia e à atividade física.
• A deprivação de estrógenos provocou um aumento da espessura da zona superficial da cartilagem articular do côndilo medial da tíbia, também observada com o treinamento, porém em menor grau.
• A ooforectomia produziu um aumento relativo da densidade numérica dos condrócitos na zona profunda da cartilagem articular do côndilo medial da tíbia. O exercício associado com a ooforectomia não produziu este efeito.
• A deprivação de estrógenos produziu uma diminuição do volume dos núcleos dos condrócitos das zonas superficial e média da cartilagem articular dos côndilos lateral e medial. O exercício também não influenciou este dado.
• A ooforectomia não produziu efeito no volume nuclear dos condrócitos da cartilagem. Porém o exercício provocou uma diminuição do volume nuclear dos condrócitos da zona profunda da cartilagem articular do côndilo lateral da tíbia. • Tanto a ooforectomia isoladamente como em conjunto com a atividade física
provocaram um aumento da produção de colágeno na matriz da cartilagem.
• Tanto a ooforectomia isoladamente, quanto a ooforectomia associada à atividade física, causam lesões semelhantes na cartilagem articular da epífise proximal da tíbia.
78
REFERÊNCIAS
ANDERSON, C. E. The structure and function of cartilage. Journal of Bone and Joint Surgery, v. 44A, p. 77-786, 1962.
ANDRIACCHI, T. P.; MÜNDERMANN, A. The role of ambulatory mechanics in the initiation and progression of knee osteoarthritis. Curr Opin Rheumatol, v. 18, n. 5, p. 514- 518, 2006.
AROKOSKI, J.; KIVIRANTA, L.; JURVELIN, J.; TAMMI, M. E.; HELMINEN, H. J. Long- distance running causes site-dependent decrease of cartilage glycosaminoglycan content in the knee joints of beagles dogs. Arthritis Rheum, v. 36, n. 10, p. 1451-1459, 1993.
AROKOSKI, J.; HYTTINEN, M.; LAPVETELAINEN, T.; TAKACS, P.; KOSZTACZKY, B; MOIS, L.; KOVANEN, V.; HELMINEN, H. Decrease birefringence of the superficial zone collagen network in the canine knee (stifle) articular cartilage after long distance running training, detected by quantitative polarized light microscopy. Ann Rheum Dis., v. 55, n. 4, p. 253-264 1996.
ASIKAINEN, T. M.; KUKKONEN-HARJULA, K.; MIILUNPALO, S. Exercise for health for early postmenopausal women: a systematic review of randomised controlled trials. Sports Med., v. 34, n.11, p. 753-778, 2004.
ATRA, E. Artrose, reumatologia prática. Rio de Janeiro: Eleá CiênciaEditorial: 1995, p.58- 63.
BIHARI-VARGA, M.; FRAKAS, T.; BIRÓ, T. Changes in the cartilage proteoglycans in relation to age and osteoarthrosis. Acta Biol Hung., v. 35, p. 325-331, 1984.
BLOEBAUM , W. The morphology of the surface of artcular cartilage in adult rats. J Anat, v. 131, p. 333-346, 1980. (Supplement, 2).
BOGOSLAVSKY, A. Alterações da cartilagem hialina da cabeça do fêmur de ratos Wistar corredores, em função do envelhecimento. 2006. 96 f. – Dissertação (Mestrado) - Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, 2006. BRANDAN, E. Proteoglycans in skeletal muscle. Brazilian Journal of Medical and
Biological Research, v.27, p.109-16, 1994.
BRÜEL, A.; OXLUND, H. Growth hormone influences the conten and composition of collagen in the aorta from old rats. Meach Ageing Dev., v. 123, n. 6, p. 627-635, 2002.
BRUYERE, O.; GENANT, H.; KOTHARI, M.; ZAIM, S.; WHITE, D. Longitudinal study of magnetic resonance imaging and standard X-rays to assess disease progression in
osteoarthritis. Osteoarthritis Cartilage, v. 5 n. 1, p. 98-103, 2007.
