In vivo gene electrotransfer
In vivo Gene electrotransfer: optimisation and mechanisms.
... of in vivo delivery of short high voltage pulses, like in vivo DNA electrotransfer, also termed electrogenetherapy, are rapidly developing ...efficient in vivo gene ...
315
Optical imaging of luminescence for in vivo quantification of gene electrotransfer in mouse muscle and knee.
... luciferase in serum At different times after electrotransfer, blood samples were collected by retro orbital puncture of anaesthetized mice, centrifuged at 3000 rpm for 10 minutes at 4°C, and serums were ...
16
Electroporation-Induced Stress Response and Its Effect on Gene Electrotransfer Efficacy: In Vivo Imaging and Numerical Modeling
... increased in order to reduce the effect of boundary on simulation ...drop in resistance occurs due to formation of local conductive pathways [34], which were introduced in the model as small ...
14
Direct visualization at the single-cell level of siRNA electrotransfer into cancer cells
... interference-mediated gene silencing approach is pro- mising for therapies based on the targeted inhibition of disease- relevant ...cells in vitro and in ...effective in the field of ...
6
Safe and efficient novel approach for non-invasive gene electrotransfer to skin
... help in the eradication of primary tumors and/or metastasis is of great ...response. In order to determine the efficiency of in vivo pIL-12 gene-electrotransfer (pIL-12 GET), 25 ...
14
Electrotransfer of CpG free plasmids enhances gene expression in skin
... motifs in plasmids reduce gene ...quoted in our contribution was ...long-term gene expression in vivo, Mol Ther, 5 (2002) ...illustrated in a previous paper of the same ...
37
Noninvasive Gene Electrotransfer in Skin
... for gene therapy and ...injected in the ...expression. Gene electrotransfer (GET) is one of the most effective ...by in vivo fluorescence imaging during more than 2 weeks as the ...
21
Gene Electrotransfer in 3D Reconstructed Human Dermal Tissue
... Current Gene Therapy, 2015, Vol. 0, No. 0 Madi et al. interactions in a healthy ...collagen in tumor tissue ...electrotransfected in vivo than solid tumors with a rich extracellular ...
6
Efficiency of high- and low-voltage pulse combinations for gene electrotransfer in muscle, liver, tumor, and skin.
... efficient gene electrotransfer in the tissues explored: muscle, tumor, liver, and skin (Table ...listed in Table 1 are based on the results presented here and are limited to an experimental ...
14
An in vivo reporter assay for sRNA-directed gene control in Gram-positive bacteria: identifying a novel sRNA target in Staphylococcus aureus
... system in Staphylococcus aureus cells. a, Gene names refer to the an- notations in the ...nucleotides in the 5 UTR of the cloned mRNA ...fused in-frame to ...obtained in the ...
15
Gene Expression of Mouse Hippocampal Stem Cells Grown in a Galactose-Derived Molecular Gel Compared to In Vivo and Neurospheres
... ex vivo for several days on a supramolecular low molecular weight hydrogel based on a single small molecule, N-heptyl-D-galactonamide ...maintained in culture ...differences in transcripts of genes ...
17
Ex Vivo Selection of Transduced Hematopoietic Stem Cells for Gene Therapy of β-Hemoglobinopathies
... conducted in ongoing clinical trials. 2,5 The protocol described in this manuscript ensures efficient selection, ensures high recovery rate of transduced hematopoietic stem/progen- itor cells after only 24 ...
44
Prédiction du passage transplacentaire in-vivo des médicaments à partir de modèles ex-vivo
... studies in this sensitive population are ...implemented in full PBPK ...Changes in renal clearance secretion were related to changes in renal plasma ...ex vivo human placenta perfusion ...
152
Generation and in vivo evaluation of IL10-treated dendritic cells in a nonhuman primate model of AAV-based gene transfer.
... imminent in rhu- mathoid arthritis patients (Queensland and New Castle Universities) or for kidney transplant in our team (Nantes ...based gene therapy emerged recently as an additional potential ...
13
Preclinical In Vivo Evaluation of the Safety of a Multi-shRNA-based Gene Therapy Against HIV-1
... analyzed in the major sites of hematopoiesis for the absolute number of human CD45 + cells ( a) and for GFP + ratio in human hematopoietic cells ( ...measured in the animals (in vivo) ...
11
Differential heat shock gene hsp70-1 response to toxicants revealed by in vivo study of lungs in transgenic mice
... phase in the lung. A specific transport process explains its toxicity in pneumocytes where paraquat elevates intracellular levels of superoxide through redox cycle (Smith ...
11
Ultrasound-activated microbubbles for tendon gene transfer: in vivo efficiency and confocal microscopy real time intracellular investigations
... ABSTRACT Ultrasound that is routinely used for imaging is now ex- ploited for therapeutic applications including drug delivery or gene transfer. Today, ultrasound imaging is an established and confident technique ...
4
Aptamer photoregulation in vivo
... aptamers in normal tissues is undesirable (12–15) because it diminishes the proportion of aptamer that targets the desired ...concentration in a pathologi- cal tissue of interest while maintaining low ...
6
L’électroporation in vivo chez l’embryon
... gène in vivo Plusieurs autres méthodes de transfert d’ADN in vivo chez le poulet, qui ont été développées plus ou moins récemment, peuvent être comparées à la technique ...succès in ...
6
Etude in vivo de l'articulation trapézométacarpienne
... Discussion Peu d’auteurs ont analysé la cinématique in vivo de sujets présentant une arthrose TM [39, 42]. Chèze et coll. utilisèrent un système d’analyse optoélectronique (Motion Analysis, Santa Rosa, ...
139