ii J. Anim. Sci. Vol. 91, E-Suppl. 2/J. Dairy Sci. Vol. 96, E-Suppl. 1 LB4 Effects of glucuronic acid and N-acetyl-D-glucosamine on
the in vitro fertilization of porcine oocytes..6FKPLGW .'DOWRQ & 'XUIH\ . /HPRQ$ 0HOOR DQG % ' :KLWDNHU University of
Findlay, Findlay, OH.
Pig oocytes fertilized in vitro H[SHULHQFHKLJKSRO\VSHUPLFSHQHWUDWLRQ UDWHVGXHWRLQDGHTXDWHFRUWLFDOJUDQXOHH[RF\WRVLV7KHREMHFWLYHZDV to minimize polyspermic penetration by increasing the perivitelline VSDFH 396 WKLFNQHVV WKURXJK VXSSOHPHQWDWLRQ RI LWV FRPSRQHQWV JOXFXURQLF DFLG *$ DQG N-acetyl-DJOXFRVDPLQH *OF1$F GXULQJ
PDWXUDWLRQ2RF\WHVQ ZHUHVXSSOHPHQWHGGXULQJWKHODVW h of maturation with either 0.01 mM*$PM*OF1$FPM *$DQG*OF1$FRUPM*$DQG*OF1$FDQGWKHQHYDOXDWHGIRU ]RQDSHOOXFLGDDQG396WKLFNQHVV,QWUDFHOOXODUJOXWDWKLRQHFRQFHQWUD-WLRQVZHUHGHWHUPLQHGDIWHUPDWXUDWLRQLQDSRUWLRQRIWKHRRF\WHVQ DQGWKHUHPDLQLQJRRF\WHVZHUHIHUWLOL]HG$WKSRVWIHUWLOL]DWLRQ RRF\WHVQ ZHUHHYDOXDWHGIRUIHUWLOL]DWLRQDQGFRUWLFDOJUDQXOH characteristics and the remaining putative embryos were cultured and HYDOXDWHG IRU FOHDYDJH DQG EODVWRF\VW IRUPDWLRQ DW K DQG K SRVWIHUWLOL]DWLRQ 7KHUH ZHUH QR VLJQL¿FDQW GLIIHUHQFHV EHWZHHQ WKH WUHDWPHQWVIRU]RQDSHOOXFLGDWKLFNQHVVLQWUDFHOOXODUJOXWDWKLRQHFRQ-FHQWUDWLRQVSHQHWUDWLRQUDWHVRUPDOHSURQXFOHDUIRUPDWLRQ7KH396 WKLFNQHVVZDVVLJQL¿FDQWO\WKLFNHUPLQDOOWUHDWPHQWVFRPSDUHG WRWKHFRQWUROȝP2RF\WHVVXSSOHPHQWHGZLWKPM *$ȝPRUPM*$DQG*OF1$FȝP KDGVLJQL¿FDQWO\WKLFNHU396PWKDQPM*OF1$F ȝPRUPM*$DQG*OF1$FVXSSOHPHQWHGRRF\WHV ȝP2RF\WHVVXSSOHPHQWHGZLWKPM*$RUPM*$DQG *OF1$FKDGVLJQL¿FDQWO\PRUHFRUWLFDOJUDQXOHH[RF\WRVLVP FRPSDUHGZLWKWKHRWKHUWUHDWPHQWV2RF\WHVVXSSOHPHQWHGZLWK*$ KDGVLJQL¿FDQWO\ORZHULQFLGHQFHVPRISRO\VSHUPLFSHQHWUD-WLRQFRPSDUHGZLWKWKHFRQWURORUPM*OF1$F DQGVLJQL¿FDQWO\KLJKHUUDWHVPRIFOHDYDJH DQG EODVWRF\VW IRUPDWLRQ E\ DQG K SRVWIHUWLOL]DWLRQ 7KHVH REVHUYDWLRQVLQGLFDWHWKDWVXSSOHPHQWLQJ*$GXULQJRRF\WHPDWXUDWLRQ GHFUHDVHV SRO\VSHUPLF SHQHWUDWLRQ E\ LQFUHDVLQJ 396 WKLFNQHVV DQG FRUWLFDOJUDQXOHH[RF\WRVLVLQSLJV
Key Words: cortical granules, polyspermy, pig
LB5 Direct use of MACE EBV in the Walloon single-step Bayesian genomic evaluation system. -9DQGHQSODV1,2, F. Colinet1,
3)DX[169DQGHULFN1DQG1*HQJOHU 11ULg-GxABT, Gembloux,
Belgium, 2FNRS, Brussels, Belgium.
