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Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression.

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(1)Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression. Stéphane Gesta, Karine Lolmède, Danièle Daviaud, Michel Berlan, Anne Bouloumié, Max Lafontan, Philippe Valet, Jean-Sébastien Saulnier-Blache. To cite this version: Stéphane Gesta, Karine Lolmède, Danièle Daviaud, Michel Berlan, Anne Bouloumié, et al.. Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression.. Horm Metab Res, 2003, 35 (3), pp.158-63. �10.1055/s-2003-39070�. �inserm-00110141�. HAL Id: inserm-00110141 https://www.hal.inserm.fr/inserm-00110141 Submitted on 4 Sep 2009. HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés..

(2) Hal author manuscript Horm Metab Res 03/2003; 35(3): 158-63. 

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(439) G=Gi=„ Ž L%KMd . . Hal author manuscript. — deGiF6@A8n@e8*<)=QoWXd‰d*>)Fn•e™%—  NnY ]G@r€T‹ƒLB.:  v}B.} ~ —NnY D ‹&LB — NnY Y „2‹ƒL  –B.  v YZB. L.Q

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(573) d8*>)Fn™ Ž ;–TG=œ8e;> [1WXL8€W2F>NP>

(574) QfG <v.‘;>@>-Q<18*<[

(575) K=dAGFNP>

(576) Q 8e;2<188e;$>[1WXL8€W2F>KMd. <

(577) QG†:2K%@> 8eG€@@AWX>>)c:2L <

(578) =58@  <5@ <@@K2[

(579) G<18*>

(580) Q TG8e; <

(581) = G=$[)F>

(582) <5@6>G= xn’. α. :FK2Q5WX[ 8eGK=%vRT<!8*< <

(583) L@'K. @‰;$K >

(584) QP<_=PG=2[)F>

(585) <@>Gi=U<

(586) =<+>)FKo2G[„ML”2[

(587) KML†”X@G€@aG=U>Cc$:MLq<)=58@ v‘;Gs@L <$@e8.Ko%@'>)FAV2<!8‰GK= T<@.F>

(588) NTG=MG€@6[

(589) >

(590) =58 Kd4<k;”$:2K$c2G ["@e8*<18*>v.   

(591)   !" $% !"  & ' IaG :2KML†”%@6Gs@ZG€@b< Vf>)F‰” GNT:2KFA8*<)=+8œN >18*<1o2K2LiG [ dW%=2[!8eGK= Kd <

(592) QfG:2K%@> 8eG@!@eWX> T;G [

(593) ; [+<_= oX> F>)„WXL<18*>CQ o”ZQGid*d‰>)F'>_=+8;KF‰NTK=2>5@T<

(594) =$Q =$>1W2FK8€F<)=2@N(G8€8*>,F@T•A™  – v w = KFQ>)Fk8sK Q>8*>)F‰NTG=2> T;$>18e;2>)F [1WXL8€W2F>PKfdz<

(595) QG:2K@'> 8eG€@@eW >P>Cc$:ML <

(596) =58@^[

(597) KW%LŠQ G=d‰L†WX>

(598) =[+> 8e;>(G=58*>,„FHG8A” KMdƒ[

(599) >

(600) L L]d€W%=2[!8eGKf=$B8e;>pLG:2KML†”+8‰G [ ,.

