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(1)Optimisation du traitement du cancer du sein Triple-Négatif : développement des modèles de culture cellulaire en trois dimensions, efficacité de l’Olaparib (anti-PARP1) en combinaison avec la radiothérapie et chimiorésistance instaurée par les protéines Multi Drug Résistance Clemence Dubois. To cite this version: Clemence Dubois. Optimisation du traitement du cancer du sein Triple-Négatif : développement des modèles de culture cellulaire en trois dimensions, efficacité de l’Olaparib (anti-PARP1) en combinaison avec la radiothérapie et chimiorésistance instaurée par les protéines Multi Drug Résistance. Cancer. Université Clermont Auvergne, 2018. Français. �NNT : 2018CLFAS018�. �tel-02166394�. HAL Id: tel-02166394 https://tel.archives-ouvertes.fr/tel-02166394 Submitted on 26 Jun 2019. HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés..

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Figure

Figure 1: Profile of spheroid formation with the SUM1315 cell line in light microscopy
Figure 3: Parameter determination for MDA-MB-231 cell line spheroid formation.  For MDA-MB-231 cell line spheroid  formation, 50 to 10000 cells were seeded in 96-wells microplates (“ULA” for Ultra Low Attachment, Corning®) at Day 0 and 2% Geltrex®
Figure 4: 3D topology of SUM1315 and MDA-MB-231 spheroids by Scanning Electron Microscopy (SEM)
Figure 5: Ultrastructure of SUM1315 and MDA-MB-231 spheroids by Transmission Electron Microscopy (TEM)
+3

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