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Submitted on 16 Dec 2019
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Central pattern generator-driven efference copy couples eye movements with forelimb locomotion in mice
F França de Barros, C Taillebuis, M. Manuel, H. Bras, D Combes, François Lambert, M. Beraneck
To cite this version:
F França de Barros, C Taillebuis, M. Manuel, H. Bras, D Combes, et al.. Central pattern generator- driven efference copy couples eye movements with forelimb locomotion in mice. 2019 annual meeting Society for Neurosciences, Oct 2019, Chicago, United States. �hal-02413495�
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Presentation 062.11 1-INCC UMR 8002, 2-SPPIN UMR 8003, CNRS, Paris, France; 3-INT UMR 7289, CNRS, Marseille, France;
4-INCIA UMR 5287, CNRS, Bordeaux, France; 5-Univ. de Paris, France; 6-Univ. Bordeaux, France
Central pattern generator-driven efference copy
couples eye movements with forelimb locomotion in mice
F. França de Barros
1,5, C. Taillebuis
1,5,6, M. Manuel
2,5, H. Bras
3, D. Combes
4,6, F. M. Lambert
4,6, M. Beraneck
1,5Eye movements during treadmill locomotion in precollicular premammillary decerebrated
Acknowledgments
Work funded by the French entities: Agence Nationale de la Recherche (ANR) and the Centre National de la Recherche
Scientifique (CNRS).
We also thank Université de Paris and Bordeaux and the technical support provided by Patrice Jegouzo.
5 s 4 Hz
4Hz
5 s
-1.0 0.0 +1.0
Time(s)
-1.0 0.0 +1.0
Time(s) C8
Right
CN VI Left
CN VI
LeftC8 Right
C8
LeftL5 Right
L5
Right CN VI CN VILeft
Right C8 LeftC8
Right L5
n= 10 cycles
n= 10 cycles S1
Right
CN VI Left
CN VI
Right
C8 Left
C8
Right CN VI CN VILeft Right
C8 LeftC8 LeftL5
Sacral S1 stimulation - Lumbar CPG activation
1. The extra-ocular activity is coupled to fictive locomotion
Cervical C8 stimulation - Cervical CPG activation
Ex vivo recordings of the eye’s motor nuclei and locomo- tor CPGs in brainstem-spinal cord preparations of
neonatal mice
Ctrl Section 0
50 100
0.0 0.2 0.4 0.6 0.8
0.0 0.2 0.4 0.6 0.8 1.0
Ctrl Section
ns
5 s 5 s 5 s
r²=0.96
r²=0.99
S1
Control After full-cut midthoracic
S1 stim. S1 stim. C8 stim.
Right
CN VI Left
CN VI
LeftC8 C8
CN VILeft LeftC8 Right CN VI
LeftL2 LeftL2
Absolute Latency(ms)
CN VI vs C8
Ipsilateral Contralateral C8 Frequency(Hz)
CN VI Frequency(Hz)
Lumbar CPG activation Lumbar CPG activation Cervical CPG activation
2. Blocking the cervical locomotor network removes the spino-extraocular coupling
3. The cervical locomotor network is sufficient to produce the spino-extraocular coupling
Control 0 Ca2+ Washout
-In dark
- Head-fixed
-Motorized treadmill
1. Locomotion: treadmill induced a trot-like pattern.
0 2 4 6 8 10
0 2 4 6 8 10
Forepaw IF(Hz)
HindpawIF(Hz)
0 2 4 6 8 10
0 2 4 6 8 10
Forepaw IF(Hz)
HindpawIF(Hz)
R=0.4715 R=0.2333
R=0.7746 R=0.2620 R=-0.1494
180 0
2. Eyes: conjugated eye movements are correlated and in phase
0 2 4 6 8 10
0 2 4 6 8 10
Forepaw IF(Hz)
EyeHorizontalIF(Hz)
R= 0.4224
0 2 4 6 8 10
0 2 4 6 8 10
Forepaw IF(Hz)
EyeHorizontalIF(Hz)
0 2 4 6 8 10
0 2 4 6 8 10
Hind paw IF(Hz)
EyeHorizontalIF(Hz)
3. Coupling characteristics between limbs and eyes
0
0 2 4 6 180
0 2 4 6
Right eye IF(Hz)
LefteyeIF(Hz)
R=0.8871
3 2.25 1.5 0.75
180 0
Forepaw vs eye horizontal Right eye vs left eye
Forepaw vs hind paw
0
180
1s
Amplitude(°) Velocity(°/s)
Rational
Efference copies are neural replicas of motor outputs used to anticipate the sensory consequences of a self-generated motor action.
They are responsible for the coordination of motor beha- viors by generating intrinsic feed-forward signals that directly connect a local motor circuitry with another local circuitry involved in another motor behaviour.
An example of a predictive motor efference copy is the signal originating from the locomotor spinal CPG that is simultaneously responsible for tail ondulation as well as for driving eye movements during swimming in the Xenopus tadpole.
Study whether the CPG-driven predictive signal cou- pling eye movements to locomotion is preserved in mammals.
Credit: F.Lambert
1. Ex vivo: rhythmic coupled discharge was found between limb and abducens motor nerve activity during fictive
locomotion events elicited by both electrical and pharmacological stimulation.
2. In vivo: in absecbe of visual and vestibular inputs, conju- gated eye movements are coupled to locomotion were recorded, suggesting that the efference copy particpiates in gaze stabilization during viogorus locomotion.
3. Anatomy: rabies virus injection revealed ipsilateral monosynaptic ascending spinal connection from the cervi- cal with abducens motoneurons.
Conclusion Aim
Neuronal substrates of the spino-extraocular pathway
55h
infection Monosynaptic tracing Migration
7547 µm ±1798 Rabies virus in
lateral rectus muscle
nVI nVII g7
*
50 µm 50 µm
RVChAT Hoescht
RVChAT Hoescht
RVChAT
ChATRV Hoescht
cc
cc cc
50 µm 50 µm
C1-2
C3-4
C5-6
C7-8
C7-8
T1-2
nVII
0 5 10 15
0 10 20 30 40 50
Infected MNs
InfectedINs
LOCOMOTION
128 130 132 134 136 138 140 142 144 146 148 150 152 154 164156 158 160 162
Left
Forepaw Stance Swing
5sec
5deg Left eye
Hor pos
2deg 200ms Right eye
Hor pos
Left Right Treadmill ON
10cm/s
5deg
Injection of Rabies virus in the lateral rectus of adult mice:
1. Infected (RV+) motorneurons (ChAT labelling) in the abducens nucleus(nVI) ipsilateral to the injection.
2. RV+ interneurons are present all along the cervical (7547µm ± 1798).
3. On average, 1 infected MN in the nVI affects 6 INs in the cervical.
4. C7 shows an homogeneous bilateral infection; C2-C6 and C8 infected mostly ipsilaterally (68%).
C1 C2 C3 C4 C5 C6 C7 C8 0
2 4 6 8 10
InfectedINs Contralateral
Ipsilateral
