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Antimicrobial resistan ce of shiga toxin gene
(stx)-positive and initimin gene (eae)-positive
Escherichia coli isolates from wastewaters of an urban
wastewater treatment plant (WWTP) collecting
slaughterhouse effluents
Delphine Bibbal, Véronique Dupouy-Guiraute, Alpha Amadou Diallo,
Monique Kérourédan, Nathalie Arpaillange, Pierre-Louis Toutain, Eric
Oswald, Alain Bousquet-mélou, Hubert Brugère
To cite this version:
Delphine Bibbal, Véronique Dupouy-Guiraute, Alpha Amadou Diallo, Monique Kérourédan, Nathalie
Arpaillange, et al.. Antimicrobial resistan ce of shiga toxin gene (stx)-positive and initimin gene
(eae)-positive Escherichia coli isolates from wastewaters of an urban wastewater treatment plant (WWTP)
collecting slaughterhouse effluents. 3rd ASM Conference on Antimicrobial Resistance in Zoonotic
Bacteria and Foodborne Pathogrens in Animals, Humans, and the Environmen, American Society for
Microbiology. USA., Jun 2012, Aix-en-Provence, France. �hal-02746917�
ASM Conferences
146
ESBL carriage rate was even higher (33%). A difference in the overall pattern of virulence associated genes with respect to their ESBL phenotype could not be observed. Thus, the presence of strains with high numbers (> 20) of genes, resembling a virulence pattern which is also typical for human extraintestinal patho-genic E. coli (ExPEC), was not linked with the ESBL status but rather with the phylogenetic background of a strain. Multilocus sequence types (STs) determined for the rodent ESBL-E. coli, including ST410 and ST90 have previ-ously also been found in ESBL-E. coli from humans, pets, and livestock Initial comparative analysis of rodent ESBL isolates with fifty human clinical strains, sampled in the same time and local, raise the question as to whether urban rats might act as a reservoir for ESBL-E. coli or constitute a common source of envi-ronmental pollution with multiresistant strains which in turn would represent a serious issue of public health.
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ANTIMIcRObIAL RESISTANcE Of SHIGA TOxIN GENE (stx)-POSITIVE ANd INITIMIN GENE (eae)-POSITIVE escherichia coli ISOLATES fROM WASTEWATERS Of AN uRbAN WASTEWATER TREATMENT PLANT (WWTP) cOLLEcTING SLAuGHTERHOuSE EffLuENTS
Bibbal Delphine 1, Dupouy Véronique 2, Diallo
Alpha Amadou1,3, Kérourédan Monique1,
Ar-paillange Nathalie 2, Toutain Pierre-Louis2,
Oswald Eric 4, Bousquet-Mélou Alain 2 and
Brugère Hubert 1
1 UMR1043, INSERM, INRA USC1360,
INP-ENVT, Université de Toulouse, Toulouse, France
2 UMR1331 Toxalim, INRA, INP-ENVT,
Uni-versité de Toulouse, Toulouse, France
3 ISRA / LNERV, Laboratoire National de
l’Ele-vage et de Recherches Vétérinaires, Dakar-Hann, Sénégal
4 INSERM UMR1043, INRA USC1360, CHU
de Toulouse, Hôpital Purpan, Toulouse, France
Introduction & Objectives: Enterohemor-rhagic Escherichia coli (EHEC) strains are responsible for severe clinical symptoms in hu-mans. Typical EHEC strains carry stx and eae genes. The objective of this study was to de-termine the antimicrobial resistance of stx- and
eae-positive E. coli strains, previously isolated from wastewaters of an urban WWTP, col-lecting slaughterhouse effluents. Material & Methods: The antimicrobial resistance of 143
stx- and/or eae-positive isolates was investi-gated by disk diffusion method. These isolates were obtained from the input effluents from the slaughterhouse (55), and the city (41), and from the output treated effluent (47 isolates, among which four stx2- and eae-positive E.
coli O157:H7 isolates). Results: Concerning the animal isolates, 71% were resistant, mainly to tetracycline. Concerning the human isolates, 24% were resistant, mainly to ampicillin and tetracycline. However, we have detected an ESBL-producing E. coli in the input effluent from the city. This isolate carried the blaCTX-M-14 and was positive for the eae-β1 gene. Concern-ing the output effluent, 28% of the isolates were resistant. The four stx2- and eae-positive
E. coli O157:H7 isolates were susceptible to all tested antibiotics. Conclusions: Our results suggest that the profile of antimicrobial resist-ance of stx- and eae-positive E. coli seemed to be different between human and animal origin. In addition, the isolates showing high virulence for humans were susceptible to all tested anti-biotics.
