الدراسة الفيتوكيميائية و دراسة النشاطية المضادة للأكسدة والبكتيريا لنباتات العائلة الخيمية المستعملة في الأكل بمنطقة وادي سوف

عوضوملا

-دادعإ نم

:

.ةجيدخ يناجت و فافع هقزر

22

/

60

/

2602

أ مسق رضاحم ذاتسأ ةقيفش الله قزر .د

"اسيئر"

يداولا ةعماج

أ مسق رضاحم ذاتسأ ةفيلخلا دمحا هسمش .د

"ارطؤم"

يداولا ةعماج

ديمحلا دبع بيبحل نب .أ

أ مسق دعاسم ذاتسأ

"انحتمم"

يداولا ةعماج

: يعماجلا مسوملا

8102

/

8102

ميحرلا نحمرلا للها مسب

''

نورفكت لاو لي اوركشاو مكركذأ ينوركذاف

''

دملحا

.لمعلا اذه ماتملإ انقفو يذلا لىاعتو كرابت لىوملل ركشلاو

انيلع لخبي لم يذلا ةفيللخا دحمأ ةسشم روتكدلا لضافلا ذاتسلأا لىإ ليزلجا ركشلاب مدقتن كلذك

هجارخإ و لمعلا اذه ماتملإ ةمئادلا هتعباتم و هردص ةعس و هبرص ىلع و ،هتاهجوت و هتاداشرإب

جرن و ةوجرلما ةروصلاب

انع للها هازجف ،ةيفاعلا و ةحصلا ماودب هيلع نيم نأ ىلع و ىلج للها نم و

.ملعتلا و ملعلا ةبلط لكل ارخف و ارخذ هلعج و ءازلجا يرخ

اذله ةنجللا ةسائر الهوبق ىلع ةقيفش للها قزر ةذاتسلأل نانتملاا ليزج و نافرعلا بيطأب مدقتن و

لمعلا

ماترحلاا و ريدقتلا قئافب مدقتن امك

ةنلج في ةكراشلما هلوبق ىلع ديملحا دبع بيبلح نب ذاتسلأل

.اذه انلمع ينستح ةدايز في انديفي يذلا ميقلا هيجوتلاب انثبح ءارثإ و ةشقانلما

مراكلأا انتذتاسأ لك لىإ ريدقتلا و ركشلا تارابع غلبا و ىسمأب هجوتلا انب رديج يرخلأا في و

يط اننيوكت في اوهماس نيذلا

بيرق نم اندعاس نم لك و انتعفد ةبلط لك و ،يعمالجا انراسم ةل

.ءاهتنلاا ةياغ لىإ ةيادبلا نم لمعلا اذه زانجا في ديعب وأ

ءادــــهلإا

احبس لاق نيذلا لىإ اذه يلمع يدهأ

ن

:امهيف لىاعت و ه

رلا نم لذلا حانج امله ضفخاو (

اضيأ لاقو ... ) ةحم

يبر لقو

حمرا

ه

ام

ايرغص ينايبر امك

نم لىإ )

لبذت لا تيلا يتايح ةرهز لىإ... تطعأو اهسفن تمرح نم ... تبرصو تدهاج

....

. ةبيبلحا يمأ ... ىنفي لا يذلا نانلحا عبن

لىإو

ملعلا تاجرد ىلعأ يقترأ نأ لجا نم ةايلحا في اسورد نينقلو نيملع يذلا يبأ

اهمازجو اهمرمع في لاطأو للها امهظفح

. ءازلجا يرخ انع للها

قئاقش... ناتسبلا راهزأ... ةايلحا عوشم ... ىسنأ نأ نينكيم لاو

نامعنلا

.

ةيرغصلا و ةيربكلا تيلئاعو ... يتاوخأو يتوخإ...بردلا دناسم...

ةصاخو

...ةلئاعلا معارب

.

يلمع يدهأو

..

بردلا اذه ينوكراش نم لىإ

يحاجنب اوجهتباو ..

يجرختب اودعسو

و ةكوبرم و ةمطاف..حانج يتاقيدص

لىإ

و يئلامز

مز

ي

لا

يت

يفكت لا نيذلا

ةرابع مه

نانتملاا و ماترحلاا

و

يعمالجا لىإ يئادتبلاا روطلا نم يتذتاسأ ةفاك لىإ

مهركذلأ ةركاذلا نيفعست لمو ينوفرـعو مهتفرع نم لك لىإ

....

