Ultrasound-assisted extraction of Ziziphus lotus’s bioactive compounds: Optimization by Fractional Factorial Design 27-3 (FFD)

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Réf : ……./UAMOB/F.SNV.ST/DEP.BIO/2018

MEMOIRE DE FIN D’ETUDES

EN VUE DE L’OBTENTION DU DIPLOME MASTER

Domaine : SNV Filière : Sciences Biologiques

Spécialité : Physiologie Cellulaire et Physiopathologie

Présenté par :

SIAD Rabie & MOUSSA Hamza

Thème

Ultrasound-assisted extraction of Ziziphus lotus’s bioactive

compounds: Optimization by Fractional Factorial Design

2 7-3

(FFD)

Soutenu le : 27

/ 06 / 2018

Devant le jury composé de :

Nom et Prénom

Grade

M. REMINI Hocine

MCB

Univ. de Bouira

Président

M. DAHMOUNE Farid

MCA

Univ. de Bouira

Promoteur

Mlle. BARKANI Farida

Doctorante

Univ. de bouira

Co-Promoteur

M. BARA Mouslim

M. HAMZAOUI Soufiane

MCA

Univ. de Bouira

Examinateur

Invité

Année Universitaire : 2017/2018

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Table

of

list

Figures

lht

Tables list

INTRODACTION

Chryter

I:

Literature

Review

(3)

3.1.1.3. Objectives of

protocol

...12

3.1.1.4. Factors and

levels.

...12

3.l.l.5.Choiceoftheexperimentaldesign..

...13

3.2,

Fractional factorial

designs and

optimization

..,...

...14

3.2.1. Preparation of the experimental

plan..

...14

3.2.1.1. Study

Description

...14

3.2.1.2. Responses, Factors and

levels

...14

3.2.I.3. Choice of the experimental

design..

...15

4. Comparison of the different techniques

for

phenolic compounds

extraction

from

Ziziphus

lotus

fruit.

4.1. Ultrasound assisted extraction

(UAE)...

...16

4.2. Conventional solvent extraction

(CSE)..

..f

...

...16

4.3. Microwave assisted extraction

(MAE)...

...17

5, Phenol

index...

_"...17

6..dnalytical

determinations ....ï...

...17

6.1. Total phenolic

content.

...17

6.2.Total flavonoids

content.

...17

7. Antioxidant

assays.

..."...18

7.1. Scavenging activity ag4lnst the DPPH

radical.,

..."...18

18

;l

Chapter

III:

Resttltk

and Discussion

l.

Moisture'confent....

...19

2.

Plackett-Burman Experimental design

of plant

extracts

...19

2:1. Statistical screening of important factors influencing

extraction..

...19

2.2. Global model and lack of

fit.

...,...21

2.2.l..Analysis

of

variance

..."..2I

2.3. Effects of independent factors on phenolic extraction

factors...

...22

(4)

Table

of

optimi2ation...,..,...

.",,.24

3.1. Estimated effects of factors on extraction

phenolic.

...24

3.2.

Fitfing

the model and optimal

condition

...,...27

3.3. Analysis of

variance...

...,...27

3.4. Several factors affecting antioxidants

activity....

...28

3.5. Validation and verification of predictive

mode1...

...29

4. Comparison

UAE

with

other

extraction

method..,..,,...,...,..

...29

Conclusion

...33

Bibliographical

references

Appendix

sC ,t {7

(5)

design was used

for the

optimization

of extraction parameters

in terms of

total phenolic contents, total flavonoid and

antioxidant activities. _{Result suggested the}_optimal_{conditions obtained}_{by FFD}

_{for UAE}

_as

follows: acetone

concentration

l}Yo,pH

(a)

and solid

to

solvent

_{l/50 glmL.In}

the optimal

conditionso

the

experimental

_total

phenolic contents

were

5066.99

mg

GAE/100

g,

total

flavonoid were 584,18 mg EQ/100 g and antioxidant

activity

of

89.31 o/o. Frnally,compared

proposed technique

to

_{conventional extraction methods and}

_MAE,

_the

_UAE

_provided_higher extraotion efficiency and offered many advantages.

Keywords

Ziziphu lottts; phwolic _{compounds; antioxidant}_{activities; Plaoket-Burman}_{design; fractional}_factorial

design; ultrasound-assisted _exhaction;_{optimization.}

Résumé

Laprérente éfude porte sur I'extraction des composés _{phénoliquesde Ziziphus}_îotus.Le

plan expérimental de Plackett-Burrnan et le plan factoriel fractionnairei 27'3 aété adopté pour

I'optimisation des paramètres d'extraction en composés phénoliques tôtaux, des flavonoides

totaux et d'activité antioxydante. Le _{résultat a suggére que les conditions optimales obtenues}

par FFD pour EAU comme suit : concentration en acétone l0%, pH (4), solide en solvant

l/50

g

I

_{mL. Dans les conditions optimales, le oontenu phénolique}_total_{obtenu expérimentalement}

est de 5066.99 mg GAE

_I

lA0 g, flavonoide total dst de 584.18 rng

Ee

/

100 g er pourcentage

d'inhibition _{de 89.31% du radical}_{DPPH*. Enfin,}_{en comparant}_EAU_{par rapport à la méthode}

d'extraction conventionnelle et au EAM, la technique EAU montre un rendement d'extraçtion plus élevé et de nombreux avantages.

Læs mots clé

Ziziphus latus

_i

composés phénoliques

_i

activité antioxydant ; plan plackett_Bunnan _{; plan}factonielle fractionnaire _;exbaction assisté par _{ultrasons ;}optimisation

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