BUCKWALTER, J. A. Osteoarthritis and articular cartilage use, disuse, and abuse: experimental studies. J Rheumv., v. 22, p. 13-15, 1995. (Supplement, 43).
BUCKWALTER, J. A.; MANKIN, H. J. Articular cartilage: tissue design and chondrocyte- matrix interactions. Instr Course Lect., v. 47, p. 477-486, 1998.
BURSTEIN D.; BASHIR, A.E.; GRAY, M.L. MRI techniques in early stages of cartilage disease. Investigative Radiology, v.35, n.10, p.622-638, 2000.
BUSH, P.G.; HODKINSON, P.D.; HAMILTON, G.L.; HALL, A.C. Viability and volume of in situ bovine articular chondrocytes-changes following a single impact and effects of
medium osmolarity. Osteoarthritis Cartilage, v. 13, n.1, p.54-65, 2005.
CHRISTGAU, S.; GARNERO, P.; FLEDELIUS, C.; MONIZ, C.; ENSIG, M. Collagen type II C-telopeptide fragments as an index of cartilage degradation. Bon, v. 29, p. 209-215, 2001. CHRISTGAU, S.; TANKÓ, L.; CLOOS, P. A. C.; MOURITZEN, U.; CHRISTIANSEN, C. M. D.; DELAISSÉ, J. M.; HOEGH-ANDERSON, P. Suppression of elevated cartilage turnover in postmenopausal women and ovariectomized rats by estrogen and selective estrogen receptor modulator (SERM). Menopause, v. 11, n. 5, p. 508-518, 2004.
CORVOL, M. T. The chondrocyte: from cell aging to osteoarthritis. Joint Bone Spine, v. 67, p. 557-560, 2000.
CULAV, E. M.; CLARK, C. H.; MERRILEES, M. J. Connective Tissues: Matrix
composition and its relevance to physical therapy. Physical Therapy, v. 79, n. 3, p. 308-319, 1999.
DEGROOT, J.; VERZIJ, N.; BANK R.A.; LAFEBER, F.P.J.G.; BIJLMSA, J.W.J.; TEKOPPELE, J.M. Age-related decrease in proteoglycan synthesis of human articular chondrocytes. Arthr. Rheum, v.19, n.1, p.87-93, 1999.
DING, C.; CICUTTINI, F.; SCOTT, F.; GLISSON, M.; JONES, G. Sex differences in knee cartilage volume in adults: role of body and bone size, age and physical activity.
Rheumatology, v. 42, p.1317-1323, 2003.
ECKSTEIN, F.; HUDELMAIER, M.; PUTZ, R. The effects of exercise on human articular cartilage. J.Anat., v. 208, p.491-512, 2006.
ECKSTEIN, F.; TIESCHKY, M.; FABER, S.; ENGLMEIER, K. H.; REISER, M. Functional analysis of articular cartilage deformation, recovery, and fluid flow following dynamic exercise in vivo. Anat Embryol., v. 200, p. 419-424, 1999.
80
EGRI, D.; BATTISTELLA, L.R.; YOSHIARI, N.H. Envelhecimento da cartilagem articular. Revista Brasileira de Reumatologia, v.39, n.1, p.45-48, 1999.
FETTER, N. L.; LEDDY, H. A.; GUILAK, F.; NUNLEY, J. A. Composition and transport properties of human ankle and knee cartilage. J Orthop Res, v. 24, n. 2, p. 211-219, 2006.
FITZGERALD, G.K.; CHILDS, J.D.; RIDGE, T.M.; IRRGANG, J.J.Agility and perturbation training for a physically active individual with knee osteoarthritis.Phys Ther, v.82, p.4, p.372- 82, 2002.
FLECKNELL, P. Pain - assessment, alleviation and avoidance in laboratory animals. ANZCCART News, v.12, n. 4, p. 1-10,1999.
GARDNER, D. L.; MCGILLIVRAY, D. C. Surface structure of articular cartilage. Annals of Rheumatic Disease, v. 30, p. 10-14, 1971.
