THEMATICd SYPOSIUM : S 4 :EnvironmentaldMicrobiologydanddBiotechnology POSTERd PRESENTATIONSd: P 1 – P 85
Biodegradationdofdolivedmilldwastewaterdbydnon-conven-tionaldyeasts
Gonçalves,dC.,dAguedo,dM.danddBelo,dI.d InstitutedfordBiotechnologydanddBioengineering,dCentredfordBiologicaldEng.,dUniversidadeddo Minho,dBraga cristianammg@deb.uminho.pt OlivedmilldindustrydisdverydimportantdindMediterraneandcountries,dwhichdaredre-sponsibledfordalmostdalldthedolivedoildsolddworldwide.dPortugaldisdonedofdthedten majord producers.d Olived oild productiond resultsd ond ad larged amountd ofd liquid waste,d whichd representsd ad majord environmentald problem.d Thed qualityd and quantitydofdolivedmilldwastewaterd(OMW)dconstituentsddependsdondmanydfac-tors,dsuchdas,dtypedofdolives,dtypedofdsoildanddproductiondprocessd(D’Annibaldet al.,d2006,dRoigdetdal.,d2006).dThedlargedvarietydofdcomponentsdfounddindOMW (suchdasdlipidsdanddphenolicdcompounds)ddifficultdtheirdtreatmentd(Niaounakis, 2004).dThedaimdofdthedpresentdworkdwasdthedcomparisondofdthedpotentialduse ofddifferentdnon-conventionaldyeasts,dtodgrowdanddtodproducedhigh-valuedcom-poundsd fromd OMW,d whiled degradingd andd detoxifyingd thisd waste.OMWd from differentd regionsd ofd Portugald wered collectedd andd characterized.d OMWd based mediumdweredprepareddbydsupplementingdwithdyeastdextractdanddammonium chlorided proportionallyd tod itsd organicd load.d Bothd strainsd usedd ind thisd work (Yarrowiadlipolytica W29danddCandidadrugosa PYCCd3238),dweredabledtodgrow indOMWdwithdchemicaldoxygenddemandd(COD)drangingdfromd20 g/Ldtod200 g/L, todconsumedalmostdalldofdthedsugarsdpresentdindthedmediad(betweend64 %dand 91 %)danddtodsignificantlydreducedCODdvaluesd(betweend30 %dandd62 %).dHow-ever,dthedY.dlipolytica straindrevealeddtodbedmoredefficientdthandthedC.drugosa straindfordthedOMWdutilizationdandddegradation.1.dD’Annibale,dA.detdal.d(2006)dBioresourcedTechnology 97:1828-1833. 2.dRoig,dA.detdal.d(2006)dWastedManagement 26:960-969.
3.dNiaounakis,dM.,dHalvadakis,dC. P.d(2004)dTypothito,dGeorgedDardanos,dGreece,d14:430
Used ofd morphologicald andd physiologicald informationd to
identifydchangesdindanaerobicdgranulardsludgedduringdor-ganicdoverloadsd
Costa,dJ. C.,dMoita,dI.,dAbreu,dA. A.,dFerreira,dE. C.danddAlves,dM. M.
IBBd -d Instituted ford Biotechnologyd andd Bioengineering,d Centred ofd Biologicald Engineering, UniversitydofdMinho,dBraga carloscosta@deb.uminho.pt Fourdlab-scaledExpandeddGranulardSludgedBlanketdreactorsdoperatingdwithdan OrganicdLoadingdRated(OLR)dofd5kgCOD.m-3.d-1anddHydraulicdRetentiondTime (HRT)dofd8 hdweredoperateddtodapplydfourdorganicdshockdloads.dIndreactorsdR1 anddR2,dthedOLRdwasdincreaseddtod18kgCOD.m-3.d-1 ,dthroughdethanoldconcen-trationdanddinfluentdflowdrated(HRTdofd2.5 h)dincreases.dIndreactorsdR3danddR4, thedOLRdwasdincreaseddtod50kgCOD.m-3.d-1 duringd3dandd16ddays,drespec-tively.dNodsignificantdeffectsdwereddetecteddindperformancedofdR1.dIndR2dthe ChemicaldOxygendDemandd(COD)dremovaldefficiencyddroppeddfromd94dtod72 %, although,dnodsignificantdchangesdoccurreddindthedSpecificdMethanogenicdActiv- ityd(SMA).dGranulesderosiondwasdobserveddindR1d(areadofdgranulesdwithdEquiv-alentd Diameterd (Deq) > 1 mmd decreasedd fromd 90d tod 70 %).d Whiled ind R2d the washoutdphenomenondwasdimmediatedindR1dthisdwasdmoredsevere.dR3dand R4dshowedddecreasesdofdefficiencydfromd90dtod30 %.dThedSMAdwasdinhibited duringd adaptingd phased (24 h).d Afterwards,d ind R3,d thed SMAd wasd recovered. RelativelydtodR4,dalthoughdSMAdindH2/CO2recovered,dthedSMAdindacetatedwas reduceddduringdthedexposuredtime,dmeaningdthatdacetoclasticdbacteriadwere inhibited.d Extendd ofd exposured timed ind R4d provokedd acidogenesisd inhibition afterd144 h.dGranulesdfragmentation,dtranslateddbyd45 %dreductiondindareadof aggregatesdwithdDeq > 1 mm,dwasdobserved.dFilamentsdreleasedanddwashout ofdbiomassdoccurred.dIndR4dadnewddynamicdindgranulesdstructuredanddbiomass washoutd wasd obtained;d onlyd denserd granulesd remainedd insided thed reactor. Smalldanddfluffierdgranulesdweredpresentdatdthedenddofdshockdloads. Acknowledgements: Wed gratefuld acknowledged thed financiald supportd tod J.C.d Costad throughd thed grant
SFRH/BD/13317/2003,d tod I.d Moitad andd A.A.d Abreud throughd thed projectsd POCI/AMB/60141/2004d and POCTI/BIO/37934/2001,drespectively,dfromdthedFundaçãodparadadCiênciadedadTecnologiad(Portugal). 151 R E F E R E N C E S4 /P56 R E F E R E N C E S4 /P55
