of Report: Quarterly Date of

OI{CHOCENCIASIS CONIROI RNSEARCH IIEPORTIITG

SubJect:

Cytotaxonomy

of

Instltutlon:

^li{fiO/oCP

Sersormel: C.G.

^Vajime Type

of Report: Quarterly Date of

submlssi.on: I,Iarch 311

PROGRA}flM NUI,tsE1].

(oclrzn

\tE/75)

Simuliun

damnosum comp1.ex

Period.s Covered:

October

L975

- ^I{arch,

^L976

L976 Slgnature of responsible officer

aaaaaaa aaaaaaaaaaaa

1,

2,

7

).

4.

SUIl 1\[ARY

The

distribution of

S.d.amnosum r-rnd S.sirbanum

in Control

Zone

If

^has

enlarged,

as

more

areEs-E6zone EaEffi-covered.

Inadditiontotheabovespec1es9..Wo,d9.s9}!I9IEwere in

Togo

within control

zone

III.

Identiftcations of

^semples

arisj-ng from collaborative

^work

^wlth

I,III0

consultants are included.

Using the

C

banding technique,

C

banding of

centromere regi"ons ^Yras obse::rred

in Larval polytene

chrouosomes

of the

S.dannnosuxn species

group.

The

expression ot the region

was

most coEsliffiu-in g.-W,-

nosum and S.yahense.

[here

^were

no reversed banding

^seguences

with

E6@ct to-sTAfrm to ^provlde

^landmarks

for species id.entj-ficatlon.

Attenpts to find

^poJ.ytene chromosomes

ln adults

were

not suocessful.

Adults eaught bitlng

^man

at

^lcnown

locations

^and

those reared fron

pupae

frou the

^same

locations

have been preserrred

for

^taxonomio

studies.

OPDRATIOIIS DUiII}IG PERIOD UTIDER REVIDTI

Operations for the last quarter of

^{1975 ancl}

the first quarter ^of

1976

overlap

and

are therefore presentetl together in thi.s report.

FIEID

^Y/ORK

a)

toeo

Durlng the

month

of

October

Dr Davies, iir

Sowah and

I

^dtd.

a

two- clay

prospection of the Otl,

^{1.{o and}

l{ara river

systems

in norther [ogo;

In the viclnlties of lama-I(ara,

Sansanne llango and Dapango.

In

^these

hi1ly areas there

were many

stretches of rapitls ln all the rivers

but

the high water conditions probably dirl not favour the

development

of,

S.@. tte/ot'

tr

6.

Iew

Ia.rvaI

sainlrles i'iere

coilecied

froi:i'Lhe Kel:a,'/keuii cor:fIuence,

the

^Bina

or

Ako1e

river,

^{ancl -i;he}

^Balique river

near [a.r:,atougou.

A

oi

igic

speclnen vras obtaised

fron the

Kara

river at the

o1d brid.ge j::1,:;:::.-'(1'E":..

b) ^Irryffi

^yoqqs

In

^{f ctober}

^L{r.

^{0o:=3 and.}

I

r,ude

a

sunrey

of

south-easterrr ^Upper

Volta

^anrd

oolleoted

^{la:sra1 samples}

at

Yakala on

the

^White

Volta

and.

at

Ziou-Zabre on

the

^Red Vo1ta.

o)

-98e4.-e"-g9gg

0n

our retrrrn to

Oqagad.ougou,

Dr. Ie

3erre1

ltalsh

and.

I

had

a

discussion on f^urbher ^vrorlc

in ^[ogo at

^{,0 timo} when breed.ing ^v-ras

anticipated. It

^was

6ecide6

that aft:r

sampl5:r.g the Daira

river in

^Ghar:a,

I

sirould ^p;:oceed

to

Eogo

to obtajr

as nuch

material

as

pos;ible

ancl

to

^mal<e observations on

flurrial

ecological- conC.j-tions from aJ.l tiee

najo: rivers

especi-erlIy the I[onol ^ir{o and Kara.

In

lrlorrember the Dala

river

^vvl.s

visitr:d but

unsuccessfully sa;:pler.

at

^lkundipe

and Sabon-Gida.

t}:a

ruatcr

levei

^{i,'ias qui-be}

^high ai tlre tine'

^{I{oweverz i.:r}

Deoember

LIr.

