Figure S1

(1)

VPH 1 mt G F P SEC 63 SEC 7 SED 5 transmission

fluorescence yy∆∆ fluorescence WT transmission

(2)

0 WT yy∆∆ 10 20 45 5 LY WT yy∆∆

Figure S2

a

b

(3)

Figure S3

a

b

WT yy∆∆ vps4∆ ypc1∆vps4∆ WT

top

_{1 2 3 4 5 6}

bottom

CAN1 GAS1 SEC63 yy ∆ ∆ CAN1 GAS1 SEC63

(4)

MMA - + - + - + - + AbA - + - + yy∆∆ WT 1 2 3 4 5 6 7 8 PHS-C26 DHS-C26

a

b

WT yy∆∆ 1 2 3 4 5 6 7 8 MMA - + - + - + - + AbA - + - + Cers PE PC PS M(IP)2C IPC-C MIPC

Figure S4

* * * * * * * * PHS- C26-OH DHS- C26-OH * * * *

(5)

Figure S5

0 50 100 150 200 1 2 3 4 5 6 100 - 0 L-sp hi ng osi ne D -sp hi ng osi ne N ,N -D iMe -D -e ryt hro -sp hi ng osi ne PH S [ 3H ]-ce ra mi de (% o f st an da rd ) D-th ré o-D H S L-th ré o-D H S [lo g]10 % o f s tan dar d re ac =o n

a

b

(6)

!" #" $" %" &" '!" '#" '$" !" '!" #!" (!" $!" !" #$$ %&'()* +& ,-*.%&/01& )*" +,-'." +/-'." +,-'.+/-'." !" #" $" %" &" '" (" )" !" #!" $!" %!" &!" !" #$$ %&'()* +& ,-*.%&/01&& *+" ,-.#/" ,0.#/" ,-.#/,0.#/" YPD YPEthanol !" #" $" %" &" '" (" )" *" !" #!" $!" %!" &!" !" #$$ %&'()* +& ,-*.%&/01& +," -./#0" -1/#0" -./#0-1/#0" YPGlycerol YPLactate !" #" $" %" &" '" (" !" #!" $!" %!" &!" !" #$$ %&'()* +& ,-*.%&/01& )*" +,-#." +/-#." +,-#.+/-#."

Figure S6

wt ypc1∆ ydc1∆ yy∆∆ wt ypc1∆ ydc1∆ yy∆∆ wt ypc1∆ ydc1∆ yy∆∆ wt ypc1∆ ydc1∆ yy∆∆

(7)

Figure S7

cytosol 100 000 g pellet

12 000 g pellet

(8)

0 20 40 60 80 100 120 0 1 3 7 10 14 17 21 Vi ab ili ty , % o f d ay 0 Time, days wt ydc1∆ ypc1∆

Figure S8

(9)