Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes

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Reference

Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes

LEBRETON, Fanny, et al.

Abstract

Maintaining long-term euglycemia after intraportal islet transplantation is hampered by the considerable islet loss in the peri-transplant period attributed to inflammation, ischemia and poor angiogenesis. Here, we show that viable and functional islet organoids can be successfully generated from dissociated islet cells (ICs) and human amniotic epithelial cells (hAECs). Incorporation of hAECs into islet organoids markedly enhances engraftment, viability and graft function in a mouse type 1 diabetes model. Our results demonstrate that the integration of hAECs into islet cell organoids has great potential in the development of cell-based therapies for type 1 diabetes. Engineering of functional mini-organs using this strategy will allow the exploration of more favorable implantation sites, and can be expanded to unlimited (stem-cell-derived or xenogeneic) sources of insulin-producing cells.

LEBRETON, Fanny, et al . Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes. Nature Communications , 2019, vol. 10, no. 1, p. 4491

PMID : 31582751

DOI : 10.1038/s41467-019-12472-3

Available at:

http://archive-ouverte.unige.ch/unige:134290

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Author Correction: Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes

Fanny Lebreton , Vanessa Lavallard , Kevin Bellofatto, Romain Bonnet, Charles H. Wassmer, Lisa Perez, Vakhtang Kalandadze, Antonia Follenzi , Michel Boulvain, Julie Kerr-Conte, David J. Goodman,

Domenico Bosco , Thierry Berney & Ekaterine Berishvili

Correction to:Nature Communicationshttps://doi.org/10.1038/s41467-019-12472-3, published online 3 October 2019.

In the original version of this Article the ethical registration number permitting collection of amniotic membranes in the Human samples subsection of the“Methods”section was mis-cited as 2017-00101. The correct number refers to the pre-Basec study PB_2017- 00101, which is not listed in the Register of Research Projects in Switzerland (RAPS). The old reference number of this study is 14-273 and can be found in a list of protocols submitted before 2015 (https://www.ge.ch/document/liste-protocoles-soumis-2015).

This has been corrected in both the PDF and HTML versions of the Article.

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© The Author(s) 2020

https://doi.org/10.1038/s41467-020-19127-8 OPEN

NATURE COMMUNICATIONS| (2020) 11:5159 | https://doi.org/10.1038/s41467-020-19127-8 | www.nature.com/naturecommunications 1

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