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‘Next generation’ biogeography: towards understanding the drivers of species diversification and persistence

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SUPPORTING INFORMATION

Appendix S1 RAD sequencing results for individuals of three restiad species, Restio capensis, Restio triticeus and Hypodiscus aristatus,

discussed in the main text. For each individual, the species identity is indicated, as are the number of Illumina sequencing reads obtained, the number of sequence clusters passing quality tests and the average and median sequencing depth (= coverage) of each quality cluster. Individual IDs refer to DNA IDs used in our laboratories. DNA or tissue samples for these specimens are available from the authors upon request.

Individual Species Reads Clusters Average seq. depth Median seq. depth

Ha.b17.107 Hypodiscus aristatus 4830434 351882 22 13

Ha.b17.109 Hypodiscus aristatus 4897493 272461 28 16

Rc.d13.81 Restio capensis 7670722 275002 36 20

Rc.d13.82 Restio capensis 7469682 312006 32 19

Rt.137 Restio triticeus 11328988 217529 65 32

Rt.138 Restio triticeus 10647337 215603 62 30

Published in "Journal of Biogeography doi: 10.1111/jbi.12076, 2013" which should be cited to refer to this work.

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