مسق ةبلط لكلو

لا

ايجولويب

ةعماج في

.... رضلخ هحم ديهشلا

بيلق مهعسو نم لكلو

... اذه يلمع يدهأ تيحفص مهعست لمو

صخللما

Abstract

Résumé

3 Apium graveolens Petroselinum crispum Coriandrum sativum P. crispum A. graveolens 0.206) ± 30.67 ± 0.184 02.00 C. sativum ± 0.146

88.00

• DPPH A. graveolens C. sativum =275.65±5.06) 50 IC =288.28±3.62 50 IC P. crispum 25.11 ± 302.12 = 50 IC =62.24±1.64 50 IC C. Sativum

P. crispum . A. graveolens Coriandrum sativum Petroselinum crispum Apium graveolens DPPH

Abstract

The aim of this work is to determine the total phenolic content and the antioxidant and antibacterial activity studies for the extracts of the aerial part of 3 medicinal plants in the region of Eloued, in South-East of Algeria: Apium graveolens, Petroselinum crispum, Coriandrum sativum.

In General, The results showed That total phenolic contents of aerial parts have changed from one plant type to another P. Crispum and A. gravelolens with polyphenols where the quantitative content of these substances was estimated at (78.03±0.206) and (69.88±0.184) mg is equivalent to gallic acid per gram of the extract, as for C. sativum The amount of phenolic content has a value (55.81±0.146) mg equivalent of gallic acid per gram of extract.

While the antioxidant activity tests by using the DPPH• method show the methanol extracts of the plants studied A. graveolens and C. sativum have antioxidant effect with an inhibitory capacity of (IC50=275.65±5.06),

(IC50=288.28±3.62) and P. crispum was less active valued (IC50=302.12±25.11)

compared to ascorbic acid (IC50=62.24±1.64).

The tablet diffusion method was used in the study of anti-bacterial activity, where the results showed that the methanolic extract of C. sativum is one of the most effective extracts against Gram positive and Gram negative compared to extracts of P. crispum and A. graveolens.

Key Words:

Coriandrum sativum, Petroselinum crispum, Apium graveolens, Poly phenols, Antioxidant activity, DPPH• test, Antimicrobial activity.

Résumé

Le but de ce travail est de déterminer la teneur totale en phénol et études l' activité antioxydante et antibactérienne, des extraits de la partie aérienne de trois plantes médicinales de la région d' Eloued, au Sud-est de l' Algérie: Apium graveolens, Petroselinum crispum, Coriandrum sativum.

En général, Les résultats on montré que le contenu phénolique total des parties aériennes des plantes étudiées varie d'un type de plante à l'autre P. crispum et A. graveolens en des poly phénols, le contenue quantitatif de ces substances étant estimé à (78.03±0.206) et (69.88±0.184) mg d'équivalent à l'acide gallique par gramme d'extrait, comme pour la C. sativum. La teneur en contenu phénolique a une valeur (55.81±0.146) mg équivalent à l'acide gallique par gramme d'extrait. DPPH •

Alors que les tests d' activité antioxydante utilisant par la méthode DPPH• montrent que les extraits au méthanol des plantes étudiées, A. graveolens et C. sativum, ont un effet antioxydant et un pouvoir inhibiteur de (IC50=275.65±5.06), (IC50=288.28±3.62) et P. crispum était moins actif

(IC50=302.12±25.11) comparé à l'acide ascorbique (IC50=62.24±1.64).

La méthode de diffusion sur comprimés a été utilisée dans l' étude de l'activité antibactérienne. Les résultats ont montré que l' extrait méthanolique de C. sativum est l'un des extraits les plus efficaces contre les Gram positifs et Gram négatifs par rapport aux extraits de P. crispum et de A. graveolens.

Les Mots clés:

Coriandrum sativum, Petroselinum crispum, Apium graveolens, Poly phenols, Activité antioxidant, Test DPPH•, Activité antimicrobienne.

