InnOscent chromatographic system: An innovative device to revisit aroma analysis and recombination perspectives

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InnOscent chromatographic system: An innovative

device to revisit aroma analysis and recombination

perspectives

Angélique Villière, Sarah Le Roy, Catherine Fillonneau, Carole Prost

To cite this version:

Angélique Villière, Sarah Le Roy, Catherine Fillonneau, Carole Prost. InnOscent chromatographic system: An innovative device to revisit aroma analysis and recombination perspectives. 15th Weurman Flavour Research Symposium - Flavour Science, Sep 2017, Graz, Austria. �hal-01945107�

InnOscent chromatographic system:

An innovative device to revisit aroma analysis and recombination perspectives

ONIRIS - College of Veterinary Medicine and Food Science, Flavor group

GEPEA - UMR CNRS 6144, Nantes, France

Angélique VILLIÈRE, Sarah LE ROY,

Catherine FILLONNEAU, Carole PROST

GC-MS-O is very helpful to identify odorants in aromas. However, there is a gap between this individual characterization and

the effective contribution of compounds to aromas, notably due various interactions. Thus, many aroma investigations turn to

reconstituted models and recombination experiments with chemicals, a valuable but time-consuming approach.

InnOscent device was designed to realize both conventional GC-MS-O analysis and recombination-omission experiments.

These capabilities are illustrated hereafter through a wine aroma analysis and contribution of odorants to global aroma was

evaluated.

angelique.villiere@oniris-nantes.fr

 InnOscent device permits to achieve both conventional GC-O analysis and recombination or omission experiments,

enabling a comprehensive analysis of aromas.

 It offers a valuable alternative to current approaches notably those using chemicals and

overcome limitations related to identification, quantitation, and commercial availability of

compounds.

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10

15

20

25

Number

of

de

tections

(N

)

Retention time (min)

33 odorant

zones were

significantly

detected

Significance level

Compounds perceived by at least 4 judges N ≥ 4

Compounds perceived by at least 6 judges N ≥ 6

Total extract

Compounds perceived by at least 3 judges N ≥ 3

Compounds perceived by at least 8 judges N ≥ 8

Dual facilities are achieved by a wise

combination of splitter and Deans

switch microfluidics transfer modules.

GC-MS-dual Olfactometric port analysis

Recovering selective fractions with GC-MS-FID control

Splitting

and

switching

disposal

Mass

spectrometer

Multiple

and flexible

detection

and

recovery

capabilities

Dual human

nose detection

FID

Gastight

collector

InnOscent chromatographic device

(patent pending)

Representative wine aroma extraction

Car/PDMS

SPME fiber

T = 34°C

Extraction time = 10 min.

Extracts with only

compounds

detected by

Reference = total extract

contains all compounds

GC-InnOscent enables a comprehensive aroma analysis.

10

8,2

a

7,7

a

7,4

a

7,2

a

2,2

b

Similarity of extracts vs reference

0

Extracts designed with compounds significantly

detected by GC-O are perceived similarly to the

hidden reference.

The Mixture of compounds perceived by at least 6

judges is also perceived similarly to the hidden

reference.

Sensory evaluation of recovered extracts

8 trained judges panel

8 judges trained to

aroma recognition

However, the mixture of the only major odorants

does not recreate the odor of the original wine.

 Freeing from chemicals, InnOscent makes it possible to evaluate the contribution of any

compound to an overall aroma.

N = 8

N ≥ 6

N ≥ 4

N ≥ 3

Hidden reference

_{The similarity of the hidden reference vs reference,}

is scored at 8,2/10 instead of 10, due to natural

reluctance of judges to use ends scale.