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Double methylation of tRNA-U54 to 2′-O-methylthymidine (Tm) synergistically decreases immune response by Toll-like receptor 7

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Academic year: 2021

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Figure 1. RNA modifications in mammalian tRNA Lys 3 . Left part: native tRNA Lys 3 and its corresponding modifications (illustrated as gray circles).
Figure 2. Native tRNA Lys3 and its immunostimulatory activity. (A) Human PBMCs were stimulated with DOTAP encapsulated bacterial RNA, an un- un-modified Lys3 tRNA and native tRNA Lys 3 (see Supplementary Figure S1A) at three different concentrations (0.5,
Figure 3. Colicin D-based molecular surgery and preparation of Lys3-modivariants. (A) Treatment of native tRNA Lys 3 (gray line, modifications indicated as gray dots) with Colicin D generated two fragments with 38 nt length (see PAGE analysis)
Figure 5. Synthesis of Tm mv & permutated modivariants. (A) Reaction scheme describing the conversion of Tm to the corresponding bisphosphate by diphosphoryl chloride addition (B) Left part: TLC analysis of crude product (cp) showed the formation of 5
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