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Genome-wide approaches to elucidate evolution of sex determination systems and sex determining genes in fish

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HAL Id: hal-02733991

https://hal.inrae.fr/hal-02733991

Submitted on 2 Jun 2020

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Genome-wide approaches to elucidate evolution of sex determination systems and sex determining genes in fish

Qiaowei Pan, Romain Feron, Elodie Jouanno, Ming Wen, Boudjema Imarazene, Jennifer Anderson, Verena A. Kottler, Alvaro Santacruz Roco,

Kang Du, Suzanne Kneitz, et al.

To cite this version:

Qiaowei Pan, Romain Feron, Elodie Jouanno, Ming Wen, Boudjema Imarazene, et al.. Genome-wide approaches to elucidate evolution of sex determination systems and sex determining genes in fish. 8.

International Symposium on the Biology of Vertebrate Sex Determination, Apr 2018, Kona (Hawaii), United States. �hal-02733991�

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EIGHTH INTERNATIONAL SYMPOSIUM ON THE BIOLOGY

OF VERTEBRATE SEX DETERMINATION

. '

A11ril 16-20, 2018 KONA, HAWAII

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EIGHTH INTERNATIONAL SYMPOSIUM ON THE BIOLOGY OF VERTEBRATE SEX DETERMINATION

16-20, APRIL 2018

King Kamehameha's Kona Beach Hotel Kailua-Kona, Hawaii

Organizers Richard Behringer

University of Texas MD Anderson Cancer Center Houston, Texas

Blanche Capel Duke University , Durham, North Carolina

Martin Cohn

Howard Hughes Medical lnstitute University of Florida

Gainsville, Florida Monika Ward

University of Hawaii at Manoa Honolulu, Hawaii

Cover: Statue of Ardhanarishvara, the hait-male, half-female form of Shiva, lndia 111h

century A.D.

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GENOME-WIDE APPROACHES TO ELUCIDATE EVOLUTION OF SEX DETERMINATION SYSTEMS AND SEX DETERMINING GENES IN FISH Qiaowei Pan1, Romain Feron1, Elodie Jouanno1, Min Wen1, Boudjema lmarazene1,

Elodie Jouanno1, Jennifer Anderson1, Verena Kottler2, Alvaro Santacruz Roco2, Kang Du2, Suzanne Kneitz2, Zhongwei Wang2, Mateus Adolfi2, Catherine Wilson3, Braedan

McCluskey3 , Angel Amores3, Thomas Desvigne3, Christophe Klopp4, Céline Lopez- Roques5, Olivier Chabrol5, Cecile Donnadieu5 , Hugues Parrinello6 , Laurent Journot6,

Amaury Herpin1, John H. Postlethwait3, Manfred Schartl2, Yann Guiguen1.

11NRA, Fish Physiology and Genomics laboratory, 35042 Rennes, France. 2University of Wuerzburg, Physiological Chemistry, Biocenter, 97074 Wuerzburg, Germany.

3University of Oregon, lnstitute of Neuroscience, Eugene OR 97403, USA 41NRA, SIGENAE, UR875, INRA Auzeville, 31326 Castanet-Tolosan, France. 51NRA, GetPlage INRA Auzeville, 31326 Castanet-Tolosan, France. 6MGX (Montpellier Genomix), 34094

Montpellier Cedex 05, France

Fish show a great variety of sex determination mechanisms ranging from pure environmental (ESD) to pure genetic (GSD) with high variability of sex chromosome differentiation. Curiously, this variability does net follow any obvious phylogenetic pattern with transitions within closely related genera or even within species. To better understand the biological significance of sex determination diversity and the mechanisms driving sex chromosome evolution we attempted ta decipher the molecular basis of the primary sex determination mechanisms and the structure and genetic organization of sex chromosomes across a broad diversity (>40 species) of ray-finned fishes (Actinopterygii) and one cartilaginous fish (Chondrichthyes). On the one hand, we analysed a broad collection of species that represent major branches of the fish tree of life, and on the other hand, we focused on closely related species within some branches of the phylogenetic tree (Esociformes, Danioids, Poeciliids, Pangasiidae). We used a large array of genomics approaches including, restriction site-associated DNA (RAD) sequencing, pool- sequencing, transcriptomics and de novo whole genome sequencing to characterize sex determination systems, delineate sex-specific chromosomal regions and identify candidate sex determining genes. These strategies led ta the identification of many XX/XY or WZW simple monofactorial systems but also species with more complex sex- determination systems including species with a mix of GSD and ESD and species with potential polygenic systems. Sex-biased markers and whole genome sequences delineated the extent of recombinatlon suppression showing a wide range of sex- chromosome differentiation. Finally, novel master sex determination gene candidates were also identified in some species.

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