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Proteorhodopsine et hydrogénase https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-11-2

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Proteorhodopsine et hydrogénase

https://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-11-2

Schematic diagram of H2 production by the co-expression of proteorhodopsin and [NiFe]- hydrogenase in E. coli.

Proteorhodopsin transports protons across the membrane by absorption of light energy. Protons are transferred to the active site in the large subunit (L) of [NiFe]-hydrogenase in the periplasm and reduced to H2 by the addition of an electron, which is transferred thorough [Fe-S] clusters in the small subunit of [NiFe]-hydrogenase (S) and b-type cytochrome […] in the inner membrane.

Abbreviations: OM, outer membrane; PS, periplasmic space; IM, inner membrane.

Diagramme schématique de la production H2 par la co-expression de la protéorhodopsine et [NiFe]- hydrogenase dans E. coli *.

La protéorhodopsine transporte des protons à travers la membrane sous l’effet de l’absorption de l’énergie lumineuse. Les protons sont transférés sur le site actif dans la sous-unité (L) de l’hydrogenase [NiFe] et réduits en H2 par un électron transféré depuis un cytochrome b […] de la membrane interne par le cluster [Fe-S] dans la sous-unité (S) de l’hydrogenase [NiFe].

Abréviations : OM, membrane externe; PS, espace périplasmique; IM, membrane intérieure.

* Escherichia coli est un bacille de la famille des Enterobacteriaceae.

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