L Apium graveolens 1 Apiaceae (Umbelliferae)

0

0

0

2

2

Apium graveolens L

2

0

2

2

A. graveolens

2

7

Apium

2

4

A. graveolens L

2

8

Apium graveolens L

2

0

A. graveolens L

2

0

A. graveolens. L

2

2

A. graveolens

2

06

A. graveolens L Linn Coriandrum sativum

0

Coriandrum sativum Linn

0

0

0

2

0

7

Coriandrum

0

4

C. sativum

0

8

C. sativum

0

0

Coriandrum sativum

0

3

C. sativum

0

0

Coriandrum sativum L

0

2

C. sativum

0

06

C. sativum Linn crispum Petroselinum

0

Petroselinum crispum

0

0

0

2

0

7

Petroselinum

0

4

Petroselinum crispum

0

8

Petroselinum crispum

0

0

Petroselinum crispum

0

3

Petroselinum crispum

0

0

Petroselinum crispum

0

2

Petroselinum crispum

0

06

Petroselinum crispum

0

0

0

0

2

0

7

0

4

0

8

0

0

2

2

0

2

2

7

4

4

0

DPPH

4

2

50 IC DPPH

8

8

0

8

7

8

4

8

8

8

0

0

0

0

R

0

2

0

7

0

4

2

2

0

2

2

2

7

2

4

63

60

A . graveolens L

62

A. graveolens. L

67

A. graveolens L

64

Coriandrum sativum

68

C. sativum

60

Petroselinum crispum

63

crispum P

60

62

C. sativum A. graveolens P. crispum

60

Macération 38

62

47

67

Acide Ascorbique 48

60

A. graveolens. L 10

62

066

12

67

C. sativum 18

64

19

68

20

60

Petroselinum crispum 26

63

066

27

60

33

62

34

06

35

00

36

02

46

07

mg EAG/g EP 47

04

49

08

6 5 IC

86

 DPPH 50

00

( C. sativum A. graveolens P. crispum 51

قـــ

صتخلما ةمئا

ـــــ

تار

AA% Absorbation Activité I % IC50: Inhibition Concentration 50%. mm Millimètre L Litre µg : Microgramme Mg Milligramme g gramme

W8O23: Oxyde Tungstène. % : Pourcentage.

Vit C : Vitamine C (Acide Ascorbique) R %: Pourcentage de rendement. ROS : Resctive Oxygen Species. Na2CO3: Carbonate de Sodium.

Mg EAG/g MP: Milligramme Equivalent Acide Gallique sur Gramme

des Matières d'Extraits.

PPT Polyphénoles Totaux. Pa Pseudomonas aeruginosa. Escherichia coli. Ec Staphylococcus aures. St

2009 2007 2011 Ali-shtayeb, 2008) Majed et

  • DPPH   Apium graveolens L

,

Petroselinum crispum

,

Coriandrum sativum

.



ءزلجا

رظنلا

لولأا لصفلا

تابن

1 Apiaceae Umbelliferae

1

1

446 3540 2013 al et seraga 2013 chibani 2010

1

2

   ombelle Involucre    Mericapes 2007 2008 2013 chibani,

2

Apium graveolens L

2

1

1323 2013

466

266

800 1699 1806

2600

2

2

A. graveolens A. graveolens 2014 al Helaly et 2015 al (kooti et (Kamalesh, 2008)

11

A . graveolens L. ( 2600 , .(mapa-de-africa

2

3

Apium 20) (25 Apium Ronse et al.;2010) . graveolens L. A A. nodiflorum (L.) Lag. repens (Jacq.) Lag. A.

A. crassipes Rchb.f. A. bermejoi L.

2

4

A. graveolens L

Céléri

Celery

Apium

graveolens

L. Marsh parsle Achedes marais Acedouce Smallage

،يباقعلا(

8112

.)

Umbelliferae (Apiaceae)

2603

12

A. graveolens. L

2

5

Apium graveolens L.

06

26

Kamlesh R et al.,2008     Rożek Renata Nurzyńska et al., 2016

7.5-2 5- 2

Sutrisna et al., 2015

 Umbel

 Schizocarp 5 2-1 (Seraga et al, 2013

13

A. graveolens L. Hussain et al., 2013

2

6

A. graveolens L.

11

A. graveolens. L

plants.usda.; 2018

Plantae Règne Tracheobionta Sous-règne Spermatophyta Super- Embranchement Magnoliophyta Embranchement Magnoliopsida (Eudicots) Classe

Apiales Ordre Apiaceae (Umbelliferae Famille Apium Genre Apium graveolens L. Espèce

