HAL Id: jpa-00224296
https://hal.archives-ouvertes.fr/jpa-00224296
Submitted on 1 Jan 1984
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DIRECT DETERMINATION OF DENSITY AND LOCATION OF WATER ASSOCIATED WITH TRANSFER RNA AND OF THE CONFORMATION OF THE MACROMOLECULE AS A FUNCTION OF
SALT IN THE SOLUTION
G. Zaccai
To cite this version:
G. Zaccai. DIRECT DETERMINATION OF DENSITY AND LOCATION OF WATER ASSOCI- ATED WITH TRANSFER RNA AND OF THE CONFORMATION OF THE MACROMOLECULE AS A FUNCTION OF SALT IN THE SOLUTION. Journal de Physique Colloques, 1984, 45 (C7), pp.C7-265-C7-265. �10.1051/jphyscol:1984731�. �jpa-00224296�
JOURNAL DE PHYSIQUE
Colloque C7, suppl6ment au n09, Tome 45, septembre 1984 page C7-265
DIRECT DETERMINATION OF DENSITY AND LOCATION OF WATER ASSOCIATED WITH TRANSFER RNA AND OF THE CONFORMATION OF THE MACROMOLECULE AS A
FUNCTION OF SALT IN THE SOLUTION
G. Zaccai
I n s t i t u t Max von Laue-PauZ Langevin, 156 X , 38042 GrenobZe Cedex, Frmee
By using a contrast variation approach in small angle neutron scattering experiments, the microenvironment around yeast ~ R N was characterised quantitatively by its A ~ ~ ~ excess density over bulk solvent and radius of gyration about the centre of mass of the macromolecule. The radius of gyration of the macromolecule alone was also determined. Experiments were performed in a number of different salt conditions.
In NaCl and KC1 solutions between 0.1 M and 1 M, the conformation of the tRNA macromolecule is similar to that observed in single crystal X-ray studies. The microenvironment, on the other hand, varies in its structure both with the nature of the salt and its concentration. This work is described in detail by Li et al,, Biochemistry, 2, 4380-4388, 1983. In solutions of N(CH3)4C1 (TMA.Cl), the tRNA macromolecule remains in an extended conformation, similar to its low salt structure, in the entire concentration range studied (up to 2M TMA.Cl). When TPJA.Cl is mixed with NaCl or MaC12, there is competition between the different cations to "structure" the macromolecule (Xian and Zaccai, manuscript in prepara- tion).
Article published online by EDP Sciences and available at http://dx.doi.org/10.1051/jphyscol:1984731