GARNERO, P.; PIPERNO, M.; GINEYTS, E.; CHRISTGAU, S.; DELMAS, P. D.; VIGNON, E.Cross sectional evaluation of biochemical markers of bone, cartilage, and synovial tissue metabolism in patients with knee osteoarthritis: relations with disease activity and joint damage. Ann Rheum Dis., v. 60, p. 619-626, 2001.
GENAZZANI, A. R.; GAMBACCIANI, M. Effect of climacteric transition and hormone replacement therapy on body weight and body fat distribution. Gynecol Endocrinol., v. 22, n. 3, p. 145-150, 2006.
GRODZINSKY, A.J.; Eletromechanical and physiochemical properties of connective tissue. CRC Critical Reviews in Biomedical Engineering, v.9, n.2, p.133-9, 1983.
GULISANO, M.; DELRIO, A.N.; FADDA, M.; MARCED, D.U.S. Human articular cartilage during osteoarthrosis: a study under the scanning electron microscope.Ital J Anat Embryol, v.98, n.3, p.175-85, 1993.
HAAPALA, J.; AROKOSKI, J.P.A. HYTTINEN, M.K.; LAMMI, M.; TAMMI, M.; KOVANEN, V.; HELMINEN, H.J.; KIVIRANTA, I. Remobilization does not fully restore immobilization induced articular cartilage atrophy. Clin. Otrthop. Rel. Res, v.362, p.218- 229, 1999.
HALL, A. C.; URBAN, J. P. G.; GEHL, K. A. Effects of compression on the loss of newly synthetized proteoglycans and proteins from cartilage explants. Arch Biochem Biophys, v. 286, p. 20-29, 1991.
HARDINGHAM, T.; FOSANG, A. J. Proteoglycans: many forms and many functions. FASEB J, v. 6, n. 3. p. 861-870, 1992.
HARDINGHAM, T.; RAYAN, V.; LEWTHWAITE, J. C. Regulation of cartilage matrix synthesis by chondrocytes. Rev Rhum Ed Fr., v. 61, n. 9, p. 93-98, 1994(Supplement, 2).
HARDINGHAM, T.; TEW, S.; MURDOCH, A. Tissue engineering: chondrocytes and cartilage. Arthritis Res, v. 4, n. 3, p. 63-68, 2002.
HASCALL, V.C.; KIMURA, J.H.; Proteoglycans: isolation and characterization. Methods in Enzymology, 82,p.769-800 1982, Pt A.
HEISE, N.; TOLEDO, O.M.S. Age-related in glycosaminoglycan distribution in dfferent anatomical sites on the surface of knee-joint articular cartilage in young rabbits. Anals of Anaomy, v.175, n.1, p.35-40, 1993.
HERZOG, W.; DIEL, S.; SUTER, E.; MAYZUS, P. LEONARD T.; MULLER, C.; WU, J. Z. Material and functional properties of articular cartilage and patellofemoral contact mechanics in an experimental model of osteoarthritis. J Biomech., v. 31, n. 12, p. 1137-1175, 1998. HOEG-ANDERSEN, P.; TANKO, L. B.; ANDERSEN, T. L.; LUNDBERG, C. V.; MO, J. A.; HEEGAARD, A. M.; DELAISSE, J. M.; CHRISTGAU, S. Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application. Arthritis Rest Her., v. 6, n. 2, p. 169-180, 2004.
HUBER, M.; TRATTNIG, S.; LINTNER, F. Anatomy, biochemistry and physiology of articular cartilage. Invest Radiol., v. 35, n. 10, p. 573-580, 2000.
HUDELMAIER, M.; GLASER, C.; ENGLMEIER, K.H.; REISER, M.; PUTZ, R.;
ECKSTEIN, F. Age-related changes in the morphology on deformational behavior of knee joint cartilage. Arthritis and Rheumatism, v.44, n.11, p.2556-2561, 2001.
INGELMARK, B. E. Morpho-physyological aspects of gymnastic exercise. Federation Internationale de I’Education Phydique Bullettin, v. 27, p. 37-41, 1957.