6LQJOHVWHSJHQRPLFHYDOXDWLRQVVV*%/83VKRXOGUHGXFHSRWHQWLDO biases in the estimation of genomically enhanced breeding values *(%9E\WKHVLPXOWDQHRXVFRPELQDWLRQRIJHQRPLFSHGLJUHHDQG SKHQRW\SLFLQIRUPDWLRQDOVREHFDXVHIHZHUDSSUR[LPDWLRQVDUHPDGH than in multi-step methods. However, most current genomic evaluation systems are multi-step, relying heavily on the use of multiple across-FRXQWU\HYDOXDWLRQ0$&(UHVXOWVDVWKHSULPDU\VRXUFHRIIRUHLJQ SKHQRW\SLFLQIRUPDWLRQ5HFHQWO\DQHHGZDVLGHQWL¿HGWRGHYHORSGLUHFW XVHRI0$&(HVWLPDWHGEUHHGLQJYDOXHV0$&((%9LQVV*%/83 Therefore, the aim of this report is to show the development and practical use of an innovative method that considers simultaneously all avail-able genotype, pedigree, local, and foreign information in a genomic evaluation system. The developed method is a Bayesian approach DVVRFLDWHGZLWKVV*%/837KHPHWKRGDOORZVDFRUUHFWSURSDJDWLRQRI LQIRUPDWLRQDQGDYRLGVPXOWLSOHFRQVLGHUDWLRQVRIFRQWULEXWLRQV,WDOVR allows adding and subtracting contributions from different information VRXUFHVIRUH[DPSOHWRDYRLGGRXEOHFRXQWLQJRIORFDOLQIRUPDWLRQ DOUHDG\FRQWULEXWLQJWR0$&((%9$QRWKHUDGYDQWDJHRIWKLV%D\HVLDQ
approach is that it creates a model equivalent to a complete one directly combining all available information without any additional deregression VWHSV7KHDSSURDFKZDVVHWXSXVLQJ+ROVWHLQDQLPDOV ZLWKD:DOORRQ(%9DQGEXOOVZLWK0$&((%9$WRWDORI FRZV DQG EXOOV FRQWULEXWHG WR WKH JHQRPLF UHODWLRQVKLS PDWUL[ WKDW ZDVFRPELQHGZLWKWKHSHGLJUHHEDVHGQXPHUDWRUUHODWLRQVKLSPDWUL[ 3KHQRW\SLFLQIRUPDWLRQZDVDGGHGWKURXJK:DOORRQ(%9DQG0$&( (%9/RFDOLQIRUPDWLRQDOVRLQFOXGHGLQ0$&((%9ZDVGLVFRXQWHG IRUE\VXEWUDFWLQJLQWKH%D\HVLDQLQWHJUDWLRQSURFHVVWKHORFDO(%9 VHQWWR,QWHUEXOO7KLVJHQRPLFHYDOXDWLRQV\VWHPSDVVHGWKH,QWHUEXOO *(%9WHVWVIRUPLONIDWDQGSURWHLQ\LHOGVDQGWKHPDMRULW\RIW\SH traits in February 2013. This approach has the potential to improve current genomic prediction strategies also in beef and in other species HJVZLQH
Key Words:%D\HVLDQLQWHJUDWLRQ0$&(JHQRPLFSUHGLFWLRQ
LB6 Genetic mechanisms that contribute to differences in beef tenderness following electrical stimulation. 5 1 9DXJKQ $ . 7RUUHV.-.RFKDQ5.0LOOHU&$*LOO$'+HUULQJ'* 5LOH\-26DQGHUV-:6DYHOO7+:HOVKDQG3.5LJJVTexas
A&M University, College Station.
