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Validating the use of an ultra-performance liquid chromatography with tandem mass spectrometry method to quantify equol in cow's milk

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Academic year: 2021

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Fig. 1 Optimized elution gradient (dotted line) and TIC of equol and daidzein-d 4 (IS) set at 50 ng.mL -1 and 100 ng.mL -1 , respectively
Table 1: Estimation of precision and accuracy of the UPLC®-MS/MS method, process efficiency, and assessment of sample extract stability after a 7-day storage at 5±1 °C and -21± 1°C
Fig. 2 Robustness evaluation of the enzymatic hydrolysis process (n≥3). Bars with the same letters (a, b, and c) are not significantly different (P value>0.05)
Fig. 3 Equol content found in Belgian commercial cow's milk and raw milk analyzed by UPLC ® -MS/MS

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