Field Phenotyping for drought tolerance

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Field Phenotyping for drought

tolerance

A. Audebert

2

nd

_{Global Rice Phenotyping Network Worshop}

IRRI 22-24 November 2012

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Field phenotyping for drought tolerance

• Based on Infra-red thermography.

– Canopy temperature give an indication of the leaf surface cooling capacity by transpiration along environmental conditions

– Could be use as a trait for phenotyping

• (indirect evaluation of drought)

– This trait depending of

• Environmental conditions

– Air temperature

– Wind speed

– Solar radiation

– Evaporative demand (VPD)

• Sol water conditions

– Humidity / available

• Plant characteristics

– Surface of canopy

– Plant Architecture

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Pictures

Information Bloc Identity Line Identity Control temperature Aluminum paper Soil humidity

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Difficulties to solve

• Environmental conditions highly variable

• Quick plant reaction

– Wind – Radiation

• Low equipment (camera and 1 technician)

• Impossible to have one unique picture for the whole experiment

– Helicopter – Drone

• Optimum 3-4 lines per image

– 400 lines -> 100 -120 pictures – 1 image per minute

• Time for measurement

– About 2-3 hours

– Environmental stability

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Using CWSI

• Quantifying the water stress with correlation between canopy

temperature and evaporative demand

– VPD

– CWSI (Crop water stress index)

• Need simultaneously measurement of the evaporative demand

– Weather station

– Psychrometer measurement

• Humid and dry temperature

min

)

(

max

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(

min

)

(

)

(

Ta

Ts

Ta

Ts

Ta

Ts

Ta

Ts

CWSI

−

=

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Results

Min. C Max. C Avg. C

Rectangle 1 30,5 32,8 31,1 Rectangle 2 30,4 32,3 31,0 Rectangle 3 29,1 32,5 30,2 Rectangle 4 28,8 31,4 30,0 Line 5 29,8 31,9 30,7 Rectangle 6 29,7 31,9 30,5 Line 19 : 31.05 °C Line 20 : 30.10 °C Line 21 : 30.60 °C

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Results

Control Stress C anopy t em per at ur e (°C ) 20 22 24 26 28 30 32 34 36 38 Stress 29.305 °C +/- 1.46 Control 24.771 °C +/- 1.62 2009-2010 Soil humidity (%) (10-20) 10 12 14 16 18 20 22 C anopy t em per at ur e (°C ) 20 25 30 35 40 Control Stress Y = -0.42 X + 37.78 r ² = 0.43; *** Soil Humidity (%) (0-10 cm) 6 8 10 12 14 16 18 20 22 24 26 C anopy t em per at ur e (°C ) 27 28 29 30 31 32 33 34 35 Esperanza Fedearroz50 IRAT 216 Saquarema

Direct relation between

Soil humidity and

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Results

Canopy temperature (°C) 28 30 32 34 36 38 Y iel d (g) 0 200 400 600 800 1000 canopy temperature (°C) 30 31 32 33 34 35 36 37 Y iel d (g) 0 100 200 300 400 Esperanza Fedearroz IRAT216 Saquarema

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Phenotyping experiment

• Dry season

• Field experiment

– Villavicencio station “Santa Rosa “(Colombia) • 240 varieties tested with 2 reps

• Lines 3 meters

• Stress period 3 weeks (29/01-19/02/2010)

• Vegetative stage

• Design

– Alpha lattice 8 sub-Blocs with 2 replications – Complete randomization

– 5 control varieties repeated

• Measurements

– IR thermography once a week during the stress – Soil humidity

– Microclimatic data

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Delta -30 -29 -28 -27 -26 -25 N um ber of genot ypes 0 5 10 15 20 25 30 SD = 0.662

Temperature and WUE Diversity

Crop Water Stress Index

0,4 0,5 0,6 0,7 0,8 0,9 1,0 N um ber of genot ypes 0 5 10 15 20 25 30 SD = 0.111

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But

∆C13 % -29,5 -29,0 -28,5 -28,0 -27,5 -27,0 -26,5 -26,0 -25,5 C W S I -0,4 -0,2 0,0 0,2 0,4 0,6 0,8 1,0 ∆C13 % -29,5 -29,0 -28,5 -28,0 -27,5 -27,0 -26,5 -26,0 -25,5 C anopy t em per at ur e (°C ) 26 28 30 32 34 36 38 40

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Conclusions

• The phenotyping was done (vegetative stage)

• The use of the CWSI allows to compare cultivars between them during the phenotyping time

• Good diversity is observed for transpiration and CID • Association study will be done further with SNPs • Phenotyping at reproductive stage

– With adapted sowing date

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