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Creation of a white inflorescence colour cultivar of Alpinia purpurata through the combination of intergeneric hybridization and mutagenesis : [poster]

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CONCLUSION

Intergeneric hybridization between A.  purpurata and  E.  elatior was a  way to  increase fertility in Alpinia purpurata. Induced mutagenesis was the way bringing more variation concerning the colour type.

PERSPECTIVES

Other  new  colours,  shapes  and  sizes  are  seeking  out.  A  team  of  growers  and  breeders is doing this work of improving Alpinia purpurata.

INTRODUCTION

Apinia purpurata an ornamental species of the Zingiberaceae is the most  important commercial cut flower of the West Indies for export to Europe and  North America. Nevertheless, only two colours are commercially available in  this species, the red A. purpurata (no cultivar name) and its pink cv. Eileen  McDonald. Therefore, research is in progress using tools for improving and  obtaining new colour type cultivars.

Introduction traits from other genera Etlingera elatior (Jack) R.M. Smith increase  the range of variation (Luc‐Cayol and Fereol 1997). 

Mutagenesis is also an important tool, particularly for morphological variations  and  colour  type,  in  different  ornamental  plants.  Fereol,  Louis  et  al.  (1996)  reported that this technique has potential for increasing the genetic variation in  Alpinia purpurata. 

The objective of this investigation was to explore the possibility of introducing  new  genes  for  inflorescence  colour  and  shape  through  the  combination  of  intergeneric hybridization and mutagenesis.

Creation of a white inflorescence colour cultivar 

of Alpinia purpurata through the combination of 

intergeneric hybridization and mutagenesis

INDUCED MUTAGENESIS

L. Fereol1,2,3, F. Luc‐Cayol1, L. Luce4& M. Guitteaud1

1. Antilles Biotechnologies, S/C Miguel Guitteaud, 55 bld Amilcar Cabral, Lotissement de Cluny, 97200 Fort de France, Martinique (W.I.); 2. CIRAD, Station de Petit  Morne, 97232 Le Lamentin, Martinique (W.I.); 3. INRA, BP 515, 97165 Pointe à Pitre, Guadeloupe (W.I.); 4. Centre Hospitalier Universitaire, Service Oncologie, 97110  Pointe à Pitre, Guadeloupe (W.I.)

http://antilles‐biotech.com;   E‐mail: info@antilles‐biotech.com

INTERGENERIC HYBRIDIZATION

About  20%  of  the  pollinations  yielded  viable  hybrid  seeds.  Hybrid  seedlings differed from A. purpurata by increasing production of flowers. A  total  of  88  hybrids  observed  revealed  they  were  fertile  and  produce  more  flowers  than  A.  purpurata,  and  fruits  were  formed  from  these  flowers  without manual pollination, traits similar to the staminate parent.

Comparatively,  in  a  series of  reciprocal intra  –specific crosses  in  A.  purpurata over  3  years,  using the  red‐bracted and  the  pink‐braced selections, the crosses yielded only 10% fruit set. 

. Fereol, L., S. Louis, et al. (1996). ʺEffects of gamma radiation on in vitro plantlets of Alpinia

purpurata.ʺ Journal of Horticultural Science 71(2): 243‐247.

. Luc‐Cayol, F. and L. Fereol (1997). ʺX Alpingera martinica (Zingiberaceae): An intergeneric Hybrid between Alpinia purpurata and Etlingera elatior.ʺ HortScience 32(5): 914‐915.

References :

A  population  of  300  seeds  from  this  back‐cross  was  treated  with  gamma  radiation at a dose of 30 Gy.

After  treatment,  these  seeds  were  planted  in  30x40‐cm  trays  in  the  green  house,  and  they  were  transplanted  to  the  field  3  months  later  for  evaluation  and comparison to the parents.

Plantlets morphological characteristics were observed. They were grown to 14‐ month old flowering plants to observe their floral characteristics.

Irradiated plants showed a number of morphological abnormalities. These are  of  interest  in  ornamental  horticulture.  Abnormal  plantlets  were  called  “off‐ types”. Selection based on individual stalks was continued for five generations  until  the  “off‐types” were  stable.  Variations  became  stable  from  the  C4  generation.

OBTENTIONTION OF A WHITE GINGER 

« MADIKERA WHITE N°2 »

X

♀ ♂ Alpinia purpurata Etlingera elatior Alpingera martinica Seeds gamma irradiated at 30 Gy dose

Plantletss from irradiated seeds

From this investigation, it was found that one plant possessed the characteristics of white

inflorescence. The characteristics of such new

cultivar can be summarized as follows:

a) Vigorous vegetative morphology similar to Alpinia purpurata,

b) Attractive spike-like inflorescences having obloid morphology like A. purpurata, bearing well overlapping bracts of white colour,

c) Bracts acute tip shape but large at the base,

d) Inflorescence size up to 25 cm,

e) Vase life of these inflorescences up to 20 days, f) Number of inflorescence /plant/year about 60.

It has been called, white ginger “Madikera white N°2”.

White gingerʺ Madikera white N°2 "

Field grown plants  of  a  pink‐bracted selection  (“Eilen McDonald”)  of  A.  purpurata were  cross‐pollinated  with  a  rose‐pink  selection  of  E.  elatior under field

conditions.  Both  species  are  4x=48.  A.  purpurata was  the  pistillate parent.  Intergeneric hybrids  between  both  selections  were  achieved  with  relative ease.

BACK‐CROSS

X

The  intergeneric

hybrids  plants  were  back‐crossed  with  A. 

purpurata pink‐ bracted selection  (“Eilen McDonald”)  under  field  conditions.  These  intergenric

hybrids  were  pistillate

parent.  Pollinated 

flowers  were  tagged  and  seeds  were  collected  3  months  later. About  30%  of  the  pollinations  yielded  viable  hybrid  seeds.

Alpingera martinica

Alpinia purpurata

Population of seeds coming from the back‐cross

IAEA‐CN‐167‐211P

Well over lap bracts

Spike like inflorescence Having obloid morphology

Numerous inflorescences Vigorous vegetative

Références

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