(601) <

(602) [18eG†V2G8e” Kfdƒ<CQfG:2K2[C”+8*>5@^Gs@'KML<18*>CQ d€FKMN 8e;>(>!c:2L<

(603) =+8@ <5@NT>+<5@eW2F>

(604) Q o2>

(605) deKF>(<,=Q<

(606) d8*>)F Ž ;[1WXL8€W2F>uv ;$>_=U[

(607) KMNn:2<,F>

(608) Q  G8‰;n8*GN(>  KMdl[1W%L8€W2F>

(609) B%<n@G„M=G d‰G [

(610) <

(611) =58.G=[)F'>

(612) <@>• v  deKMLQ–aG=(oX<5@'<_L L Gi:2K2LŠ”X@G€@ P<@ Ko%@>,FAV2>

(613) Qp<

(614) d8*>)F Ž ;P[1WXL8€W2F>•s’hGi„W2F'> `–!v w =P<

(615) QQG8*GKM=$Bf8e;>[

(616) K=[

(617) >

(618) =58eF'<18eGK=r‰Q>):2>

(619) =$Q>_=+8.<

(620) [!8eGŠVf<18*GKf=PKMd Hal author manuscript. LG:2KML†”X@Gs@o” 8e;>. β. r‰<

(621) QF>)=$>)F‰„2G [ <,„2Kf=G€@A8 G@K:FK58*>)F*>)=K2L F'>CVf>

(622) <

(623) L >

(624) Q =K @G†„M=G d‰G [

(625) <

(626) =58 Q>

(627) [)F>C<5@> G=. NP<)c2GNP<

(628) L(>

(629) ded‰>+[18  G8e; =K [);<

(630) =u„2>$@ G=yƒ‹k—  •  v ™™ <)=$Q  v š . Y <18p8eGNP>  <

(631) =Q 8*GNP> Ž ;. F>5@*:2>

(632) [18eG†VX>

(633) Li”u–ƒ•A’hG„W2F> \z–!v w =p<CQQfG8*GK=B <

(634) [18*G†V2<18*GK=pKdaLG:2KML†”%@6Gs@ao”P8e;>n<CQM>

(635) =”L”2L [_”2[

(636) L <5@'> <C[18‰G†Vf<18*KF deKF@2KMLG=  <$@ =$K$8-<,L8*>)F>

(637) Q •e=K8 @‰;K E=–!v w =ˆ:2<)F<

(638) LL >

(639) LB(=K @G†„2=Gd‰G [

(640) <

(641) =+8-<_L8*>)F<18eGK= Gi= 8e;$>ŒN(>C<5=. inserm-00110141, version 1. QG<

(642) N(>!8*>)FaKd‘G€@KML <18*>CQ(<

(643) QG:2KM[)”+8*>5@]of>18  >

(644) >)=  •   v   v ™ N = ‘—+–a<

(645) =Q Ž ;P•   v ™  v  Y = ‘—– . . [1WXL8€W2F>  <5@ K$oX@6>)FAV2>CQ%v".;>5@6>ŒQ<!8*< @;K T>CQ 8e;<18P8e;>ŒdW%=$[18*GKf=$<

(646) L(•‰G€v>vLG:2KML†”+8‰G [ :F'KM[

(647) >+@@‰– <_=2Q NTKF‰:2;KLKf„2G [

(648) <

(649) LpG=58*>)„FG8e” KMd-Gs@'KML <18*>

(650) Q <

(651) QG†:2KM[)”+8*>5@ T>,F>Œ=K8 QF<,NT<18*G[+<_LL” <

(652) L8*>)F'>

(653) Q‚o” :FGN(<,FH” [1WXL8€W2F>zKfdh<

(654) QG:2K@'>&8eG@@eW >>1c$:ML <

(655) =58@ v.   

(656)  %!       w = KFQ>)Fœ8sK Q>18*>!F‰NTG=2> T;$>18e;>,FŒ[1WXL8€W2F> Kd <CQfG:XK@'> 8eG@!@AWX> >)c:2L<

(657) =+8@Z[

(658) KW%LQ G=Md‰L†WX>

(659) =[+> <

(660) QG†:2K2[C”+8*> „2>_=$> >)c:F>5@!@GŠKf=$B&8e;>œF>

(661) L <18*GŠVf>œL >CVf>

(662) LKMd @'>CVf>)F<

(663) LN {x(`T@P<5@<

(664) =<

(665) L”+>

(666) QG= G€@'KML<18*>

(667) Q <

(668) QG†:2K2[C”+8*>5@ v%`n@"@;$KE=Gi=’4G„W2F>n™2B :FHGNP<)F‰”-[1WXL8€W2F> <5@z<@@K2[

(669) G <18*>

(670) QTG8e;<TQF<

(671) NP<18‰GŠ[ <

(672) =$QJ8eGN(>Cr Q>):2>)=$QM>

(673) =+8 Q>

(674) [,F>

(675) <5@'> G= 8e;><

(676) NTKW2=58-Kd @'>CVX>,F<

(677) L(Nn{x(` @ >

(678) =$[

(679) KMQG=„ deKF :F‰K58*>,Gi=2@ @‰:X>

(680) [

(681) Gd‰G [5<

(682) LL” >1c$:F>5@!@>CQJG=p<CQfG:2K2[C”+8*>“`QfG:2K2[)”58*>IaG†:2G Q \G=2QGi=„(ghF*K58*>)G=p•‰`kI.\&gh–BMI.G†:2K:FK58*>)G=TIaG:2<@>n•€I‘gI.–'B  KfF‰N Kf=$> 72>

