Implementing a networking platform to promote and enhance science and cooperation with Europe in food quality and safety

Session 5: The European Program – New science in traceability

IMPLEMENTING A NETWORKING PLATFORM

TO PROMOTE AND ENHANCE SCIENCE AND TECHNICAL

CO-OPERATION WITH EUROPE

IN FOOD QUALITY AND SAFETY

Food Traceability: the key to global market access

1st and 2nd of April 2008, Kuala Lumpur, Malaysia

Mathieu WEIL, Caroline BLACHE, Fabrice DAVRIEUX,

Didier MONTET, Marc LEBRUN, Dominique PALLET

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 2

IMPLEMENTING A NETWORKING PLATFORM

TO PROMOTE AND ENHANCE SCIENCE AND TECHNICAL

CO-OPERATION WITH EUROPE

IN FOOD QUALITY AND SAFETY

CONTENTS

1. What is Cirad ?

2. Presentation of the ALCUE Food project

3. A few Cirad’s researches on traceability using innovative tools for

Food Quality and Safety

- Polymerase Chain Reaction – Denaturant Gel Gradient Electrophoresis

- Electronic nose

French Agricultural Research Centre

for International Development

working

working

for

for

developing

developing

countries and the

countries and the

French

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 4

Six strategic lines of research

Ecological intensification

Biomass energy in favour of communities in the South

Safe, varied food

Animal health and emerging diseases

Public policy, poverty and inequality

Relations between agriculture, environment,

nature and society

One operating principle: partnerships

Projects built and implemented hand-in-hand with

research and development players

•

•

Research

Research

organizations

organizations

and

and

universities

universities

• Professional

• Professional

organizations

organizations

and the

and the

private

private

sector

sector

• Public

• Public

authorities

authorities

and

and

donor

donor

agencies

agencies

• Local

• Local

authorities

authorities

•

•

NGOs

NGOs

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 6

3 Departments - 60 research units

1800 persons including 750 researchers

Metropolitan France: 430 researchers

Outside metropolitan France: 320 researchers

French overseas regions = 110

Other countries = 210, including 30 in international research centres

www.alcuefood.org

www.alcuefood.org

“From European fork to Latin American farm”: an innovative networking

platform for EU – LAC partnerships in food quality and safety R&D”

EC FP6 SSA Alcue-Food, 2005-08

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 8

MAIN GOAL

To strenghthen the EU-LAC R&D cooperation through the effective

involvement of LAC partners in european consortium on FQS

GENERAL OBJECTIVE

To implement a permanent

EU-LAC platform on FQS

To develop and

implement a

comprehensive

EU-LAC

information

system on FQS

issues

SPECIFIC OBJECTIVES

To define

EU-LAC stakes on

FQS and state

priorities on

elaboration of

joint R&D

projects

To enhance the

LAC capacity

building through

better use of the

existing EU-LAC

cooperation

instruments

To propose to EC

and/or european

consortia relevant

LAC "groups of

excellence" for

further involvment in

the new FP 6&7

instruments

KEY DATAS

Full project time : 36 months

Total human resources : 121 person months – 13 partners

4 EU Member States (France, Belgium, Spain, Portugal)

4 Latin American MERCOSUR countries (Argentina, Brazil, Chile, Uruguay)

Total Budget : 737 500 euros

EXPECTED RESULTS

Strenghthening of trade in Food Sector

Compliance of LAC Food Exports with EU regulations

PROJECT CUSTOMERS

All stakeholders of the food supply chain : producers, firms, consumer

associations, academic and research sector, policy makers, regulatory bodies

A total

food chain

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 10

Traceability

Traceability

was

was

identified

identified

as one of major

as one of major

priority

priority

topics

topics

(existence of a knowledge, existence of research activities …)

Organization of Workshops

and

Training sessions

Elaboration

of

joint projects

Data base

implementation

Information System

(Web portal)

Dissemination

activities

Project

activities

Field: Traceability in the food chain

Field: Traceability in the food chain

Organization of workshops and training sessions

Organization of workshops and training sessions

“Geo information in traceability systems, agricultural production systems and

environmental management : opportunities in regional development”

Campinas, Brazil, 27th of July 2005, 35 participants

“Recent advances on food traceability systems”

Santiago, Chile, 1st of August 2005, 95 participants

“Opportunities for EU-LAC co-operation on food traceability systems”

Santiago, Chile, 2-3 August 2005 (60 participants)

“Traceability systems along the meat chain”

Buenos Aires, 4th of August 2005 (60 participants)

“Strategic Technology Watch - applied to traceability, why and how to use

Internet efficiently”

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 12

Offer interface with INCO (southern cone) countries

to FP6 project (SSA PETER)

Facilitate integration of INCO partners in the EC top-up

procedure (joining Integrated Projects or Network of

Excellence)

