HAL Id: inserm-00334722
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induction.
Frédéric Michon, Loïc Forest, Elodie Collomb, Jacques Demongeot, Danielle
Dhouailly
To cite this version:
Frédéric Michon, Loïc Forest, Elodie Collomb, Jacques Demongeot, Danielle Dhouailly. BMP2 and BMP7 play antagonistic roles in feather induction.. Development (Cambridge, England), Company of Biologists, 2008, 135 (16), pp.2797-805. �10.1242/dev.018341�. �inserm-00334722�
HH30 HH30 BMP-2 Follistatin HH30 HH30 C D R1 R2 R1 R2 R2 R1 R3 R1 R3 R2 R3 R4 Id-1 HH30 E HH30 HH30 F Id-3 G Id-4
basic media + FGF-2 + BMP-7 + BMP-2 + BMP-7 % R F U ( 4 8 5 /5 3 5 n m ) FBS 0% FBS 10% + BMP-2 controls 0 20 40 60 80 100 120 50
HH29+ HH30 EIIIA HH30 BMP-2 HH30 C D R2 R1 R3 R4 R2 R1 R3 R4
0 20 40 60 80 100 t0 t24 t48 t72 % R a ti o t o ta l F ib ro n e c ti n / Act in 0 10 20 30 40 50 60 t0 t24 t48 t72 % R a ti o EI II A / n o rm to t F ib ro 0 10 20 30 40 50 60 70 Control BMP-2 BMP-7 % R a ti o EI II A / n o rm to t F ib ro A B C 0 20 40 60 80 100 120 140 Control BMP-2 BMP-7 % R a ti o α 4 -I n te g ri n / Act in D
A B
Hexagonal pattern (pteryla)
q (
q (cellcelldensity)density)
q = 0.11 q = 0.12 q = 1 q = 0.2 A B C D Stable spots without hexagonal patterning (semi apterium) Irregular hexagonal pattern (border of a pteryla) No spot at all (apterium)
600µg/ml 200µg/ml 50µg/ml
A B C
5 rows of placodes hexagonal patterning placode formation homogeneous epidermis preplacodal epidermis BMP-7 BMP-7 BMP-7 X β-cat FGF-2 BMP-2 EIIIA EIIIA Follistatin Notch pathway e p id e rmi s d e rm is BMP-2 BMP-7 BMP-4 BMP-7 Notch pathway Integrins follicular domain identity stabilized dermal condensation unstabilized dermal condensation cell aggregates homogeneous dense dermis cell density modification migration
migration aggregationaggregation midline midline stabilization stabilization aggr aggr prolif prolif prolif
prolif prolifprolif
migr
migr migrmigr prolif prolif migr migr migr migr aggr aggr proliferation proliferation Wnt chemotaxis dermal cell migration BMP-2 follicular domain delimitation
