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Crystal Structure of an Integron Gene Cassette-Associated Protein from Vibrio cholerae Identifies a Cationic Drug-Binding Module

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Academic year: 2021

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Figure 2. Phylogenetic tree of Cass2 sequence with evolutionary distances. Bootstrap values $80% are shown over the internodes for 36 sequence homologues
Figure 3. Multiple alignment of Cass2 and sequence homologs. Cass2 sequence is aligned with representative strains from Vibrio: Vv, Vibrio vulnificus CMCP6 (AE016795.2); Vh, Vibrio harveyii ATCC-BAA-1116 (YP_001448888.1); VcM, Vibrio cholerae MZO-2 (ZP_019
Figure 5. Structural homologs of Cass2. A) Stereo superposition (wall-eyed view) of Cass2 crystal structure (red), C-terminal domain of Rob transcription factor, E.coli (purple, PDB 1D5Y) [26], gyrase inhibitory protein GyrI, E.coli (orange, PDB 1JYH) [28]
Table 1. Ligand binding affinities (K D ( m M)) of Cass2 and BmrR for cationic compounds.
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