(683) =%@G8‰G†VX> IG†:X<5@> •sU7I.– •s’hG„W2F>™M`–B<

(684) =Q‚gl>)F'K$c2G€@K2NT> ghFKMLGd*>)F<18*GK= `[!8‰G†V2<!8‰G=„ {^>

(685) [

(686) >):8*KF γrA™-•qg gh`k{ γ™–z•s’hG†„W2F>n™\z–!v%‘;>5@6>TQK n=$rAF>)„WXL<18*GK=%@ T>,F>T<5@@'KM[

(687) G<18*>CQ TG8e;< QF'<_NP<18eG [ G=2[)F>

(688) <@'> G= 8e;$> L >)V2>

(689) LbKMd @>)V2>)F'<_LbK8e;>)F Nn{x(`T@,“ β r‰<

(690) [8eGi= •s’hGi„W2F'> ™M`–BZ x ’ αBZ?"LWX[

(691) K%@>. 8eF<)=2@‰:2KFs8*>,F‰r •‰? LW8*r –z<)=QJE”:2Kc2G < w =Q5WX[C>

(692) QJ’l<

(693) [18*KFr. α. •€ w ’r α–zN"{x(`T@•s’hG„W2F>T™\k– v w =(8e;>. @<

(694) NP> 8eGNP>J=$K V2<,FHG <18*GK= Gi=8e;>pL>)VX>

(695) L.KMdz[)”2[

(696) LK:M;G LG = Nn{x(`  <$@ K$oX@>)F‰V2>CQ •€’4G„W2F>(™\z–!vh‘;>5@6> F>5@eW%L8@@;K T>

(697) Q(8e;<!8a:F‰GNP<)F‰”-[1WXL8€W2F>TKda<

(698) QG:2K@'>"8‰G@!@eWX>T>Cc$:ML <

(699) =58@  <5@z<@@'KM[

(700) G <18*>

(701) QTG8e;GNT:2KfFA8*<,=58 <

(702) L8*>)F<!8eGKf=2@G= „X>

(703) =>P>Cc$:F>5@6@GKM=%vMR K n=$rAF>)„WXL<18*GK=Kdƒ` I‘\ƒglBp7IBI‘g IB‘<)=$QJg g4`{ γ™ N"{x(`T@ .

(704)  <5@pF>

(705) NTG=MG€@6[

(706) >

(707) =58 KfdP<

(708) =Gi=2Q5WX[!8‰GK=KdP<

(709) QfG:2K2[)”58*> Q>CQfGded‰>)F'>_=+8‰GŠ<18‰GŠKf=Œ:F'KM[

(710) >+@@!va|E:2rAF>)„WXL<18*GK=M@KMd ?"LW8*r <,=Q" w ’hr. α. N"{x(`T@  >)F>kF>)NTG=2Gs@6[C>_=+8hKd4<k;”$:2K$c2G ["@e8*<18*>v. Hal author manuscript.     

(711)  

(712)   .   !"  &%         ` @ Q>5@[,FHG†oX>

(713) Q <)ofK$Vf>

(714) B"8e;>Œ[1WXL8€W2F>bKfd<

(715) QG:2K@'> 8eG@!@‰WX>Œ>!c:2L<

(716) =+8@  <5@ <@@'KM[

(717) G<!8*>

(718) Q TG8e; <5= <

(719) [

(720) [!W%N"WXL <18*GKf=UKfdlxT’ α G=T8e;>n[1WXL8€W2F>N(>CQfG†WXNpv];Ge@a<C[

(721) [1WXN WXL <18*GKf=  <@a<@6@K2[_GŠ<18*>

(722) Q  G8‰;P<k8‰GNP>

(723) r Q>):2>)=$QM>

(724) =+8aG=[,F>

(725) <@>TGi=-<

(726) QG:2KM[)”+8*>Txn’ α N"{ƒxP` L>)VX>

(727) L4•€’4G„W2F>n™\z–!vXxn’ α G€@z< [)”+8*K MG=> 2=K T=. inserm-00110141, version 1. 8eK->1c2>)Fe8ƒ<n:2K T>)F*dW%LhGi=;2G†o%G8*KfF‰”>