2

7

A. graveolens. L Limonene Selinene Flavonoids Frocoumarin glycosides C B A (Garg et al., 1980)  Norcarotenoid glycosides

2

Coumarins Celereoin Flavonoids Apigenin) Limonene

.(Kamalesh, 2008; Rozek, Renata Nurzynska et al.,2016) 

20

Terpenes Sesquiterpens

0.8

%3 Limonene %

06

s-Selinene

22.27

%

( Rozek, Renata Nurzynska et al.,2016)

2

8

A. graveolens. L

12

066

2600

,

(USDA

111

00

0.74

0.0

6.03

6.02

A

22

B1

6.620

2

B

6.683

B3

6.726

5

B

6.240

B6

6.634

C

7.0

E

6.23

K

22.7

46

6.26

2

9

A. graveolens

00

24

206

06

6.07

28.47

1990 kapoor

0208

(Teng CM et al., Prof. Satyanand Tyag et al., 2013

2

11

A. graveolens L.  Din Ud Zakir

2608

 Furanocoumarins monocytogenes listeria Escherichia coli 2005 al et Sipailiene  Wesam Kooti

2608

(mg /g)

80.62

 Din Ud Zakir

2608

• DPPH 50 IC µg/ml

0.40

يناثلا لصفلا

تابن

ةربزكلا تابن ةسارد

Coriandrum sativum Linn

1

1

Apiaceae 2011 al et veram 2006 1550 2011)

،

Anuradha and Deepa

.)

1

2

1988 1984 al et Simou

1

3

Coriandrum 2012 al et Dharmalingam

عاونلأا نيب نم و

اراشتنا رثكلأا

Coriandrum (Diederichsen.A ;1996)

1

4

C. sativum

:يسنرفلا مسلاا

Coriandre

،

:يزيلجنلاا مسلاا

Coriander

مسلاا ،

يملعلا

:

Coriandrum sativum L (Diederichsen.A ;1996) 2010

-(Diederichsen.A ;1996)

14

Coriandrum sativum

1

5

C. sativum 30 70 (Diederichsen.A ;1996)

15

C. sativum R. Kilian , Schultze-Motel 1986)

1

6

Coriandrum sativum

13

(USDA.,2013) C. sativum

Plantae Règne Tracheobionta Sous-règne Spermatophyta Super- Embranchement Magnoliophyta Embranchement Magnoliopsida (Eudicots) Classe Rosidae Sous-Classe Apiales Ordre Apiaceae (Umbelliferae Famille Coriandrum Genre Coriandrum sativum Espèce

1

7

C. sativum 0.03 2.6 % 2013 al et Nadeem linalool 65 70 (% citron borneol pinene limonene 2005 Wallis and epoxideviolaxanthin cryptoxanthin β epoxide 6 5 (Lutein carotene β neoxanthin carotene β 61.14 % 2003 al et Almeida De

1

8

Coriandrum sativum L

066

1999 Bakhru

14

6.3 % 0.6 % 83 % 3.67 % 1.2 % 3.3 %

-C

066

15

6.3 % 12.1 % 2 11 % 6 21 % 3 30 % 1 14 % 4 4 %

1

9

C. sativum 2004 al et Behera 2004 al et Cantore LO Eidi et al.,2012)

1

11

C. sativum Linn)  Ono

0220

C. sativum Escherichia coli Staphylococcus aureus  1998 Fleming C. sativum Escherichia coli  2005 . al et Chericoni C. sativum Linn)  2002

ثلاثلا لصفلا

تابن

1

Petroselinum crispum

1

1

2666

0220

1

2

P . crispum

2663

1

3

سنجلا ةسارد

Petroselinum Apiaceae Petroselinum (plants.usda., 2018) P. crispum (parsley). P. hortense Hoffm. P. sativum Hoffm. - P. vulgare Lagasca.

1

4

Petroselinum crispum Persil Parsley Petroselinum crispum Quezel, P; Santa, S 1963

-Apiaceae .(Agyare.C et al.,2017)

16

Petroselinum crispum Svetlana et Dorina., 2012)

1

5

Petroselinum crispum

28

06

2668

   

2

7

 

2663

17

P. crispum

2663

1

6

يتابنلا فينصتلا

Petroselinum crispum

16

Petroselinum crispum

Quezel et Santa, 1963)

)

Plantae Règne Tracheobionta Sous-règne Spermatophyta Super- Embranchement Magnoliophyta Embranchement Magnoliopsida(Eudicots) Classe Rosidae Sous-Classe Apiales Ordre Apiaceae (Umbelliferae) Famille Petroselinum Genre Petroselinum crispum Espèce

