• Aucun résultat trouvé

Macrophage migration inhibitory factor regulates TLR4 expression and modulates TCR/CD3-mediated activation in CD4+ T lymphocytes

N/A
N/A
Info
Télécharger
Protected

Academic year: 2022

Partager "Macrophage migration inhibitory factor regulates TLR4 expression and modulates TCR/CD3-mediated activation in CD4+ T lymphocytes"

Copied!
4
0
0

Chargement.... (Voir le texte intégral maintenant)

Texte intégral

(1)Article. Macrophage migration inhibitory factor regulates TLR4 expression and modulates TCR/CD3-mediated activation in CD4+ T lymphocytes ALIBASHE AHMED, Mohamed, et al.. Abstract Toll-like receptor 4 (TLR4) is involved in CD4+ T lymphocyte-mediated pathologies. Here, we demonstrate that CD4+ T lymphocytes express functional TLR4 that contributes to their activation, proliferation and cytokine secretion. In addition, we demonstrate that TLR4-induced responses are mediated by macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine. We also demonstrate that MIF regulates suboptimal TCR/CD3-mediated activation of T lymphocytes. On one hand, MIF prevents excessive TCR/CD3-mediated activation of CD4+ T lymphocytes under suboptimal stimulation conditions and, on the other hand, MIF enables activated CD4+ T lymphocytes to sense their microenvironment and adapt their effector response through TLR4. Therefore, MIF appears to be a major regulator of the activation of CD4+ T lymphocytes and the intensity of their effector response. TLR4-mediated activation is thus an important process for T cell-mediated immunity.. Reference ALIBASHE AHMED, Mohamed, et al. Macrophage migration inhibitory factor regulates TLR4 expression and modulates TCR/CD3-mediated activation in CD4+ T lymphocytes. Scientific Reports, 2019, vol. 9, no. 1, p. 9380. PMID : 31253838 DOI : 10.1038/s41598-019-45260-6. Available at: http://archive-ouverte.unige.ch/unige:121103 Disclaimer: layout of this document may differ from the published version..

(2) Macrophage migration inhibitory factor regulates TLR4 expression and modulates TCR/CD3-mediated activation in CD4+ T lymphocytes Mohamed Alibashe-Ahmed, Thierry Roger, Veronique Serre-Beinier, Ekaterine Berishvili, Walter Reith, Domenico Bosco, and Thierry Berney. LPS ctrl 0. 0.12. 0.25. 0.5. 1. aCD3[µg/ml] Figure S1: LPS increases the number of IFN-producing T lymphocytes a) Representative images of IFN-ELISPOT of CD3+ T lymphocytes stimulated with LPS b) Qualitative measurement of IFN production represented by IFN-ELISPOT mean spot size in presence (red) or absence (black) of LPS Data are means of five experiments (b). Figure S2: LPS directly interacts with CD4+ T lymphocytes. Representative image of purified CD4+ T lymphocytes stained with FITC-labelled-LPS (blue) and without (red)..

(3) REAGENT or RESOURCE Antibodies low endotoxin azide free (LEAF) purified antimouse CD3ε antibody (145-2C11) low endotoxin azide free (LEAF) purified antimouse CD3ε antibody (17A2) Pure anti-CD28 (clone 37.51) Mouse Pan T Cell Isolation Kit II Mouse CD4+ T Cell Isolation Kit FITC anti-mouse CD25 Antibody (3C7) PE/Cy7 anti-mouse CD69 Antibody (H1.2F3) PE anti-mouse CD69 Antibody (H1.2F3) PE/Cy7 anti-mouse CD8a Antibody (53-6.7) PE anti-mouse CD8a Antibody (53-6.7) APC/Cy7 anti-mouse CD8a Antibody (53-6.7) APC anti-mouse CD8a Antibody (53-6.7) PerCP anti-mouse CD4 Antibody (RM4-5) PE/Dazzle™ 594 anti-mouse CD4 Antibody (GK1.5) PE anti-mouse CD3 Antibody (17A2) FITC anti-mouse IFN-γ Antibody (XMG1.2) PE anti-mouse IL-2 (JES6-5H4) FITC Rat IgG1 κ Isotype Ctrl Antibody (RTK2071) PE Rat IgG2b κ Isotype Ctrl Antibody (RTK4530) FITC Rat IgG2b κ Isotype Ctrl Antibody (RTK4530) PE Rat IgG2a κ Isotype Ctrl Antibody (RTK2758) PE Armenian Hamster IgG Isotype Ctrl Antibody (HTK888) PE/Cy7 Rat IgG1 κ Isotype Ctrl Antibody (RTK2071) Zombie Red™ Fixable Viability Kit ( Bacterial and Virus Strains ultrapure LPS from E. coli O111:B4 ultrapure LPS from P.gingivalis ultrapure LPS from S. minnesota R595 FITC-conjugated Lipopolysaccharides from Escherichia coli O111:B4 Chemicals, Peptides, and Recombinant Proteins CLI-095 CFSE recombinant mouse MIF ISO-1 Critical Commercial Assays Cell Activation Cocktail Intracellular Fixation & Permeabilization Buffer Set. SOURCE. IDENTIFIER. Biolegend. 100314. Biolegend. 100208. Milteyni Biotec Miltenyi Biotec Miltenyi Biotec. 130-093-182 130-095-130 130-104-454. Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend Biolegend. 101908 104512 104508 100708 100722 100714 100711 100537 10455 100205 505806 503807 400405 400607 400634 400508 400907. Biolegend Biolegend. 400416 423110. Invivogen Invivogen Invivogen Sigma-Aldrich. Tlrl-3pelps Tlrl-ppglps Tlrl-smlps F3665-1MG. Invivogen Life Technologies Biolegend Merck Millipore. Tlrl-cli95 C34554. Biolegend eBioscience. 423303 88-8824-00. 475837.