IRWIN, M. L.; YASUI, Y.; ULRICH, C. M.; BOWEN, D.; RUDOLPH, R. E.; SCHWARTZ, R. S.; YUKAWA, M.; AIELLO, E.; POTTER, J. D.; MCTIERNAN, A. Effect of exercise on total and intra-abdominal body fat in postmenopausal women: a randomized controlled trial. JAMA, v. 289, n. 3, p. 323-330, 2003.
JONES, O.; GLISSON, M.; HYNES, K.; CICUTTINI, F. Sex and site diferences in cartilage development – a possible explanation for variations in knee osteoarthritis in later life. Arthritis Rheum., v. 43, n. 11, p. 2543-2549, 2000.
JUNQUEIRA, L. C. U.; BIGNOLAS, G.; BRENTANI, R. R. Picrossirius red staining plus polarization microscopy, a specific method for collagen detection in tissue sections.
Histochem J., v. 11, p. 447-455, 1979.
KAAB, M. J.; ITO, K.; CLARK, J. M.; NOTZLI, H. P. Deformation of articular cartilage collagen structure under static and cyclic loading. J Orthop Res., v. 16, n. 6, p. 743-751, 1998.
82
KARVONEN, R. L.; NEGENDANK, W. G.; TEIGE, R. A.; REED, R. A.; MILLER, P. R.; FERNANDEZ, F. Factors affecting articular cartilage thickness in osteoarthritis and aging. J Rheumatol., v. 21, n. 7, p. 1310-1318, 1994.
KIM, Y. J.; SAH, R. L.; GRODZINSKY, A. J.; PLAAS, A. H. K.; SAND, Y. J. D.
Mechanical regulation of cartilage biosynthetíc behavior: Physical stimuli. Arch Biochem Biophys., v. 311, n. 1, p. 1-12, 1994.
KIM, H. A.; SUH, D.; SONG, Y. W. Relationship between chondrocyte apoptosis and matrix depletion in human articular cartilage. J Rheum., v. 28, n. 9, p. 2038-2045, 2001.
KIVIRANTA, I.; JURVELIN, J.; TAMMI, M.; SÄÄMÄNEN, A. M.; HELMINEN, J. H. Weight bearing controls glycosaminoglycan concentration and articular cartilage thickness in the knee joints of young beagle dogs. Arthritis Rheum, v. 7, n. 30, p. 801-809, 1987.
KIVIRANTA, I.; TAMMI, M.; JURVELIN, J.; SÄÄMÄNEN, A. M.; HEIMINEN, J. H. Moderate running exercise augments glycosaminoglycans and thickness of articular cartilage in the joint of young beagle dogs. J Orthop Res., v. 6, n. 2, p. 188-195, 1988.
KUETTNER, K. E.; AYDELOTTE, M. B.; THONAR, E. J. Articular cartilage matrix and structure: a minireview. J Rheumatol Suppl, v. 27, p. 46-48, 1991.
LANE, N. E.; BUCKWALTER, J.A. Exercise: a cause of osteoarthritis? Rheum Dis ClinNorth Am, v. 19, n. 3, p. 617-33, 1993.
LEANNE, S.; CAROLINE, F.; SHONA, B. Sports participation, sports injuries an osteoarthritis. Sports Med., v. 28, n. 2, p. 123-135, 1999.
LEVANON, D. STEIN.;H. The articular cartilage of the rabbit knee: A scanning electron microscopy study. Cells and Materials, v.1, p.219-229, 1991.
LOESER, R.F., Aging and the etiopathogenesis and treatment of osteoarthritis. Rheumatic Diseases Clinics of North America, v.26, n.3, p.547-567, 2000.
LOTHE, K.; SPYCHER, M. A.; RUTTNER, J. R. Human articular cartilage in relation to age, A morphometric study. Exp Cell Biol., v. 47, n. 1, p. 22-28, 1979.
MAFFULLI, N.; KING, J. B. Effects of physical activity on some components of skelectal system. Sports Medicine, v. 13, n. 6, p. 393-407, 1992.