Zerbo atid. Liro Av:Lssey

successiully

collected. larrra-l ^sa.mples

fron trro sites

nelrr I}ru:r).ipe"

The [o3;o

trip

r,ns ;:tar-bcf from lcirnele on i{overlbc::

J-J.

^During

^the

^course

of the

jor:-r:rey, i;wo days vrere silent

jrl

_Accr.a

at

^t]re

Un:iversity of

legon

for

cliscussione, ivlth.

3rs

Coker and [c-,itrson i','ho siud;r en4/'Ire p,rlpoorpbims

of

the

E.

-,lgg*.o5aq species

gr:oup. fle

^d.'i sousr,ed uai.nl;r

tho distrj-bution of S.

da:rnosun oouplex

in

^Ylest

Africa lvith partioul-ar

reference

to locations

where a13r.rer:'r'Jy ru::ulxocl

larval

populations coulc1 be forii:d iritlr-i-n and'

out-

sid,e

the

^OCP area'

Prior to iry ar::ival Drs

Coker and. [otrrson ^l:zid. oollected.

]arge

populations

of $.

-{ggggFw

larrae

from

the frhite Vo}ta

crt A-i;i-ropo}m neer

the

Akosonbo da.rr and

at

^{Botj- tr'aJ-ls ncar}

I(oforid,ua.

Ilanrae ,r"re:e obtajsed

for cytological irlentifioation

^{from each}

of the

populatlons.

In

Sogo an

er;ensive

exard-nation

of all

^{'che nrajor}

rivers

^{and sone minor ones}

1vas

made. Dry

season cond.j-tions ^',-/ere ap,'rarent i-n

al.l ^the river

^systems

i^ncluding su6h

large rivers

^{as the i,iono,}

ilo, Ihra

and

ffawa. At

many

locatlons

t'"/'"'

z5/UarT5

ze/u,ns zg/tt/ts

It

7o/1r/75

il

It

{v/rn

?/W5

r

ilWr

+/tzJtt

th{ts

25/LV75

ze/u"tll

zg/Mr

zg/W'lD 30/w75

u) laurts

R.

^I',[ono

at ^tetetou

near Tolcora:: ad.ults oaught

bitixg,

aclults reared

fron

^pupae

Dayes Elavar5ro

riJ.lago

neqr

Paline:

ad.u-Lts caught

bitjilg

R. Amoutchou

at

faf:ne73ad.ou Rc1: adr.lJ-ts :reared. from trxrpae

R.

^Ilarora

at

Gha.::a,/Iogo bord.er near

Badou: adrrlts

car"rght

bitiJ\g R;

Ilono a-b Kpessi nc-"th

of An-ie:

adi,lJ.ts caught

bitjrg

7-

eaoh

river

^v/as reduced

to long stretohos of ^pools

sone

of

^{vrhich vrere}

Ilnked

by

fast

^flounlng water

or violent cuments.

Thus

isolated.,

^goorl breedirg

sites

were

forlrd j:r lost of tl:e cososr largo qr.urrtities of Si{rjLiun

Larrrae, pupao _and.

adults

tvere

collected..

Speoial

attontion

$ras paid.

to S.

cl.arungsr4p

complex.

Where

possible adults

car.ght

bit ^g

nan

jlr _a

_{given breed,ing}

_site

were oollected.

i:r

ad.dition

to

those reared

later

^{from pupae}

fron the

sane

breed.i4g

sites. [hese

specimens have been presenred.

)n 7q" alcohol for

taxonom{c

stud.ies.

^.A, srlmra:y

of colleotion

d.ata

for [ogo is

^{shorvn belovr,}

COITNC,IIOI{ }A'IA FOR IOGO

a)

^T,anrae

nate trocation PH va.lues

6.8 6.8 6.8 5.8 6.8 6.8 6rB 6.8 6rB 8.0 7.5 7.O 715 7rO

7.5 7.5

R.

An-ie a.t

Anie -

Atapirane/Solcod.e road

R.

l.iono

at

^Tetetou

Kpinc

faIls

^{near Pal.i-ue}

Dryos Elvanyo near PalLmo Apeyome viJ.lage near SaLi:nre

R. ^Liono

at

Atch:i.::edji

R. Amoutchou

-

Pa1i.:crelbad.ou road

R.

