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Transcriptional profiling of pseudomonas aeruginosa and staphylococcus aureus during in vitro co-culture

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Transcriptional profiling of pseudomonas aeruginosa and staphylococcus aureus during in vitro co-culture

TOGNON, Mikael, et al.

Abstract

Co-colonization by Pseudomonas aeruginosa and Staphylococcus aureus is frequent in cystic fibrosis patients. Polymicrobial infections involve both detrimental and beneficial interactions between different bacterial species. Such interactions potentially indirectly impact the human host through virulence, antibiosis and immunomodulation.

TOGNON, Mikael, et al. Transcriptional profiling of pseudomonas aeruginosa and

staphylococcus aureus during in vitro co-culture. BMC Genomics, 2019, vol. 20, no. 1, p. 30

PMID : 30630428

DOI : 10.1186/s12864-018-5398-y

Available at:

http://archive-ouverte.unige.ch/unige:120155

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Fig. S2 Comparison of the fold-changes (log2 base) obtained by RNA sequencing and qRT-PCR for three genes that were up- or down-regulated in each organism in response to the competitor. A statistically significant correlation (R2 = 0.895, p < 0.05) was found for the 12 genes tested. P.

aeruginosa genes (filled symbols), S. aureus genes (open symbols). Data points represent the average of the three replicate samples. The package

‘gplots’ of R (https://cran.r-project.org/web/packages/gplots/index.html) was used for the regression analysis.

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