Abstract: Genetic Determinism o f the Fatty Acid Composition o f Palm Oil in Elaeis (<em>E. guineensis</em> Jacq. and <em>E. oleifera</em> (H.B.K) Cortés) by Gen..
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r i . ’’"."'! Plant and Animal Genome XXII ConferenceJanuary 10 -15, 2014
W550
Genetic Determinism of the Fatty Acid Composition of Palm Oil in Elaeis (£. guineensis Jacq. and E. oleífera (H.B.K) Cortés) by Genetic Mapping and Differential Expression Analysis of Candidate Genes
Date: Sunday, January 12, 2014 Time: 3:30 PM
Room: Towne
Carmenza Montoya, CENIPALMA, Bogotá D.C., Colombia Teresa Cuellar, CIRAD, Montpellier, France
Ricardo Lopes, EMBRAPA, Manaus, Brazil Marilyne Sum m o , CIRAD, Montpellier, France Daniela Bittencourt, EMBRAPA, Manaus, Brazil Céline Pinaglia, CIRAD, Montpellier, France Ronan Rival I a n , CIRAD, Montpellier, France Sandra Espeout-Fois, CIRAD, Montpellier, France Bertrand Pitollat, CIRAD, Montpellier, France Albert Flori, CIRAD, Montpellier, France Pierre Villeneuve, CIRAD, Montpellier, France Michel Pina , CIRAD, Montpellier, France Benoit Cochard, CIRAD, Montpellier, France David Cros, CIRAD, Montpellier, France Javier Herrero, NEIKER, Vitoria, Spain Alphonse Omore, INRAB, Pobè, Benin
Jorge Zambrano, Hacienda La Cabaña S.A., Bogota D.C., Colombia Indra Syaputra , SOCFINDO, Medan, Indonesia
WilmarH. Alarcon, Indu pal ma, Bogota D.C., Colombia Bruno Nouy, PalmElit SA, Montferrier/Lez, France Philippe Amblard, PalmElit SA, Montferrier/Lez, France
Thierry Leroy, CIRAD, Montpellier, France Enrique Ritter, NEIKER, Vitoria, Spain
Norbert Billotte, CIRAD, Montpellier, France
In this study of the International Oil Palm Genome Projects (OPGP) Consortium, an Elaeis interspecific pseudo-backcross of first generation (£. oleífera x E. guineensis) x E. guineensis allowed tracing segregating alleles from both Elaeis genomes. Nineteen quantitative trait loci (QTLs) associated to palm oil fatty acid proportions compared to 16 QTLs of same traits in an intra-specific oil palm cross evidenced common or specific QTLs in E. guineensis and E. oleífera. Genes related to palm oil fatty acids were investigated using full-length cDNA libraries and mRNA extracts from the mesocarp of developing fruits in each Elaeis species represented by four genetic pools. Expression of 113 key Elaeis genes or putative regulation factors of de
novo fatty acid and triacylglyceroi pathways were characterized at species level by in silico transcript profiling
and Real Time RT-qPCR analysis. Genes were mapped on the pseudo-backcross using 180 intra-gene SNP markers. Forty-eight genes were differentially expressed between Elaeis species, with 94% over expressed in
E. guineensis. The EgAP2-2 ( WRI1-like) transcription factor might influence the start of the de novo fatty acid
pathway by main action only on the MAT and KAS III genes, as independent in expression from other genes