%HHIWHQGHUQHVVLVYDOXHGE\FRQVXPHUVDQGLQÀXHQFHGE\ERWKHQYLURQ-mental and genetic factors. Postmortem treatment by electrical stimu-ODWLRQ(6LQFUHDVHVWHQGHUQHVVDQGUHGXFHVEXWGRHVQRWHOLPLQDWH YDULDWLRQDPRQJFDUFDVVHV7KHSXUSRVHRIWKLVVWXG\ZDVWRH[DPLQH JHQHWLFIDFWRUVWKDWLQÀXHQFHWHQGHUQHVVSDUWLFXODUO\LQSRVW(6EHHI 6NHOHWDOPXVFOHVDPSOHVZHUHFROOHFWHGLPPHGLDWHO\DIWHUVODXJKWHUIURP crossbred F21HOORUH$QJXVVWHHUV:DUQHU%UDW]OHUVKHDUIRUFH:%6)
measurements following 14 d of aging were used as an objective measure RIWHQGHUQHVV0LFURDUUD\DQDO\VLVRIVDPSOHVIURPVWHHUVFKRVHQ IRUGLYHUJHQWUHVSRQVHWR(6ZDVXVHGWRLGHQWLI\QHWZRUNVRIJHQHV ZLWKVLJQL¿FDQWO\GLIIHUHQWJHQHH[SUHVVLRQEHWZHHQWHQGHUQHVVJURXSV DQGWRLGHQWLI\VLJQL¿FDQWO\HQULFKHGVLJQDOLQJSDWKZD\V,QDGGLWLRQ 613KDSORW\SHEORFNVHQFRPSDVVLQJJHQHVRILQWHUHVWZHUHFRQVWUXFWHG WRH[DPLQHSDUHQWDQGEUHHGRIRULJLQHIIHFWV7KHH[WUDFHOOXODUPDWUL[ (&0DQGIRFDODGKHVLRQSDWKZD\VZHUHHQULFKHGLQWKHPLFURDUUD\ assay. From this pathway, a total of 40 genes were assayed by qRT-PCR. Several genes in the integrin family were upregulated in the group that responded well to ES compared with the group that responded poorly. Through haplotype analysis, we found that breed and parent of origin KDGDQHIIHFWRQWHQGHUQHVV%UHHGRIRULJLQRILQWHJULQDOSKDITGA6 FRUUHVSRQGHGWRDNJGLIIHUHQFHLQ(6UHVLGXDOWHQGHUQHVVYDOXHV ZKHQLQKHULWHGPDWHUQDOO\P 7KHJHQH¿EURQHFWLQFN1 had DGLIIHUHQFHLQ(6UHVLGXDOWHQGHUQHVVRINJIRUGLIIHUHQWSDWHUQDOO\ LQKHULWHGKDSORW\SHVP$OVRITGA6 SURWHLQH[SUHVVLRQZDV FORVHO\UHODWHGWRP51$H[SUHVVLRQLQWKHVXEVHWDQDO\]HGE\:HVWHUQ EORWJHQHDQGSURWHLQH[SUHVVLRQOHYHOVZHUHERWKIROGKLJKHULQ the tender group than the low; P = 0.04 and P = UHVSHFWLYHO\7KLV DSSURDFKLGHQWL¿HGDQHWZRUNDQGELRORJLFDOPHFKDQLVPDVVRFLDWHGZLWK tenderness not previously established. These results also suggest that WDUJHWLQJFRPSRQHQWVRIWKH(&0UHSUHVHQWVDQRYHODUHDRIUHVHDUFK for improving tenderness.
Key Words: tenderness, beef, electrical stimulation
LB7 A new inline device for predicting individual cow somatic cell count using ATP measurement technology.%::RRGZDUG 1,
$-6H\NRUD2, and T. Koopman3, 1NextGen, Lawrenceville, GA, 2
Uni-versity of Minnesota, St. Paul, 3Isogen Animal Care, De Meern, the