Ilavua near ^Badou

-

GbaJao/fogo borrder Str"ean

in

^Cocoa

plantation at

Sadou

Llono

at

I{pessi iliono

at

^Tchanrba

}{o

at

Sokod.e,lBassari road

I{arr:,e near Bassari

lio at

^{Bagarr near Bassari}

i(ara

at old

lium-Kara bz:ldge

Kpelou by Sara Kavra near

lanadfura

,

Rr R"

R.

R.

D!La

DlLa

B.

..r./....

t/tiltn

st7.pfi5

w/tilt>

R. iio at

Sagan near

Bassari:

aclults reared from ^pupae

R.

I(pelou by

Snra;

^{kar'-,ta ner,r}

la.r:a-Kara: adults

reared from ^pupae Boti- fr:,I1s near i(oforid.i;a,

Ghana: adults

reared from ^pupae.

d) EXMRN,,E}1IATIOI,T

III

^{THE SOBO .A.TEA. ITPPER VOITA}

Thts

exereise vras

started

^j-Lr

the first

l,eelr

of Jarlraryr Lyl6,

^The

e:eerj.:aent vl,as oonducted. by

Dr. le Bere

^{a:.rd. l'ras rlesigned}

to internrpt control jn _the

_hope

of facilitetirrg

^-bire development

of

1a:rra1 and. pupal

stagesr

Ihe study area inolud.ed Sa:mnd.en-i, Ianvj.erae Guena, Banzo, Eangoulague,

Kulirlyion,

Lloussoud.ougou and Kar:fj-gue1a"

I

was responsible

for the

e:crnifiation

of

larva1 po2dLations, and i-ras

to

^r.urlce rccords

of

such

aspeots '- _the

^rrtjrne

of

^appearanoe

of first

finaJ-

instar la:rrae, firsi

;)upae

e-bc.ir It

v.as hoped.

that larvae

vrorrld

bo collected.

for cytolo5ical analysis

ancl

that

f'rom

the

associated ^pupae aclults

lvor,t.].d. be obtai::od.

for

taxonoaric stud.ies"

In pre-contrrcl

^(OCp) years

the

stucly area

' :s

^ioro',-rn

to

harbour S. _€glJgg9$1E

(titre) _r g.

.agm:3"os4g

(mite)

anti _1|.. q;ql_b-ai1]rq

(SirUa). It

vras therefo:re

of interest io fi::d. out

whether

all or

ai1,y

of

^-bhese species

still

prevaiJ-ed

in

i;he area

- cfter control

^l-rad. talcen ^p1ace.

Ln

.f

")

Parblcular j:rterest

t,';rs ^-i,aken i-n Guena,

a

focus

of g. Sgggry.

^Iilnere

pre-contlrol

years

this

r:pecies vras ir,rFfis3"f,sd

j:r ttffpical _forestrr

transntssj.on onchoceroiasis.

FIJLD .iORI( imIH DR,--qIup,ga-raf-w.*q!sgg$A\g)

[]:-ls

^vrorlc

tooli

approxi.::rately

the flrst

t'so t'reelcs

of i'iaroh. [he

^purpose

;f the

exercise rras

to

^lvork

i:r

assocj-ation vrith

Dr.

Gruner'raJ-d

in

as ^marsr

breed.i::g

sites

as

possible in

^areas

tlr"t

^had. becnselcc-i;ci.

,cr

^{1r.is stud.y-}

of

-l:ryter

ohAistry. I

^vras

to collect

and

id.entj{y cytologicalJy larra-I

sa"nples fxom the nane breeding

si'bes. It

l'ras hor:ed

that

these appr3achcs cou.Id

lead to

the

associ-ation

of a given r.-' ies lrith

d.efjLable lqyd.ro-cher:rica1 cond.itions and tlrtrs

facjJ.i-t^''- the reari:rg of

t,:.e

[.

-*oqlrg.sl4g spocies group under

oontrolled

conditions.

the

mai:r al-n ^y,ias

therefore to

^worlc

in

1oca.''.ities lvhere

olear Ia:rral

populations

of tho

spocies group seem

to

occLtrc

In

^Ghana

the jrrvestigations

_v/erc

oarried out

^arowrcl. Ta.r::ale,

at

Dabcrya and

Nasia on

the

Tlh:lte

Yo1tal a

^ne*1y found. i'rater

faIL

north-vrest

of

[ama-Le and

.r.r/rr..

