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Phylogeography and genetic differentiation along the distributional range of the orchid Epidendrum fulgens: a Neotropical coastal species not restricted to glacial refugia

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Phylogeography and genetic differentiation along the distributional range of the orchid Epidendrum fulgens: a Neotropical coastal species not restricted to glacial refugia

Fábio Pinheiro, Fábio de Barros, Clarisse Palma-Silva, Michael F. Fay, Christian Lexer and Salvatore Cozzolino Journal of Biogeography

Appendix S1 Overview of the main types of habitats where Epidendrum fulgens can be found.

Appendix S2 Epidendrum fulgens natural growing conditions and flower morphology.

Appendix S3 Magnitude of ΔK from STRUCTURE analysis as a function of K.

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Appendix S1 Overview of the main types of habitats where Epidendrum fulgens can be found. (a) Sand dune vegetation of the Imbituba population, formed by Pleistocenic sand deposits, Santa Catarina state, Brazil; (b) Sand dune vegetation from the Superagui population, formed by recent Holocene sand deposits, Paraná state, Brazil; (c) Morro do Cabrito arenitic outcrop, where many Brazilian Atlantic rain forest species have their southern limits, Rio Grande do Sul state, Brazil; Morro São Pedro granitic bedrock, composed of a mosaic of forest and grassland plant communities, Rio Grande do Sul state, Brazil.

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Appendix S2 Epidendrum fulgens plants growing in grassland (a) and open scrub vegetation (b); many plants can be also found growing direct on uncovered sand (c); (d) detail of flower morphology.

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Appendix S3 Detection of the number of groups in the whole nuclear microsatellite data set of Epidendrum fulgens. The magnitude of ΔK from

STRUCTURE analysis as a function of K (details in Materials and Methods) was calculated following the simulations described by Evanno et al.

(2005). The modal value of these distributions indicates the true K or the uppermost level of structure, here two genetic clusters.

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