(4) Mouse IL-2 Ready-SET-Go! kits Mouse IFN Ready-SET-Go! kits Mouse TNF Ready-SET-Go! kits Mouse IFN from ELISPOT Ready-SET-Go Mouse MIF ELISA kit High Capacity cDNA Reverse transcription kit PeqGold Trifast TaqMan Fast Advance Master Mix Experimental Models: Organisms/Strains Mouse NOD/BDC2.5 NOD.CgTg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ (NOD/BDC2.5) Mouse TLR4-/- B6.B10ScN-Tlr4lps-del/JthJ Mouse TLR4+/+ C57BL/6J Mouse MIF-/-. Mouse MIF+/+. Oligonucleotides Primer mouse Rplp0 (Mm99999223_gH) Primer mouse Tlr4 (Mm00445273_m1) Software and Algorithms GraphPad Prism 7 FlowJo V_10 Supplementary Table S1: list of materials.. Thermofischer Thermofischer Thermofischer Thermofischer Biomatik ThermoFischer Scientific PeqLab ThermoFischer Scientific. 88-7024-22 88-7314-22 88-7324-22 88-7384-21 EKU05762 4368814. Jackson Laboratory. 004460. Jackson Laboratory Jackson Laboratory John David (Harvard Medical School, Boston, MA). 007227 000664. 30-2010 4444556. John David (Harvard Medical School, Boston, MA) ThermoFischer Scientific ThermoFischer Scientific GraphPad Software FlowJo, LLC. 4331182 4331182.

(5)

Références

Télécharger maintenant ( PDF - 4 Page - 2.31 MB )

Documents relatifs

Etude de l’hybridation inter-spécifique entre espèces du genre Coffea en Nouvelle-Calédonie : distribution des niches favorables et structuration de la diversité

Les niches et la distribution des espèces peuvent être reliées selon quatre théories écologiques (Pulliam, 2000) : (1) la niche « Grinellian » qui considère

Section : Sciences expérimentales

Donner l’équation de la droite puis Calculer la distance de freinage lorsque la voiture roule à 100km/h. 4°) La vitesse de la voiture est de 140 km/h, lorsque le

Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex

Abstract In an analysis of a microsatellite locus (OLADRB1) linked to the MHC DRB1 gene of Iberian and Alpine ibex (Capra pyrenaica and Capra ibex), we detected strong

Response of Net Ecosystem Productivity of Three Boreal Forest Stands to Drought

Interannual and seasonal variation in ecosystem respiration (R) (left panel) and gross ecosystem productivity (GEP) (right panel) observed at the three sites in 2000 (dashed thin

Transformation of five grape rootstocks with plant virus genes and a virE2 gene from Agrobacterium tumefaciens

Agrobac- terium-mediated genetic transformation of grapevine somatic embryos and regeneration of transgenic plants expressing the coat protein of grapevine chrome mosaic

Non-signature based virus detection : Towards establishing a unknown virus detection technique using SOM

Among the 790 virus-infected test files, there were 30 Win9x encrypted and 15 polymorphic parasitic viruses. These represent about 15% of the tested Win9x virus- infected files. In

New anti-Paenibacillus larvae substances purified from propolis

Samples in DMSO (total amount applied in 5 μL): B1, benzyl ferulate; B2, pentenyl ferulate; B3, caffeate mixture average; B4, 9- oxo-10(E),12(Z)-octadecanoic acid; B5, pinocembrin;