5:

the

^Nlrrunah

falLs at

Kjrita.mpo. I.rarrae were

oollected

from eaoh

sitd.

^the

Sarekese ^d.a.il near Kuinasi,

Soti falls

^near I(oforidrta alrd

ihe

^Wh-ite

Yolta at

Ati.npolnr near Akosour.bo vrere e)calnrinecl,

In Ivory

Ooei,st,

after

discussions vrith

Dr.

Qu1i-1-lo11er€

of

OCCGE/ORSICI{ Bor-urke, sir"nueur,v,rork r-ras clone on the Maraoue

river

near Darangoro,

the

^Goue

river at

^{Oua near Dr:,nane;}

^the

^Gbe

river

^{near Zoba and.}

the

OavaIly

river

^{s,f leuP1eu.}

IvIAIERIALS _..qN!- I',IEI}{CD q

The specj-roens usecl orue from

several

sources and. a:re Srouped

irtto

the categorie

s

^{sho!-'rn beloirr:}

1) larnal

^{sa.uples froro}

^Control

^zone

II

^{and some} speoj"uens

anising

from

the

elqerj-raent conducted by

Dr. le Berre

arou::d

the

lSobo &re&r

2) ^Ianra1

^{samplcs from Togo}

^{j:r Control}

^Zone

III.

il

^{tra:sraI scurp).cs obtaj.ned}

i:i associetion

vath

Dr.

Gnrtrerryald and. those oontributed. by

Drs.

Coker and Tot'ntsortr

4)

I'Tevr\y ernerged

adults E,

-@gg,lrg ^complex speoj-mens used

i:r the

searoh

for

^po\ytene ehromosoll.es a"nd- C bancls.

3i-xed.

lanrae

^r;.rere sr"rbjected,

to

stand.artl procedures

to obta!: gient

olrromo- sone preparations as describerl.

in carlier ^repoz'ts.

lialphigkr:Lan

tubes,

^ovarian

cells

and

ttfoot

pad"str froin ed.ults vere prooessed

for

^polytene chrolrosones ^as desoribed by Yltr-ite, CoLLuzz:- nnrl Sahar

ltll>).

ffho1e aduf.ts r'rere

torn

open and

subjected. ^-bo tlr.e saroo ireah,rent v,r]:-iIe

others

lrere i)rocessecl

for

^{C Bandfug as}

reported by Boclo

(tglil o larval

^polytene chromosomes, adrrlt ilalpl::i.gh:ianr tubulesl s\rarian eel1s and

tlfoot

_{paclr! were}

atso

subjectecl

to the

C band.i.:r.,9 procedure.

NESUIflS.

In Control

Zone

I

arowrd Bobo v,rhere

treatneat

!-ras experi-nontal\y i:nter:rrpted., soue ln&ivLd.uaLs _{of -flu} -s-trb-anuq,vrere record:C. from KarfigUeLa on

the

^Conoe

river

-r.rrd. trarrviera on

the

^B1aol<

Volta.

ltro

$. _-@gg4g

^conplex

^larrae

^{were foi;nci}

at

bl:e renai.nrir6*

sites

wj.thi-n the study area nanreJy Saloand.erri., Guena, Bcnzo2 Be*:.gou1ague,

Kulfuyion

and' l,toussoudougou..

In^ontrol

^Zone

II S.

da.rrnosuxr rr,,as r:corclod

frm forr ^{sitos whlle S.}

^sirb:u:ura

r:as fotmd.

i:r

eleven

sites as

sur,unarised

il

^Table

I.

In Control

Zone

III jrl [ogo, S. S[q#p;g4, $.

S-o]rblgqse,r

.q.

qgggg.sun and

S.

sirbarrum l-rore obsellredo

Results are

sullillarisecl

il fable I'

In

^so:-ne

sites

vrithi-ir

the

study area

of Dr.

G:tttewnJ-d. and

of

Drs Co]<er ^aJId TolE:sony

apparent\r

uu:rai-xed 1.r.rva1 popr.*atj-ol1s 1?ere

found. Irt

^c'"

er sites

qpapatry vras

obsenred. Ihe re$Llts are

sgmlrarised.

ip Iable IIr

...nl ,,.'

C bandir:g

of

ocnticmere regions shovri-ng heaqy strrirr-ing lvas obserred

in larral

^polytene chromosomes

of the

S.