Elaboration of new projects for the FP6/FP7

(ex: SSA OTAG -Geodecision to Track and Trace

Agricultural Production)

Elaboration of joint EU

Elaboration of joint EU

-

-

LAC Projects

LAC Projects

Field: Traceability in the food chain

Building a Database in traceability

between the two regions

for Key Stakeholders:

Producers, Entreprises and Traders

Scientists and Research Institutions

Consumer Groups and Policy Makers

Data base implementation

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 14

Information System (www.alcuefood.org)

Through the Information System / Web Portal

Through Workshops and Trainings

Through Participation in PETER:

- Disseminate European information to LAC/INCO countries

- Put forward LAC/INCO demands/contexts for better

adequation of traceability systems to the real needs

Dissemination activities

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 16

developped in Joint Research Unit QualiSud

- PCR DGGE (fishes)

- Electronic nose (dates)

- Near Infra Red Spectrometry (coffee and cocoa)

3. A few Cirad’s researches on traceability using

innovative tools for Food Quality and Safety

Microbial ecology to trace origin

by Polymerase Chain Reaction – Denaturant Gel Gradient Electrophoresis

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 18

New method PCR-DGGE

Food sample

Total DNA including

microbial DNA and

Food DNA

Mixture of DNA

amplicons from

species of

microorganisms

Extraction of total DNA

PCR Amplification of a ribosomal

DNA variable region by specific

primers

Comparative analysis of sequences and

determination of microbial markers of specific area

Separation of different

DNA by DGGE

Dendrogramme de 25 Obs. Moy. non pond. grpes associés

Percent disagreement AGB2P3-P AGB2P2-P AGB2P1-P AGB5P5-P AGB5P4-P AGB5P3-P AGB5P2-P AGB5P1-P AGB4P5-P AGB4P4-P AGB4P3-P AGB4P2-P AGB4P1-P AGB3P5-P AGB3P4-P AGB3P3-P AGB3P2-P AGB3P1-P AGB1P5-P AGB1P4-P AGB1P3-P AGB1P2-P AGB1P1-P

DGGE

PCR-DGGE

DGGE

UV transilluminator

Statistical analysis

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 20

DGGE prints of bacteria extracted from dry fishes

coming from three different farms (rainy season)

Chau Phu

An Phu

Chau Doc

E.Coli

CP1-R CP2-R CP3-R CP4-R CP5-R CP1-D CP2-D CP3-D CP4-D CP5-D AP1-R AP2-R AP3-R AP4-R AP5-R AP1-D AP2-D AP3-D AP4-D AP5-D CD1-R CD2-R CD3-R CD4-R CD5-R CD1 -D CD2 -D CD3 -D CD4 -D CD5 -D TC1-R TC2-R TC3-R TC4-R TC5-R TC1-D TC2-D TC3-D TC4-D TC5-D -12 -10 -8 -6 -4 -2 0 2 4 6 8 10

Dim en s ion 1 : 46.24% varia nces -8 -6 -4 -2 0 2 4 6 8 Dim e ns ion 2 : 26. 4 0 % v a ri a n c e s

CP: Chau Phu; CD: Chau Doc; TC: Tan Chau; AP: An Phu

Factorial analysis of DGGE fingerprint of bacteria from fishes of 4

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 22

Électronic

H

uman

Electronic nose to distinguish dates varieties

Electronic nose

Mass spectrometer

+ sensors

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 24

Distribution of Black Bousthammi, Deglet nour and their mix 50%

on first principal plan

Near Infra Red Spectrometry

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 26

Near Infra Red Spectrometry: principle

Diatomic vibring Molecule

Infrared

light

(ν

_v

, Ev)

(ν, λ)

E = hν

The SPIR is the measurement of the absorption (Wave length and intensity) of

the near infra-red light by a sample. The principle of the technic is based on the

vibrational properties of the molecules and their interactions with the light.

O

N

C H

N

N

N

H

C

O

C H

Comparaison to spectral data base

Infrared Light

Energy absorbance by Chemical Links

Cafein

Qualitative Analysis

Quantitative Analysis

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 28

Characterization of coffee mixtures (roasted and grounded)

R o b u s t a

A r a b i c a C P1 (63.86% )

C P 2 (29.85% )

Distribution of coffee arabica and

robusta on first principal plan

decaffeinated

Aromatized

with vanilla

defatted

Comparison of specific coffees to

standard

Linear discriminant analysis of three origins of

cocoa beans

Côte d'Ivoire

Trinidad

Venezuela

Classification rate 100%

Mathieu WEIL – V1 Kuala Lumpur 2nd of april 2008 30

Many

Many

Thanks

Thanks

for

for

your

your

attention !

attention !

www.cirad.fr/ur/qualite_aliments

www.cirad.fr

www.alcuefood.org