1

7

Petroselinum crispum Myricetin (El Sayed et al,2018) Apigenin

(Chaves Dsa et al,2011) glycosides

Acide ascorbique Vitamine C" (Abou El Leel of et al,2017)

Huiles essentielles Myristicin 12.6 % Apiole %

07.2

(24.2%)1,3,8-p- menthatriene β-phellandrene (22.8%) terpinolene (10.3%) ;2016 et al M Snoussi

1

8

Petroselinum crispum

17

066

USDA.,2018)

111

70

0.7

6.8

7

C

077

E

6.3

K

0046

B6

6.0

070

0.2

86

80

884

80

0.0

6.0

6.2

6.0

03.3

1

9

Petroselinum crispum .(El-Sayed et al,2018)

-Mert A et al,2017)

-( Stan.M et al,2011 ; Pápay Z.E et al, 2012)

.(Linde G.A et al, 2016)

-Apigenin (Pourmorad et al;2006)

1

11

Petroselinum crispum P. crispum

 University of Missouri P. crispum Apigenin USDA, 2018)  Linde G.A et al,2016)

Apiole Myricetin  (Svetlana et Dorina.,2012) P. crispum (µg/ml

78.06

 El Sayed

2600

P. crispum = 168.98±3.85 50 (IC

لولأا لصفلا

1

1

1

Apiaceae Apium graveolens Petroselinum crispum Coriandrum sativum 2018

1

2

2014, 2000 2006

(2006

1

3

60

18

Becher Entonnoier Papier filtre Papier aluminium Erlenmeyer Spatule Micropipette Eau distillé Méthanol Matériel végétale Balance analytique Rotavapeur

1

4

(09)

19

PPT Becher Tube a essais

-Micropipette Papier aluminium Support de tube a essais Spatule Le cuves Les extraits de plante Eau distillé Méthanol Folin ciocalteau (10%) Carbonate de sodium Na Co3 (27%) Acide gallique Balance analytique Spectrophotomètre

1

5

06

11

DPPH

.

Becher

Papier aluminium

Tube a essais Support de tube a essais

Spatule Le cuves Micropipette Les extraits de plante Méthanol Acide ascorbique DPPH (2,2-diphenyl-1 picrylhydrazyl) Balance analytique Spectrophotomètre

1

6

00

11

Becher

Micropipette

Pipette pasteur

Support Les extraits de plante

Ethanol Muller Hinton

نازنب دقوم

Bec de Benzene Etuve

-Autoclave

2

2

1

Macération 20g 24 24 Rotavapeur

45 C°

. (Markham KR,

1982; Bruneton J, 1999)

11

Macération

2

2

(Guettaf et al., 2016 20 g 24 h Macération Filtration Evaporation 03

3

Singleton et rossi al.,1965

Folin- Ciocalteu Folin- Ciocalteu (W8O23 MO8O3) DIF, 2015 125 µl 1mg 1ml 500µl 125µl Folin- Ciocalteu 3 1250µl Na2 Co3) 1ml 90 nm

306

.(Slinkard .K et al.1977 Acid Gallique 2 mg 2 ml 500µg/ml µg/ml 400 300 µg/ml µg/ml 200 µg/ml 100 µg/ml 50 25 µg/ml Acid Gallique

4

• DPPH -1 -2,2'Diphenyl picrylhdrazyl

4

1

• DPPH

اذه

• DPPH • DPPH

اذه

• DPPH (Dziri et al., 2012) • DPPH • DPPH 0.1mM 4mg • DPPH 100ml Méthanol mg/ml)

6.8

mg/ml

0

mg/ml

0.8

2mg/ml 2.5mg/ml

mg/ml)

6 0

mg/ml

6.2

mg/ml

6 7

0.4mg/ml 0.5mg/ml 1ml 200μl 800μl • DPPH M m 0.1 3 30 Spectrophotometer 517nm  C % I • DPPH • DPPH Ac Contrôle As • DPPH : I % .