_@gggg

^species

gro[po [he

e:p:r'ession

of the

^C bsnd.s vras strung

in all the

speeles exoept _.$,. Sjq.1r"3jgo,gg

in

whioh

it

was

stronger and.

S. yelrPry in

wlr-loh

it

^vras strongest,

No polytene chromosomes were obserred

ln

arly

of the

ad.rrlt organs investi"gated.

flhe lr[alphtghian

firbules edrlbit

^{some vag!.le polyterry} w]r:Loir does

not ^permlt

r€so-

lution

and j-s

therefore vlrtualJy

unworlcablo.

UASIiE

I

-lEs'iinlspRcr,/I_cg,LrllgL__?_qEELr*rj[,.JIl

$anp1e SJ.ze

Speoies Proporbions

BL

So

-..N1

.*,Si

t6/t/te 26/rfi6

zo/ahS

Lo/u{'l5 tofirfiD

z5/2fi6 4/4fi5 4/+n5 Lo/rn6

u/*ta

zo/zfie z/+fia

*/pJte Lehn6 B/L{t5

8/wt5

25hn6 e4ons zz/zfie e/tofi5

1h*t5

10/L1alt5

z>/tsfi5 2v5n6

3o/3n6

5ohn6

i

C01[[R0I 201'IE

I

3Iaoir

Yolta at ^Lanviera,

^Upper

Yolta

Uppor Conoe

at Karfiguela, fl

^rr

o01{TP.0I Zo}iE

II

Wlr-lte

Yolta at

I'tragbangare Upper Vo1ta Wlr-tte

Yolta at Ya]ca.la il

^rt

Red. Yo1ta

at ZLou-Z.'abre

^rl

Tilrti;e

Volta at

itrss{s, ^Gtrana

rt

il Il ll Wavra, ^ll

illl

ll Dipare,

^It Waterfel-l

$'tr[. of

[airaler YJhlte

Yolta at

^Dipa:ee,

l, ll lt _DabOya

il

15 2

tr

:

I

BO

1o 26 16 55 55 22 9

w IL

28 L4

w

?a

l.B LL 13 5

14

N

15 10

u

9

o"9tT

:

80 30 25 r.6

5'

55 22

ra w }L

18

IL

10 4

:

7 10 9 l-0 L2

7O

?J

:

3

LO

L8

I

L

rl il

_Daboya,

R. So:ri at

tranliatare Rr Dalca near Ila.nd:ipe

R.

Daka

at

^PoLnt

II

Ghana ll It

tl il

ft tt

. colIIROt

ZOIiE

IIl

R. Otl

near Sanssanne }liango, Togo

R.

Satique near

Dapa^n6o

^rf

R.

Kara

at la.rna-I(a::a

^tr

06nflLuenoe

of

Kara and.

KueIL

^it

R.

^I/lo

at

^Bagan near

Bassari

^rl

R.

^i,lono

at KBesei

^rr

n.

^IUono

at fetetor

^rr

R.

Iiiono

at [etetou

^r?

R. Degou

^MaI[

Ro Bor.fle near Borura6lq vi 11age

I

^UaJ.i

Legemd:

Bi

₌

g. sqgaroqEg

So =

!,.

ggfpg.B.$tr

Si = S.

sirbantm

-

^{$1 =}

E.

_-4gggglgl

??*

= Identity

uncerbain

7

TASIE

IIX

RESIIL$S I'ROI.{ STUDY AIEIIS OF DRS. ^COICER AI'{D TOIIN'TSON GRIIi$E,rVAI'D

")

b)

DATE

w/tt tts t7/tutr

70/5n6 30/3fi6

*/ilta

zpJz/ra

27hn6 zuzJta

25/2rt6

q/*ta

zz/zlte 7/3n6

7hn6 7hn6

a/nfi6

IOCAIIONS

Volta R. at

AtJ.npoiar, Akosombo,

Botl falls

^near l(oforldua

r)

Ro Degor

R.

3ou1e near Sonnabio

village

Samtrrle

Size ^a

Specj.es Proportions

Bl Ya So Nl.SL

Girara

il

hIa].i

il

1.65 B6

74

%z

29 L5 20

LLo 5'

LL0

9

t7 ,10

1e

IL

L6

5- :

fr It

I

10

t5

i-B

IL

16 14 9 29 18 24 70

I

Rr l,tono

at

Ietetou- fogo 2 R1 Kara

at

la.na-Kara Brid.ge,

Rr Oti at

^{Sausaru:e l,.{or4;o}

l'Ihite Volta at

Daboya

White

Volta at

^i{asia

Nkruah

fe'I].s

l(lrrtanpo

^rt

Ifa.ter fa'I,'l

ld.ffr of faina-l.e

^r!

