Characterization and comparison of French and Brazilian Potato virus Y (PVY) isolates collected from PVY-susceptible or -resistant tobacco plants carrying the recessive resistance gene va

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Characterization and comparison of French and Brazilian Potato virus Y (PVY) isolates collected from

PVY-susceptible or -resistant tobacco plants carrying the recessive resistance gene va

Christelle Lacroix, L. Glais, Camille Kerlan, C. Charlier, Bérenger Janzac, C. Laurencetti, F. Mornett, B. Cailleteau, J.L. Verrier, Emmanuel Jacquot

To cite this version:

Christelle Lacroix, L. Glais, Camille Kerlan, C. Charlier, Bérenger Janzac, et al.. Characterization and comparison of French and Brazilian Potato virus Y (PVY) isolates collected from PVY-susceptible or -resistant tobacco plants carrying the recessive resistance gene va. 2012 CORESTA Congress, Sep 2012, Sapporo, Japan. �hal-02805898�

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Characterization of

French and Brazilian PVY isolates

from susceptible or resistant

tobacco plants

LACROIX C

LACROIX C1212_{, GLAIS L.}_{, GLAIS L.}77_{, KERLAN C.}_{, KERLAN C.}11_,_,

CHARLIER C.

CHARLIER C.55_{, JANZAC B.}_{, JANZAC B.}1212_{, LORENCETTI C}_{, LORENCETTI C}66_,_,

MORNET F

MORNET F44_{, CAILLETEAU B.}_{, CAILLETEAU B.}33_,_,

VERRIER J.

VERRIER J.--L.L.33_{, JACQUOT E.}_{, JACQUOT E.}11

(3)

A collaborative project

1.

1. INRA INRA -- InstitutInstitut National de la National de la RechercheRecherche

Agronomique

Agronomique 2.

2. ARN ARN -- Association pour la Association pour la RechercheRecherche sursur les les

Nicotian

Nicotianééeses

3.

3. InstitutInstitut dudu TabacTabac, Imperial Tobacco, Imperial Tobacco 4.

4. ANITTA ANITTA -- Ass. Ass. NationaleNationale InterprofessionnelleInterprofessionnelle

et Technique

et Technique dudu TabacTabac

5.

5. UNISC UNISC -- UniversidadeUniversidade de Santa Cruz do de Santa Cruz do SulSul 6.

6. AOB AOB -- Alliance One Alliance One BrasilBrasil 7.

7. FN3PT FN3PT –– FFééddéérationration NationaleNationale des des ProducteursProducteurs

de Plants de

de Plants de PommesPommes de Terrede Terre

8.

(4)

The

same

team

could

run

parallel

PVY

_PVY

studies

_studies

n

Brazil

n

(5)

Potato

virus Y (PVY)

n

Potyvirus

genus

•

• Filamentous

Filamentous

flexuous

particle

•

180

180 species

species

•

• PVY = type

PVY = type

-

member

n

Aphid

transmitted

•

• Non persistent

Non persistent

•

• 40 species potential vectors

40 species potential vectors

HC-Pro P3 CI NIa NIb CP

P1 3’ 5’ VPg ₍₍_A)_A) n n ss RNA (+) 10 Kb 100 nm 100 nm

(6)

PVY Host Plants

Potato Tomato Pepper

Tobacco

Petunia _{Black nightshade}

Principally Solanaceae

C ro p s rn am en ta ls ee d s

(7)

PVY in Tobacco

n

Worldwide

distributed

•

• Nearly

Nearly

all

tobacco

crops

n

n More More particularlyparticularly Europe, Europe, EasternEastern AsiaAsia, , alsoalso

in South

in South AmericaAmerica and and SouthernSouthern AfricaAfrica

n

n Important Important economiceconomic impactimpact

n

Main

means

of control

•

• Genetic

Genetic

resistance

of cultivars

n

n The The onlyonly resistanceresistance developeddeveloped : : «« vava »» n

n SomeSome strainsstrains overcomeovercome thisthis resistanceresistance,, n

(8)

Questions to

answer

_answer

n

What

is

the

prevalence

of PVY in

tobacco

crops

,

compared

to

other

viruses

•

• SymptomaticSymptomatic / / symptomelesssymptomeless plantsplants

n

How diverse are PVY

isolates

found

on

tobacco

•

• WithWith regard to area of regard to area of originorigin •

• WithWith regard to the partial regard to the partial resistanceresistance (va (va vava) or ) or suceptibility

(9)

A 4

-

_-

step

_step

process

_process

1.

Field

surveys

•

• HarvestedHarvested leavesleaves

n

n FromFrom symptomaticsymptomatic or or syptomelesssyptomeless plantsplants

•

• More More frequentlyfrequently symptomaticsymptomatic plantsplants

n

n France, 2007, France, 2007, BrazilBrazil, 2009, 2009--10.10.

2.

Identification of viral

species

•

• ImmunologicalImmunological (ELISA) tests(ELISA) tests

3.

PVY

isolates

: virulence

study

•

• AbilityAbility to to overcomeovercome or not or not severalseveral «« vava »» alleles

alleles –– tests in the tests in the greenhousegreenhouse..

4.

