Plant and Animal Genome XXV Conference
https://pag.confex.com/pag/xxv/meetingapp.cgi/Paper/26112[07/02/2017 12:46:10]
W064: Characterization of a Large Reciprocal Chromosomal Translocation in Banana (Musa
acuminata) and its Impact on Chromosomal Segregation using NGS
ABSTRACT
Tuesday, January 17, 2017 10:50 AM - 11:10 AM
Pacific Salon 6-7 (2nd Floor)
Bananas cultivars are triploid or diploid seedless parthenocarpic clones derived from the hybridization between Musa acuminata subspecies and, for some of them, with the species M. balbisiana. Based on chromosome pairing configuration in hybrids, M. acuminata subspecies were reported to differ by a few large chromosomal structural rearrangements which may have occurred since their divergence following geographical isolation. We analyzed with DArTseq, chromosome segregation in the progenies of a seedy
M. acuminata banana accession and discovered strong segregation distortion, reduced recombination and
abnormal linkage in two regions of references chromosomes 1 and 4. Pair-end resequencing of the parental accession and comparison to the Musa acuminata references sequence, showed evidence of a heterozygous reciprocal translocation involving two distal segments of 3 Mb and 10 Mb from chromosome 1 and 4. This translocation was then confirmed through BAC-FISH and PCR experiments. The two
chromosome structures were found mutually exclusives in gametes and the rearranged structure was transmitted preferentially to the progeny. Analysis of the distribution of this distinct chromosome structures in Musa diversity allowed to make hypotheses about its origin.
Authors
Guillaume Martin
CIRAD, UMR AGAP
Françoise Carreel
CIRAD, UMR AGAP
Olivier Coriton
Institut National de la Recherche Agronomique (INRA
Catherine Hervouet
CIRAD, UMR AGAP
Céline Cardi
CIRAD, UMR AGAP
Danièle Roques
CIRAD, UMR AGAP
Mathieu Rouard
Plant and Animal Genome XXV Conference
https://pag.confex.com/pag/xxv/meetingapp.cgi/Paper/26112[07/02/2017 12:46:10]
Julie Sardos
Bioversity International
Franc-Christophe Baurens
CIRAD, UMR AGAP
Angélique D'Hont
CIRAD, UMR AGAP
Presentation - PDF file
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