MARCONDES, F. K.; BIANCHI, F. J.; TANNO, A. P. Determination of the estrous cycle phases of rats: some helpful considerations. Braz. J. Biol., v. 62. n. 4A, p. 609-614, 2002. MAROUDAS, A. Balance between swelling pressure and collagen tension in normal and degenerative cartilage. Nature, v.260, n.5554, p.808-809, 1976.
MARTI, T.; KNOBLOCH, M.; TSCHOPP, A.; JUCKER, A.; HOWALD, H. Is excessive running predictive of degenerative hip disease? Controlled study of former elite athletes. Brsitish Medical Journal, v. 299, p. 91-93, 1989.
MARTINS, R. R.; PEREIRA, N. M. L. SILVA, T. M. A. Liquid-base cytology: a new method for estral cycle study in wistar's rats, Acta Cir. Brás., v. 20, n. 1, p. 46-49, 2005. MCDEVITT, C.A.; MUIR, H. Biochemical changes in the cartilage of the knee in
experimental and natural osteoarthritis in the dog. Journal of Anatomy,v.58, n.1, p,94-101, 1976.
MERCURO, G.; SAIU, F.; DEIDDA, M.; MERCURO, S.; VITALE, C.; ROSANO, G. M. Impairment of physical exercise capacity in healthy postmenopausal women. Am Heart J., v. 151, n. 4, p. 923-927, 2006.
MOLLENHAUER, J.; BEE, J. A.; LIZARBE, M. A. VON DER MARK, K. Role of anchorin CII, a 31,000-mol wt membrane protein, in the interaction of chondrocytes with type II collagen. J Cell Biol., v. 98, p. 1572-1579, 1984.
MOURITZEN, U.; CHRISTGAU, S.; LEHMANN, H. J.; TANKO, L. B.; CHRISTIANSEN, C. Cartilage turnover assessed with a newly developed assay measuring collagen type II degradation products: influence of age, sex, menopause, hormone replacent therapy and body mass index. Ann Rheum Dis., v. 62, p. 332-336, 2003.
NEWTON, P. M.; MOW, V. O.; GARDNER, T. R.; BUCWALTER, L. A.; ALBRIGHT, J. P. The effect of Iifelong exercise on canine articular cartilage. Am J Sports Méd., v. 25, n. 3, p. 282-287, 1997.
NISHIMURA, T.; HATTORI, A.; TAKAHASHI, H. Arrangement and identification of proteoglycans in basement membrane and intramuscular connective tissue of bovine semitendinosus muscle. Acta Anatomica, v.155, p.257-265, 1996.
O’CONNOR, P.; ORFORD, C.E.; GARDNER, D.L. Differential response to compressive loads of zones of canine hyaline articular cartilage: micromechanical, light and electron microscopic studies. Annals of the Rheumatic Diseases, v. 47, n.5, p.414-420, 1988. ODA, J. Y. Estudo Morfoquantitativo das alterações decorrentes do envelhecimento na cartilagem articular da epífise distal do fêmur de ratos. 2005. 77 f. Dissertação (Mestrado) - Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2005.
OTTERNESS, I. G.; ESKRA, J. D.; BLIVEN, M. L.; SHAY, A. K.; PELLETIER, J. P.; MILICI, A. J. Exercise protects against articular cartilage degeneration in the hamster. Arthritis Rheum, v. 41, n. 11, p. 2068-2076, 1998.
PANULA, H. E.; HYTTINEN, M. M.; AROKOSKI, J. P. A.; LANGSJO, T. K.; PELTTARI, A.; KIVIRANTA, I.; HELMINEN, H. Articular cartilage superficial zone collagen
84
birefringence reduced and cartilage thickness increased before surface fibrillation in experimental ostoarthritis. Ann Rheum Dis., n. 57, p. 237-245 1998.
PALMOSKI, M.J.; COLYER, R.A.; BRANDT, K.D. Joint motion in the absence of normal loading does not maintain normal cartilage. Arthritis and Rheumatism, v.23, n.3, p.325- 334, 1980.
PALMOSKI, M.J.; BRANDT, K.D. Running inhibits the reversal of atrophic changes in canine knee cartilage after removal of leg cast. Arthritis an Rheumatism, n.11, p.1329-37, 1981.
