• Aucun résultat trouvé

European Mycoplasma Conference London 2019

N/A
N/A
Info
Télécharger
Protected

Academic year: 2021

Partager "European Mycoplasma Conference London 2019"

Copied!
3
0
0

Chargement.... (Voir le texte intégral maintenant)

Texte intégral

(1)

1

European Mycoplasma Conference 18

th

-19

th

March 2019

Organising Committee

Authored by: Vicki Chalker & Brad Spiller

European Mycoplasma

Conference London 2019

(2)

2

Welcome to London!

We are thrilled you have travelled to join us to discuss mycoplasmas.

If you have any questions or need help please ask members of the

conference team (identified by stressed out appearance). We hope this

conference is valuable and you make many contacts for future fruitful

research and work.

We would like to thank our hosting organisations and sponsors for

supporting the meeting and our invited speakers who have travelled from

across the world to be with us.

We would like to thank the scientific committee for assisting in

programme design and abstract review, Gloria and Yvette for assisting

with administration and all the delegates for attending.

Wishing you a successful symposium

Vicki Chalker, Brad Spiller & the Organising Committee

London 2019

“

Imagination is more important than knowledge” Albert Einstein

“Sharing knowledge seeds imagination” Vicki Chalker

(3)

55

Abstract 42 Use of fluorescence expression tools for the comparative analysis of the interactions of Mycoplasma mycoides and Mycoplasma bovis with bovine cells Lucía Manso-Silván, Tiffany Bonnefois, Olivia Cadamuro, François Thiaucourt, Valérie Rodrigues, Philippe Totté CIRAD, UMR ASTRE, F-34398 Montpellier, France. INRA, UMR1309 ASTRE, F-34398 Montpellier, France. Background: Fluorescence expression systems adapted to the analysis of host-mycoplasma interactions were recently developed. The aim of this work was to apply them to study the colonisation and persistence capabilities of the bovine pathogens Mycoplasma mycoides subsp. mycoides (Mmm) and Mycoplasma bovis in bovine cells.

Methods. Mini-transposons affording high-level expression of GFP2, mCherry, mKO2 and mNeonGreen were used to mark Mmm and M. bovis strains. The resulting fluorescent mutants were characterised by epifluorescence microscopy and fluorimetry and the sites of transposon insertion were identified by sequencing. Interactions of mNeonGreen mutants with bovine cells were analysed using flow cytometry and confocal microscopy. Results: The production, selection and characterisation of fluorescent clones were straightforward and compatible with the production of fluorescent mutant banks. M. bovis presented much higher adhesion, invasion and proliferation capacities than Mmm in culture with non-phagocytic cells and showed increased resistance to elimination by macrophages. Conclusion: The remarkable differences in the invasion and persistence capabilities of Mmm and M. bovis observed here are in agreement with the pathogenesis of the infections caused by these mycoplasmas. mNeonGreen fluorescent mutants have proven extremely useful for analysis of mycoplasma-host cell interactions. Furthermore, the fluorescence expression tools used in this study offer innumerable perspectives for the functional analysis of a wide range of mycoplasma species both in vitro and in vivo.

Références

Télécharger maintenant ( PDF - 3 Page - 1.15 MB )

Documents relatifs

Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia

As shown in Figure 1, an increase in the percentages of CD4 + T cells and the prolifera- tion (i.e., growth index above 2) was detected but only in stimulated cultures prepared

Genotyping of Mycoplasma mycoides subsp. mycoides SC by multilocus sequence analysis allows molecular epidemiology of contagious bovine pleuropneumonia

The use of a technique based on sequencing multiple loci, designated multilocus sequence analysis (MLSA), allowed the identification of 15 dif- ferent allelic profiles within

Development of fluorescence expression tools to study host-mycoplasma interactions and validation in two distant mycoplasma clades

The level of fluorescence conferred to the mycoplasmas should be sufficient to enable their direct detec- tion inside host cells using affordable techniques such as confocal

Characterization of Free Exopolysaccharides Secreted by Mycoplasma mycoides Subsp. mycoides

We finally conducted in silico analyses of Mmm genes potentially involved in polysaccharide biosynthesis and proposed candidate pathways that might account for the

Draft genome sequences of Mycoplasma alkalescens, Mycoplasma arginini, and Mycoplasma bovigenitalium, three species with equivocal pathogenic status for cattle

Lucía Manso-Silván, a,b Florence Tardy, c,d Eric Baranowski, e,f Aurélien Barré, g Alain Blanchard, h,i Marc Breton, h,i Carole Couture, h,i Christine Citti, e,f Emilie

Draft genome sequences of Mycoplasma auris, Mycoplasma arginini, and Mycoplasma yeatsii, two species of the ear canal of Caprinae

INRA, UMR 1225, IHAP, Toulouse, France a ; Université de Toulouse, INP, ENVT, UMR 1225, IHAP, Toulouse, France b ; Université de Bordeaux, Centre de Bioinformatique et

Study of Mycoplasma mycoides subsp. Mycoides exopolysaccharides

The aim of this study was to develop a suitable methodology to produce and purify Mmm EPS, to measure their carbohydrate content and to compare them biochemically to the

Evolutionary history of contagious bovine pleuropneumonia using next generation sequencing of mycoplasma mycoides subsp. mycoides "small colony"

robust phylogeny for this pathogen, establish when the most recent common ancestor of all MmmSC strains appeared, determine whether CBPP existed in Africa or, more likely, it