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Purification of a 47-kDa calmodulin-binding polypeptide as an actin-binding protein from Neurospora crassa

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Purification of a 47-kDa calmodulin-binding polypeptide as an actin-binding protein from Neurospora crassa

CAPELLI, Nicolas, et al.

Abstract

We have enriched a 47-kDa polypeptide (p47) from Neurospora crassa on the basis of its affinity to calmodulin. The p47 was purified to homogeneity by chromatography on a Mono S cation exchange column and evidence is presented that the polypeptide co-sediments specifically with F-actin. The intracellular distribution of p47 and actin was also examined using indirect double immunofluorescence staining of cells at different stages of development.

Our results suggest that by altering the conformation binding site of actin to p47, calmodulin could play a regulatory role in the polarized hyphal growth of N. crassa.

CAPELLI, Nicolas, et al . Purification of a 47-kDa calmodulin-binding polypeptide as an

actin-binding protein from Neurospora crassa . FEMS Microbiology Letters , 1997, vol. 147, no. 2, p. 215-220

DOI : 10.1111/j.1574-6968.1997.tb10244.x

Available at:

http://archive-ouverte.unige.ch/unige:124814

Disclaimer: layout of this document may differ from the published version.

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Erratum

Erratum to ``Puri¢cation of a 47-kDa calmodulin-binding polypeptide as an actin-binding protein from Neurospora crassa''

[FEMS Microbiol.Lett.147 (1997) 215^220]

1

N.Capelli

a

, F.Barja

c

, D.van Tuinen

b

, J.Monnat

c

, G.Turian

c

, R.Ortega Perez

c;

*

aLaboratory of Biochemistry and Plant Physiology, 3 Place de l'Universiteè, University of Geneva, CH-1211 Geneva 4, Switzerland

bLaboratory of Phytoparasitology, I.N.R.A.-SGAP, BV 1540, 21034 Dijon Cedex, France

cLaboratory of General Microbiology, Sciences III, University of Geneva, CH-1211 Geneva 4, Switzerland Received 27 February 1997

The legend from Fig.2 (p.218) was not printed correctly.The ¢gure and legend should have appeared as printed below.

The Publisher apologizes for any inconvenience this may have caused.

0378-1097 / 97 / $17.00 ß 1997 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

PII S 0 3 7 8 - 1 0 9 7 ( 9 7 ) 0 0 0 9 2 - X

* Corresponding author.Tel.: +41 (22) 702 6762;

fax: +41 (22) 781 1747; e-mail: ortega@sc2a.unige.ch

1PII of original article S0378-1097(96)00527-7

FEMS Microbiology Letters 150 (1997) 173

Downloaded from https://academic.oup.com/femsle/article-abstract/150/1/173/513031 by University de Geneve user on 23 October 2019

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Purification of a 47-kDa calmodulin-binding polypeptide as an actin-binding protein from Neurospora crassa

We report here the puri¢cation and localization of a 47-kDa peptide (p47) able to bind not only to actin but also to calmodulin (CaM), an intracellular cal- cium regulator that we

Erratum to "Purification of a 47-kDa calmodulin-binding polypeptide as an actin-binding protein from Neurospora crassa” : [FEMS Microbiol. Lett. 147 (1997) 215-220]

c Laboratory of General Microbiology, Sciences III, University of Geneva, CH-1211 Geneva 4, Switzerland Received 27 February 1997. The legend from Fig.2 (p.218) was not

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