Rr

CavaJJ;r

at leupleu lvory

Coast Ro Gbe nea.y

Zoba ll

^tl

R.

Goue

at Ona tl

ll

I R.

^I'larague ncar Darangoror ^It

Iogo

ll Ghaxa

il

a I

1

t

:

4

tregend.: BL ₌

E.

SSyEgoEg

Ni = S.

d.a:urosrxo

*

_Some

of

the

Ya ₌

9. r,ahe4*

^So ₌

_E,

Sg&rgnse

Si = S. sjrbsruan

^??

₌ iflsnlity

unoertaia data

in thj.s

IabLe

is

ert::acted. from Table

I.

0BryRYA!-L0I,l-Sl/.c00,!rr{ts

The

distribution of the

species,

E. _@ggg

^and.

$.

s+cbe]rrp, reporbect

earlier in Control

Zoae

II

^has expand.ed as r,raterja.l from more

l.ocal.itles

has been anal;rsed"r

$Iith the

exception

of a silgle 1ocal-iff, the

nevrly found. vrater:falI t{rYIr

6f

[ama-].s where

E. €geg9gl$

^seems

to

^{breed., n'I.'I}

sif,ss

i-nvestigated featr.ued no speoies

other

tkrarl

g.

d.aruqo_sJm, and S. ,sjr.ba"qIg, ^Tire

pattern of distribution of

theso two specj-es does

not

seem

to

have

changod. fhe identlty of the

species

e:<aiui.::ed. fronr -bhewa'';c':la-U-1{,}Tr

of

^faraa'l.s has

not

becn asoerbained. because the ruateria-l-

frou the

area ir'rds rrnsr.eitable

for

^good. chro.losoraal prepar:ationso

i. ../ . ...

4B

Results fron the

^p11ot exerpise

in

^Contro]- Zone

III are

incl-ud.ed

to give a bri-ef picttue of the

species somposition

i:r the arear

ConseErentJy ouJy

a folr

speoiraens from lcey

sites

l.rere ana\rsed.;

0omrents on speoies

cListribution

and- rarr.ge r'riJ-J. be f,ror.e approprj.ate when

larger

samples ooverc.ng

vaster

areas

arc

ana\rsed.,

[he 0

band.lIg

of

centronere regions obsezved. i.:e

la::nal

poJ;ytene

ohronosomes mere\r emphasis _sirn'i'l s3 obsenratioas using conventional sta:ini::g

tecluulques'

Ur.ljJre

the situation

,,vhere 3ec1o

(tglil

reporbed.

a reversal of

ths rfo _ba,nding pattez:xrt fu.Q..

ggklg vrith

respect

to that

^j-n

s.

-o.ega-Llrr.eisl

the

c banclfug

of

centronrerc

regions in tha S,

da,anosu.n species group

is

nstandarri.n

E.

-Egg3g.qryn and

S'

_vqheqss sirov,r

very

hear6r ste.ini:r"g

of the

re8ton

but

no reyersa-ls

are

j-nvoIved. r,vith respect

to

standarrd.

W-qu4tsrEB

: ^{Further analysis of}

rnaterj-a,l from

control

zones

rr

and

rfr.

Further

sea:roh

for

^po]rytene chronosomes i.:: adultso

T Collaboration

rulth Sector

chiefs

as amanged W

Drs.

tre Be::re and lyalslr.

BNFENE}TCES

1.

^Bed.o, D'G.

19?5: c

Banding

rn

Po\rtene ohromosomes

of

s.irnnl}rm ornatipes arr.d

S.

nelatrm

Chromosooa

(eerl,)

_DL,.2g]-

-

^3@

2.

^{Wlt-tte, GrSo}

_r

IJ

Coluzzir A.R. Zat:ar L9l5t

^Rerrj.ew

of

Cytogenetic studies on AnopheU:re Yectors

of

I{Iala^r:ia.

WHO Mfurcographed. Dooument:

\fiIo/7trM,n5,849,

Y:[IIO/\rBCft5.55B'