Combined mRNA and microRNA profiling reveals that miR-148a and miR-20b control human mesenchymal stem cell phenotype via EPAS1

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Submitted on 7 Feb 2012

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Combined mRNA and microRNA profiling reveals that miR-148a and miR-20b control human mesenchymal

stem cell phenotype via EPAS1

Geneviève Pietu, Jérôme Alexandre Denis, Geneviève Pietu, Christelle Rochon-Beaucourt

To cite this version:

Geneviève Pietu, Jérôme Alexandre Denis, Geneviève Pietu, Christelle Rochon-Beaucourt. Combined mRNA and microRNA profiling reveals that miR-148a and miR-20b control human mesenchymal stem cell phenotype via EPAS1. Physiological Genomics, American Physiological Society, 2011, 43 (2), pp.77. �10.1152/physiolgenomics.00077.2010�. �hal-00563934�

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A

3' GAUGGACGUGAUACUCGUGAAAC

||||||

5' ...UUUUUCUGAGAUGCUCACUUUAU...

EPAS1 miR20b

5' ...UGCUAGCCCUUCAAG--UGCACUGA...

EPAS1

miR148a 3' UGUUUCAAGACAUCACGUGACU

||| |||||||

Figure 3

D C

B

PLAUR

0 20 40 60 80

MSC+CTRL MSC+miR148a MSC+miR20b MSC+miR429

mRNA expression level

PLAU

0 10 20 30 40

GBE1

0 20 40 60 80

HIF1A

0 5 10 15

NDRG1

-5 5 15 25 35

LOX

0 500 1000 1500

DUSP1

0 10 20 30

PLOD2

0 50 100 150

D