4

2

50 IC • DPPH I %= [(Ac –As)/Ac] × 100

C 50 IC • DPPH 50% • DPPH %) (I

.(Ramesh D et al,2015 ;Aktumsek A et al,2011)

5

هذه

اذه

{03 Pseudomonas aeruginosa ATCC27853 Pa Escherichia coli ATCC25922 Ec Staphylococcus aureus ATCC6538 St

5

1

Escherichia coli Enterobacreriaceae 1 3 µm 37C

(Mellies et al .,2007; Silivia M.,2003; ;SinglentonP.,2004)

1µm

2602

2009) Adegoke A et al.,

)

Pseudomonas aeruginosa

يه

ةيئاوه

2004 Singlenton .(Hidron A et al.,2008)

5

2

Anse de platine gélose nutritive Etuve 37c°

24

2607

5

3

Muller-Hinton Autoclave 121C° 9cm MH de benzene Bec

(

2008 A Mitra and M Chakraborty

)

5

4

ml

8

2662

5

5

7

60° (Chakraborty M and Mitra A.,

2008)

5

6

4

7

2mg/ml man Wath 6mm Micropipette 20µl Cefalexine

37C°

24

mm (Chakraborty M and Mitra., 2008)

يناثلا لصفلا

ةشقانلما و جئاتنلا

1

1

R% % 2016 GETTAF et al

12

% G

21

Coriandrum sativum g

2.0

%

07

Petroselinum crispum g

7.0

%

08.8

Apium graveolens.L g

2.2

%

04.8

: : %

07

%

08.8

%

04.8

P. crispum . graveolens A sativum C

 PPT Singleton et rossi,1965) Folin –Ciocalteu Acide gallique"

62

07

12

13

mg EAG/g EP)

C. sativum P. crispum A. graveolens ± 0.146

88.00

± 0.206

30.67

± 0.184

02.00

-0.2 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 0 100 200 300 400 500 600 د نع ةي صا صت ملإ ا nm 760 زيكرتلا ( mg/ml) y= 0.002 x-0.004 R²= 0.995

30.67

C. sativum A. graveolens mg EAG/g EP) ± 0.146

88.00

± 0.184

02.00

1

3

 • DPPH • DPPH (Bouzghale B ;2008) Acide ascorbique nm

803

67

13

Acide Ascorbique

14

µg/ml

866

C. sativum

32.26

% A. graveolens

00.86

% 0 10 20 30 40 50 0 20 40 60 80 100 120 I % Concentration µg/ml AA%P. crispum C. sativum AA % AA % A. graveolens µg/ml

0.00

32.2602

6.23

00.86

866

8.24

07.43

0.22

30.2224

6.28

32.00

466

0.06

82.00

4.43

86.2338

6.03

44.02

766

0.02

70.02

7.76

74.2036

3.00

70.47

266

6.04

02.27

6.00

00.3406

4.02

26.20

066

µg/ml

466

07.43

%  50

IC

50 IC

86

% DPPH

08

50 IC

15

6 5

IC

86

%



DPPH

A. graveolens C. sativum P. crispum Vitamine C 1

IC

5 µg/ml)

238.08

8.60

200.20

7.02

762.02

28.00

02.24

0.04

1 5

IC

A. graveolens C. sativum • DPPH crispum . P 1 5 IC µg/ml

238.0838

1 5 IC

200.2033

1 IC 5 1 5 IC P. crispum µg/ml

762.020

1 IC

1

4

C .sativum P.crispum A. graveolens

00

16

C. sativum

A. graveolens

P. crispum

Bactérie C. sativum P. crispum A. graveolens Antibiotique Pseudomonas aeruginosa 14 mm mm

04

13 mm 25 mm Staphylococcus aureus mm

06

8 mm mm

2

30 mm Escherichia coli

02

mm 10 mm mm

2

mm

28

Pseudomonas aeruginosa K A. graveolens M P. crispum D C. sativum AB Anti-biotique Cefalexin)

دهاشلا

Ethanole

( ةقيثولا

19

)

ريثأت :

C. sativum A. graveolens P. crispum

.ةربتخملا ةيريتكبلا تلالاسلا ىلع

mm

04

mm

07

Cefalexin mm

28

 Staphylococcus aureus mm

06

mm

2

mm

0

CN mm

76

 Escherichia coli C. sativum P. crispum .graveolens A mm

02

10 mm mm

2

CN) mm

28

1

Ethanol

2

Duraffourd et al.,1990)

2

1

crispum P. C. sativum A. graveolens Djemai.,2009)

2608

2

2

P. crispum ± 0.20

30.67

El-H Tang

2608

mgGAE/g

0.24

±

24.33

(El Sayed et al.,2018)