PAP, G.; EBERHARD, T. R.; STÜRMER, I.; MACHNER, A.; SCHWARTZBERG, H.; ROESSNER, A.; NEUMANN, W. Development of osteoarthritis in the knee joints of Wistar rats after strenous running exercise in a running wheel by intracranial self-stimulation. Path Res Pract., v. 194, p. 41-47, 1998.
PARKER, D.; HWA, S. Y.; SAMBROOK, P.; GHOSH, P. Estrogen replacement therapy mitigates the loss of joint cartilage proteoglycans and bone mineral density induced by ovariectomy and osteoarthritis. APLAR Journal of Rheumatology, v. 6, p. 116-127, 2003. POOLE, A. R.; KOJIMA, T.; YASUDA, T.; MWALE, F.; KOBAYASHI, M.; LAVERTY, S. Composition and structure of articular cartilage: a template for tissue repair. Clin Orthop Relat Res., v. 391, p. 26-33, 2001.
PRITZKER, K. P. Animal models for osteoarthritis processes, problems and prospects. Ann Rheumatic Dis., v. 53, p. 406-420, 1994.
RASCH,.J. Cinesiologia e anatomia aplicada. In:___ RASCH, P.J. Cinesiologia, 7ª. Ed. Rio de Janeiro: Guanabara Koogan, 1991, p.12-21.
REDLER, I; ZIMNY, M.L. Scanning electron microscopy of normal and abnormal articular cartilage and synovium. J Bone Joint Surg Am, v. 52, n.7, p.1395-404, 1970.
ROUGHLEY, P..; WHITE, R. J. Age-related in the structure of proteoglycan subunits from human articular cartilage. The Journal of Biological hemistry, v.225, n.1, p. 217-224, 1980. ROUGHLEY, P.J. Age-associated changes in cartilage matrix – implications for tissue repair. Clinical Orthopaedics and Related Research, v.391, s.153-160, 2001.
RUOSLAHTI, E.; YAMAGUCI, Y.C. Proteoglycans as modulators of growth factor activities. Cell, v.64, n.5, p.867-869, 1991.
SÄÄMÄMEN, A.; TAMMI, M.; KIVIRANTA, I.; JURVELIN, J.; HELMINEN, H.J. Running exercise as a modulator of proteoglycans matrix in the articular cartilage of young rabbits. International Journal of Sports Medicine, v.9, n.2, p.127-132, 1988.
SÄÄMÄMEN, A.; KIVIRANTA, I.; JURVELIN, J.; HELMINEN, H. J.; TAMMI, M.
Proteoglycan and collagen alterations in canine knee articular cartilage following 20 km daily running exercise for 15 weeks. Conn Tis Res., v. 30, p. 191-201, 1994.
SALVATORE, C. A.; SCHREIDER, G. Pesquisas cariométricas no ciclo estral e gravídico. Mem Inst Butantã, v. 20, p. 39-78, 1947.
SCHMIDT, MB.; MOW, V.C.; CHUN, L.E.; EYRE, D.R. Effects of proteoglycan extraction on the tensile behavior of articular cartilage. Journal of Orthopaedic Research, v.8, n.3, p.353-363, 1990.
SILVA, G. J. J.; BRUM, P. C.; NEGRÃO, C. E.; KRIGER, E. M. Acute and Chronic Effects of Exercise on Baroreflexes in Spontaneoously Hypertensive Rats. Hipertesion, v. 30, p. 714- 726, 1997.
SOMMER, H. M. The biochemical and metabolic effect os a running regime on the Achilles tendon in the rat. Orthopaedics, v.11, p. 71-75, 1987.
STEVENS, A.; LOWE, J. J. Tecido Conjuntivo In:____ STEVENS, A.; LOWE, J. Human Histology, 2ª.ed. USA: Morby, 1997, p.49-64.
STOCKWELL, R. A. Lipid content of human costal and articular cartilage. Ann Rheum Dis., v.26, n. 6, p. 481-486, 1967.