Treatment

of

results

with

regard to

•

• GeographicGeographic originsorigins •

• Resistance / Resistance / susceptibilitysusceptibility of the original host of the original host cultivar

(10)

Identification of viral species

n

Origin of

antisera

•

• PVY:

PVY:

polyclonal

, INRA

-

Rennes

-

FN3PT

•

• CMV:

CMV:

polyclonal

, INRA

Avignon

•

• AMV:

AMV:

polyclonal

, LCA, Bordeaux, France

•

• TMV:

TMV:

polyclonal

, LCA, Bordeaux, France

•

• TVMV:

TVMV:

polyclonal

,

Sediag

, France

n

Double antibody sandwich enzyme

linked

(11)

ELISA tests on PVY

isolates

_isolates

n

(12)

Field

survey

_survey

,

_,

Brazil

_Brazil

2009

₂₀₀₉

-

_-

10 ₁₀

n

397

397 samples

samples

from

R & S cultivars

0 80 160 PAR SCW RGM RGC S R va va Va Va

(13)

X X X X X X X X X _X X X X X X X X X more than 200 0 100 to 200 0 to 100 Number of producers : X Areas of sampling :

Veinal necrosis Mosaïc No

symptoms Particular

symptoms

Field

survey

_survey

France 2007

_{France 2007}

556 samples

North: only resistant cvs S+: 471 Symptomless : 85

South: res. and susceptible cultivars

(14)

0% 50% 100% France 2007 Brazil 2009 S-, no detection S-, some virus S+, no detection S+, some virus

Virus

detection

_detection

and

_and

symptoms

_symptoms

n

n BrazilBrazil: a : a significantsignificant percentagepercentage of plants of plants thatthat showedshowed S+ symptoms S- no symptoms

(15)

Identified

viral

_viral

species

_species

n

> 100 : Mixed infections

common

in

both

countries

0 50 100 150 France Brazil

%

d

e

te

c

te

d

TVMV TMV CMV AMV PVY

(16)

ELISA tests on PVY

isolates

_isolates

n

In

south

of

Brazil

, the

most

frequent

serotype

on

tobacco

cannot

be

assigned

to

the

classical

PVY

OO

_{/ PVY}

NN

_serogroups

(17)

Virulence of PVY

isolates

_isolates

n

n SubSub--samplesample of of isolatesisolates representativerepresentative of of

geographic

geographic originorigin and host and host resistanceresistance (Va or va)(Va or va)

•

• France: 86, France: 86, BrazilBrazil :29 :29

n

n InoculatedInoculated in the in the greenhousegreenhouse on:on:

•

• Susceptible standard MN944 (Va Susceptible standard MN944 (Va VaVa)) •

• VAM (vaVAM (va0 0 _va_va00₎₎

•

• WislicaWislica: (va: (va1 1 _va_va11₎₎

•

• PBD6: (vaPBD6: (va2 2 _va_va22₎₎ n

n 4 plants of 4 plants of eacheach line + line + healthyhealthy controlcontrol n

n ResultsResults basedbased on ELISA. on ELISA.

•

(18)

Virulence versus original host

Host tobacco plant from which collected S: Va. , R: vava 0% 50% 100% S R S R France Brazil Unable to overcome va Some ability Overcomes va0-1-2 V ir u le n ce a ss e ss e d in t h e g re e n h o u se 43 43 18 11

(19)

Treatment of results, Brazil

n

n Probability for a plant to be infected by PVY Probability for a plant to be infected by PVY

•

• smaller in smaller in vava--resistant tobacco hosts (P<0.01)resistant tobacco hosts (P<0.01)

n

n Distribution of Distribution of serotypesserotypes

•

• dependant on area (P<0.001)dependant on area (P<0.001) •

• PVY and YPVY and YU U _{isolates more frequent in Santa}_{isolates more frequent in Santa}_Catarina_Catarina

West (P<0.01)

•

• High % of YHigh % of YNN _{isolates in the northern area (PAR, SCW).}_{isolates in the northern area (PAR, SCW).}

Connections between potato and tobacco

compartments?

n

n Among the 29 isolates studied for virulence, the Among the 29 isolates studied for virulence, the

20

(20)

Treatment of results, France

n

Probability for a plant to be infected by

PVY

•

• smaller in smaller in vava--resistant tobacco hosts (P=0.02)resistant tobacco hosts (P=0.02)

n

Distribution of

serotypes

•

• dependant on area dependant on area •

• % of PVY% of PVYO/CO/C _{higher in south than in NE}_{higher in south than in NE}

(P=0.013) and NW (P<0.01)

•

• % of PVY% of PVYNN _{higher in NE (P<0.01) and}_{higher in NE (P<0.01) and}

NW(P=0.019) than in South

n

PVY

U U

(non O non N) was more frequent on

“

(21)

Conclusions

n

PVY

is

the

most

prevalent

virus on

tobacco

,

both

in France and

Brazil

.

n

In

south

of

Brazil

, the

most

frequent

serotype

cannot

be

assigned

to the

classical

PVY

OO

_{/ PVY}

NN

_serogroups

n

In

both

countries

•

• isolatesisolates fromfrom «« vava »» cultivars show a cultivars show a muchmuch broader

broader virulence virulence thanthan thosethose fromfrom «« VaVa »» plants.

plants.

•

• «« va va vava »» plants plants lessless oftenoften infectedinfected by PVYby PVY •

• SameSame trends trends observedobserved in in differentdifferent agronomicagronomic and