Ouoba et al., J. Appl. Biosci. 2019 Molecular characterization of the main fungi associated to Bambara groundnut foliar diseases in Burkina Faso
Journal of Applied Biosciences 133: 13574 - 13583
ISSN 1997-5902
Molecular characterization of the main fungi associated to Bambara groundnut foliar diseases in
Burkina Faso
Adjima Ouoba1,2*, Elisabeth P. Zida3, Romain W. Soalla, 3, Martine Bangratz4,5, Palanga Essowè, Moussa N’Golo Konaté2, Hervé Nandkangré2 , Mahama Ouédraogo2 and Mahamadou Sawadogo1
1Laboratoire Biosciences, Equipe Génétique et Amélioration des Plantes, Université Ouaga I Pr Joseph Ki- Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso
2Laboratoire de Génétique et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), Centre National de la Recherche Scientifique et Technologique (CNRST), 04 BP 8645 Ouagadougou, Burkina Faso
3Laboratoire de Phytopathologie et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), Centre National de la Recherche Scientifique et Technologique (CNRST), 01 BP 476, Ouagadougou, Burkina Faso
4Institut de Recherche pour le Développement (IRD), 911 Avenue Agropolis, 34394 Montpellier, France
5Laboratoire Mixte International Patho-Bios, 01 BP 476, Ouagadougou, Burkina Faso
Original submitted in on 19th September 2018. Published online at www.m.elewa.org on 31st January 2019 https://dx.doi.org/10.4314/jab.v133i1.9
ABSTRACT
Objective: This study aims to update the database of fungi associated to Bambara groundnut foliar diseases in Burkina Faso using both molecular and morphological identification approaches.
Methodology and Results: In this study, molecular approach based on the sequencing of ITS (Internal Transcripted Spacer) region of fungi and morphological approach were used to identify the main fungi associated to Bambara groundnut foliar diseases. The study was performed with universal polymerase chain reaction (PCR) primer ITS1/ITS4. BlastN comparisons between 19 fungal isolates contigs of the 16 major fungi were produced by their DNA sequences assembly and GenBank sequences yielded identity scores of 99 to 100 % with all of them. The degrees of similarity between these contigs and the loci sequences of classified fungi in GenBank indicate that our fungal isolates are the same species with those in Genbank, particularly the first of the list show after the blastN. It is the first report of molecular characterization of the main fungi infecting Bambara groundnut in Burkina Faso.
Conclusion and Application of results: Nineteen fungi associated to Bambara groundnut foliar diseases were identified and can be taken as targets in varietal improvement of Bambara groundnut for resistance to fungal diseases in Burkina Faso.
Key words: Bambara groundnut, fungi, molecular characterization, PCR primer ITS1/ITS4, Burkina Faso.
