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Early life stressful events impaired enteric antimicrobial activity and triggered commensal E. coli overgrowth responsible for visceral hypersensitivity in adult mice

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Academic year: 2021

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HAL Id: hal-01605362

https://hal.archives-ouvertes.fr/hal-01605362

Submitted on 6 Jun 2020

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Early life stressful events impaired enteric antimicrobial

activity and triggered commensal E. coli overgrowth

responsible for visceral hypersensitivity in adult mice

Vassilia Theodorou, Sandrine Ménard, Corinne Lencina, Mathilde Lévêque,

Maïwenn Olier, Viorica Braniste, Manon Naturel, Soraya Sekkal, Cherryl

Harkat, Eric Houdeau

To cite this version:

Vassilia Theodorou, Sandrine Ménard, Corinne Lencina, Mathilde Lévêque, Maïwenn Olier, et al.. Early life stressful events impaired enteric antimicrobial activity and triggered commensal E. coli overgrowth responsible for visceral hypersensitivity in adult mice. 49. Annual Meeting of Society of Toxicology and ToxExpo, Mar 2013, Salt Lake City, United States. �hal-01605362�

(2)

EARLY LIFE STRESSFUL EVENTS IMPAIRED ENTERIC ANTIMICROBIAL ACTIVITY AND TRIGGERED COMMENSAL E. COLI OVERGROWTH RESPONSIBLE FOR VISCERAL

HYPERSENSITIVITY IN ADULT MICE

A. Riba1, M. Olier1, S. Lacroix-Lamandé4, C. Lencina1, V. Bacquié1, C. Harkat1, M. Gillet1, M. Baron1, C. Sommer2, V. Mallet1, C. Salvador-Cartier3, F. Laurent4, V. Théodorou1, S. Ménard1. INRA Toxalim, Research Centre in Food Toxicology, 1NeuroGastroenterology & Nutrition, 2EZOP,

Experimental Zootechnique Platform, 3Intestinal Development, Xenobiotics & ImmunoToxicolocy, Toulouse, France, 4Equipe Apicomplexes et Immunité Mucosale (AIM), UMR 1282

INRA/Université-Infectiologie et Santé Publique (ISP), Centre INRA Val de Loire, Nouzilly, FRANCE

200/200 words

Early life stressful events induce long lasting alterations of intestinal homeostasis associated with susceptibility to develop gastrointestinal disorders at adulthood. Neonatal period is characterized by immature intestinal mucosa. Among others, Paneth cells appear only 2 weeks after birth. Our aim was to analyze the consequences of maternal separation (MS) in mice on enteric antimicrobial activity and its consequences on intestinal microbiota, systemic immune response toward microbiota and visceral sensitivity. In 50-days old mice, MS induced a decrease of enteric antimicrobial activity associated with intestinal E. coli overgrowth and an increase of anti-E. coli IgG and IgA in plasma. Furthermore, MS increased IFNγ and TNFα in ileum and induced visceral hypersensitivity in response to colorectal distension. In order to decipher whether or not those alterations were a consequence of E. coli overgrowth, adult mice were force fed daily with 109 commensal E. coli for 15 days. E. coli gavage reproduced intestinal E. coli overgrowth as well as anti-E. coli IgG and IgA increase and visceral hypersensitivity without modification of enteric antimicrobial defense. Altogether our results highlighted that early life stressful events impair the development of antimicrobial defenses and promote commensal bacterial overgrowth leading to abnormal response toward microbiota and visceral hypersensitivity.

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