(728) ded‰>+[18 K=<

(729) QfG :2KM[)”+8*>(QGdAd*>)F>

(730) =58*G<18*GK=• ˜r  –!v w =KFQ>)Fa8eK(8*>5@€8]8e;$> :2K58*>)=+8eG<

(731) L‘Gi=VfKML†VX>

(732) N(>

(733) =+8ƒKda xn’ α Gi=-[1WXL8€W2F>

(734) r‰G=Q5WX[C>

(735) Q<

(736) QG†:2K2[C”+8*>TQ>

(737) QGdAd*>)F>

(738) =58*G<18*GK=Bh<

(739) QfG:2K%@>"8eG@!@eW > >1c$:ML <

(740) =58@ T>)F'>n[1WXL8€W2F>

(741) QpG= 8e;>:F>@>

(742) =[

(743) >EKMd.<":2;K%@‰:;K2QG>@A8*>)F<+@>nG=;GŠoXG8*KFB <"de<

(744) NTGLi”JKMd.NTKML >

(745) [1WXL€>. E;G[

(746) ;$Blo” G=2[)F>

(747) <@G=„œG=+8eF<

(748) [

(749) >

(750) LL†W%L <)F[

(751) `YœglB]G=;G†o2G8 x ’ α :FK2Q5WX[ 8eGŠKf= •e™ BS™™–!v%’hKFƒ8e;>(:F>5@'>)=58 @A8€WXQ5” P> W @'>

(752) Q 8e;$> :2;K%@‰:2;K2QG>5@A8*>,F<5@>ZGi=;2G†o%G8*KfFG€@KoW8€”L NP>18e;”2L†cX<_=+8e;G= • w \ƒY "–B T;G [_;ˆ;<@. :F>1V2GŠK$W @L” of> Q>

(753) NTK2=M@A8eF'<18*>

(754) Q 8eK @AW2::F>5@6@ >)=QK„2>)=$K$W @ :FK2Q5WX[8eGKf= Kd x ’ :F>

(755) <CQfG†:2K2[)”+8*>5@-•A™2}–!v w ƒ\ Y  • , . α. G= ;5WXNP<5=. Y – @A8eF*Kf=u„ML†”ˆF>

(756) Q5WX[

(757) >CQ 8e;>Z<

(758) [

(759) [!W%NnWXL<!8‰GK=ˆKMdp xn’. α. G= 8e;2>. [1WXL8€W2F>nNP>

(760) QGŠWXN <

(761) d8*>)F Ž ;[1WXL8€W2F>T•‰}}%v  }%vŠ—T:„ eNTL q„Kd‘8eGs@6@AW>n=‘—+–!v w \&Y  <

(762) L€@K :F>!V2>

(763) =58*>

(764) Q 8e;> G=2[)F>

(765) <+@> G=ˆ<

(766) QGi:2K2[)”+8*> xn’. α. N {x(` L >)V2>

(767) LEK$oX@6>)FAVf>

(768) Q <

(769) d8*>)F Ž ;[1WXL8€W2F> •A’hG„W2F> }f`– v w =. :2<)F<

(770) LL >

(771) LB w \ƒY  :F>!V2>

(772) =58*>

(773) Q 8e;$>"F>

(774) Q5WX[8eGK=pG=p` I‘\ƒglBMp7Ib•s’hG†„W2F>E}5\k– <)=Q g g‘`k{ γ™•€’4G„W2F>E}f`–. Nn{x(` L >)VX>

(775) L€@B <5@

(776)  >

(777) LLh<5@.8e;>nG=2[)F>

(778) <@>nG= β r‰<

(779) [18eG=pNT{axP` L>)VX>

(780) L •A’hG„W2F>n}5\z–B K$oX@>)FeV2>

(781) Qp<

(782) d8*>)F Ž ;. [1WXL8€W2F>vlS;$>J:FK58*>C[ 8eG†V2>>

(783) dAd*>+[18„2>,=>)F<!8*>

(784) Q o” w \ƒY   <5@E<_L€@'K K$oX@>)F‰V2>CQ TG8*;8e;>@<

(785) NP>>)c8*>)=2Q. TG8e;U<)„2>

(786) =58@.8e;<18.G=[,F>+<5@>n[

(787) `YœgZ@AWX[

(788) ;U<$@.8e;>n<CQ>_=”2L”2Lh[C”f[

(789) L<@'>n<

(790) [18*G†V2<18*KFadeKF@$KfLG=•‰=K8ƒ@‰;K E=–!v ‘;>5@'>Q<18*<"@‰;$K >

(791) Q"8e;>kG=V2K2LŠVf>

(792) N(>

(793) =+8‘Kdh[)`Yœg G=T[)WXL8€W2F>

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