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(1)

2 CONTENTS

REMERCIEMENTS... 4

RÉSUMÉ... 5

ABSTRACT... 6

I. INTRODUCTION ... 7

1. MULTIDISCIPLINARY APPROACH... 7

1.1. SPATIAL ECOLOGY... 7

1.2. INVASION ECOLOGY... 8

1.3. TOOLS... 9

2. SPATIAL AND INVASION ENTOMOLOGY... 14

3. STUDY BEETLE:IPS TYPOGRAPHUS... 15

3.1. GENERAL DESCRIPTION... 15

3.2. AN INVADER?... 16

3.3. STUDY CASE... 17

II. RESULTS... 18

III. DISCUSSION ... 21

1. DISPERSAL... 21

2. PATHWAY AND RISK ANALYSIS... 22

2.1. ASSESSMENT OF THE RISK OF INTRODUCTION... 22

2.2. THE ASSESSMENT OF THE RISK OF SPREAD... 23

2.3. THE USE OF GENETIC TOOL FOR RISK ASSESSMENT... 23

3. PERSPECTIVES ... 24

IV. REFERENCES... 25

V. ARTICLE I: Occurrence of Ips typographus (Col., Scolytidae) along an urbanisation gradient in Brussels, Belgium. ... 33

ABSTRACT... 33

INTRODUCTION... 35

MATERIAL AND METHODS... 36

STUDY AREA... 36

DATA... 36

ANALYSIS... 37

RESULTS... 38

DISCUSSION... 38

ACKNOWLEDGMENTS... 41

REFERENCES... 41

VI. MANUSCRIPT I: Insect introductions related to coniferous round wood imports from Russia to Belgium. A pathway analysis. ... 41

ABSTRACT... 41

INTRODUCTION... 42

MATERIAL AND METHODS... 43

1. Data sources ... 43

2. Data correction and comparisons... 47

RESULTS... 49

1. Information on pathways ... 49

2. Comparative assessment... 49

3. Coniferous round wood imports to Belgium... 50

DISCUSSION AND CONCLUSIONS... 52

Potential invaders... 53

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3

High volumes... 53

Treatments... 53

Dispersion and establishment... 54

ACKNOWLEDGMENTS... 54

REFERENCES... 54

VII. MANUSCRIPT II: TESTING IPS TYPOGRAPHUS(COLEOPTERA: SCOLYTINAE) exotic strains introduction scenarios in Belgium... 57

ABSTRACT... 57

INTRODUCTION... 58

MATERIALS AND METHODS... 59

STUDY AREA... 59

MODEL... 60

ENVIRONMENTAL LAYER... 62

POPULATION LAYER... 64

SPREAD AND GROWTH PARAMETERS... 65

SCENARIOS AND ANALYSIS... 66

RESULTS... 66

DISCUSSION... 70

ACKNOWLEDGMENTS... 72

REFERENCES... 73

VIII. MANUSCRIPT III: Is it possible to differentiate eastern and western European populations of the spruce bark beetle, Ips typographus (Coleoptera: Scolytinae) based on genetic population structure?... 77

ABSTRACT... 77

INTRODUCTION... 78

MATERIALS AND METHODS... 79

Insect collection... 79

DNA sequencing... 80

Data analysis... 81

RESULTS... 81

DISCUSSION AND CONCLUSIONS... 83

ACKNOWLEDGMENTS... 85

REFERENCES... 85

A. APPENDIX A: Note on Ips duplicatus in Belgium...88

B. APPENDIX B: Genetic analyses: additional information ...91

C. APPENDIX C: Framework for invasion processes ...92

D. APPENDIX D:Pest risk assessment standard scheme ...93

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