±0.2) mgGAE/g

40.07

( Zakir Ud Din et al,2015; Jung et al,2011)

±0.18

02.00

et al.,2012) (Shahwar.M C .sativum mgGAE/g ±3.42

76.28

6.04

±

88.00

Hayouni et al., 2007 et al,2011 ( Albuquerque et Hanazaki, 2006; Toledo ( Ksouri R et al., 2008) Kähkönen et al., 1999 Rebiai et al., 2013;

2

3

El Sayed

2600

DPPH

±25.11)µg/ml

762 02

Hinneburg

2660

86 IC µg/ml

6.06

±

02.6

Zakir Ud Din

2608

A. graveolens  DPPH 86 IC µg/ml

6.23

±

0.40

µg/ml ± 5.06)

238.08

D.B Muniz Marquez

2604

C. sativum DPPH 86 IC µg/ml

6.27

±

07.02

86 IC 3.62) ± µg/ml

200.20

86 IC NOTO et al.,2016) DPPH P. crispum A. graveolens (C. sativum IVANA, 2011) (Javanmardi et al.,2003)

• DPPH .,2012) al (Yordil et

2

4

C.sativum A. graveolens P. Crispum (IVANA, 2011) Lambert.,2002)

2662

Transpeptidase

Peptidoglycane

ADN ADN

graveolens. L Apium Petroselinum crispum Coriandrum sativum P. crispum C. sativum graveolens. L A  Rossi and Singleton

ciocalteau-Folin P. crispum EAG/g EP mg 78.03±0.20 mg EAG/g EP A. graveolens 0.18 69.88± C. sativum mg EAG/g EP 55.81±0.14  DPPH 0 5 IC ±25.11) µg/ml

762.02

,

7.02

±

200.20

, ±5.06

238 08

50 IC ml) / µg

Pseudomonas aeruginosa; coli Escherichia Staphylococcus aureus "Pseudomonas aeruginosa" 14 mm 14 mm 13 mm

1

2662

.

2

0220

0

3

0200

286

280

4

2608

73

7

000

030

8

2663

240

0

2660

0

3

2607

08

062

0

2604

70

2

2606

Apium graveolens L.

08

2

06

2603

Apium graveolens L.

00

2663

22

02

2660

404

07

2600

04

2662

70

72

067

08

2662

2

2

02

00

2663

3

700

03

2602

lonadioides Coss Rhetinolepis

06

00

2606

066

02

2666

02

07

72

26

2660

778

736

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DPPH A. graveolens y = 0.179x + 1.659 R² = 0.922 0 20 40 60 80 100 0 100 200 300 400 500 600 ط يبث ت ة بس ن I% زيكرتلا µg/ml y = 0.1751x + 0.7439 R² = 0.9468 0 20 40 60 80 100 0 100 200 300 400 500 600 ط يبث ت ة بس ن I% زيكرتلا µg/ml y = 0.1724x + 2.3033 R² = 0.9292 0 20 40 60 80 100 0 100 200 300 400 500 600 ط يبث ت ة بس ن I% زيكرتلا µg/ml

DPPH P. crispum y = 0.1712x + 2.7897 R² = 0.9622 0 20 40 60 0 50 100 150 200 250 300 350 400 450 ط يبث ت ة بس ن I% زيكرتلا µg/ml y = 0.1152x + 11.302 R² = 0.9721 0 20 40 60 80 0 50 100 150 200 250 300 350 400 450 ط يبث ت ة بس ن I% زيكرتلا µg/ml y = 0.1534x + 4.7926 R² = 0.9991 0 10 20 30 40 50 60 70 0 50 100 150 200 250 300 350 400 450 ط يبث ت ة بس ن I% زيكرتلا µg/ml

DPPH C. sativum y = 0.1604x + 3.4764 R² = 0.9437 0 20 40 60 80 100 0 100 200 300 400 500 600 ط يبث ت ة بس ن I% زيكرتلا µg/ml y = 0.1651x + 1.8598 R² = 0.9486 0 20 40 60 80 100 0 100 200 300 400 500 600 ط يبث ت ة بس ن I% زيكرتلا µg/ml y = 0.1634x + 3.7196 R² = 0.9672 0 20 40 60 80 100 0 100 200 300 400 500 600 ط يبث ت ة بس ن I% زيكرتلا µg/ml

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