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Dysregulated calcium homeostasis prevents plasma membrane repair in Anoctamin 5/TMEM16E-deficient patient muscle cells

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Academic year: 2021

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Fig. 1 ANO5 de fi cit does not slow growth and differentiation of patient myoblasts. a Chromatogram for the sequence of the genomic DNA region of patient (middle and bottom) and healthy (top) myoblasts examining the mutation of the ANO5 gene
Fig. 2 ANO5 is an ER protein that is required for muscle PMR. a, b Glass bead injury assay for healthy and patient myoblasts
Fig. 3 Effect of injury on the ANO5 distribution and ER structure. a Images of healthy myoblasts expressing ANO5-GFP and RFP-KDEL are injured (at site marked by the arrow) by laser beam
Fig. 5 ANO5 de fi cit causes [Ca 2+ ] c overload in injured cells without affecting ER stress response
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