TAKANO, H.; AIZAWA, T.; IRIE, T.; KOKUBUN, S.; ITOI, E. Estrogen deficiency leads to decrease in chondrocyte numbers in the rabbit growth plate. J Orthop Sci., v. 2, n. 4, p. 366- 374, 2007.
TALWAR, R. M.; WONG, B. S.; SVOBODA, K.; HARPER, R. P. Effects of estrogen on chondrocyte proliferation and collagen synthesis in skeletally mature articular cartilage. J Oral Maxillofac Surg., v. 64, n. 4, p. 600-609, 2006.
TAN, A.H.; MITRA, A.K.; CHANG, P.C.; TAY, B.K.; NAG, H.L.; SIM, C.S.Assessment of blood-induced cartilage damage in rabbit knees using scanning electron microscopy. J Orthop Surg, v.12, n.2, p.199-204, 2004.
TESCHE, F.; MIOSGE, N. New aspects of the pathogenesis of osteoarthritis: the role of fibroblast-like chondrocytes in late stages of the disease. Histol Histopathol., v. 20, n. 1,p. 329-337, 2005.
THORNDIKE, E. A.; TURNER, A. S. In search of an animal model for postmenopausal diseases. Front Biosci., v. 3c, p. 17-26, 1998.
TRATTNIG S. Overuse of hyaline cartilage and imaging. Euopean Journal of Radiology, v.25, n.3, p.88-198, 1997.
86
TUFFERY, A. A. Anaesthesia. In: _____Laboratory animals. An introduction for experiments. 2. ed. UK: John Wiley & Sons Ltd, 1995, p. 324.
VAN DEN HOOGEN, B.M.; VAN DE LEST, C.H.A.; VAN WEEREN, P.R.; LAFEBER, F.P.J.G.; LOPES-CARDOZO, M,; VAN GOLDE, L.M.G. Loading-induced changes in synovial joints affect cartilage metabolism. British Journal of Rheumatology, v.37, n.6, p.671-676, 1998.
VAN KUPPEVELT, H.M.S.M.; DOMEN, J.G.W.; CREMERS, F.P.M.; KUYPER, C.M.A. Staining of proteoglycans in mouse lung alveoli. Ultrastructural localization of anionic sites. The Histochemical Journal, v.16, n.6, p.657-669, 1984.
WALKER, J.M. Epidemiology and economics of arthritis. In:____WALKER, J>M.; HELEWA, R. Physical Therapy in Arthritis. USA: WB. Saunders Company, 1996.
WARD, D.J.; TIDSWELL, M.E. Osteoartrite. In:____ DOWNIE, P.; CASH, L. Fisioterapia em Ortopedia e Reumatologia, São Paulo: Panamericana, 1987; p.263-279.
WIGHT, T.N. HEINEGARD, D.K.; HASCALL,V.C. Proteoglycans :Structure and functions, In: ____HAY, E. Cell Biology of Extracellular Matrix. 2ª.ed. New York: Plenum Press, 1991.
WLUKA, A. E.; WOLFE, R.; DAVID, S. R.; STUCKEY, S.; CICUTTINI, F. M. Tibial cartilage volume change in healthy postmenopausal women: a longitunal study. Ann Rheum Dis, v. 63, p. 444-449, 2004.
WU, J. Z.; HERZOG, W. Elastic anisotropy of articular cartilage is associated with the microestrutures of collagen fibers and chondrocytes. J Biomech., v. 35, n. 7, p. 931-942, 2002.
WULFSOHN, D.; GUNDERSE, H. J.; VEDEL JENSE, E. B.; NYENGAARD, J. R. Volume estimation from projections. J Microsc., v. 215, p. 111-120, 2004. (Supplement, 2).
YANAGISHITA, M. Function of proteoglycans in the extracellular matrix. Acta Pathologica Japonica, v.43, n.6, p.283-93, 1993.
YASUNORI, S.; NORIYUKI, S.; EICHI, T.; KAZUHARU, I.; TOSHIHIKO, Y. Effects of running exercise on the mandible and tíbia of ovariectomized rats. J Bone Miner Metab., v. 19, p. 159-167, 2001.