Implication des prostaglandines dans l'accumulation d'OPG dans le surnageant d'ostéoblastes et dans un modèle d'ostéoclastogénèse

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Figure 11: Effect of exogenous PGE2 on the accumulation of OPG in the supematants of MG-63, Saos-2 and hOB in the presence of COX inhibitors.

PGEj (10"^ M)

significantly decreased the accumulation of OPG in the

supematants of hOB but not in immortalized cell Unes. ★ P< 0.05 compared to control at the same time point. N = 3-5 (in immortalized cell lines, N is the number of différent experiments and in human osteoblasts represent the number of différent donors used to obtain osteoblasts).

Indomethacin and Diclofenac were used at the concentrations of 10"® M and 10'® M respectively.

A) Human osteoblasts in the presence of IND B) Human osteoblasts in the presence of DIC C) MG-63 cells in the presence of IND D) MG-63 cells in the presence of DIC E) Saos-2 cell line in the presence of IND F) Saos-2 cell line in the presence of DIC

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Figure 20: Effect of U46619 an agonist of TP receptor on the accumulation of OPG in the supematants of MG-63, Saos-2 and hOB in the presence of COX inhibitors. The TP (10"^ M) agonist had no effect on the accumulation of OPG. N = 3-5 (in immortalized cell lines, N is the number of différent experiments and in human osteohlasts it represents the number of différent donors used to ohtain osteohlasts). Indomethacin and Diclofenac were used

at the concentrations of 10"^ M

and 10'^ M

respectively.

A) Human osteohlasts in the presence of IND B) Human osteohlasts in the presence of DIC G) MG-63 cells in the presence of IND D) MG-63 cells in the presence of DIC E) Saos-2 cell line in the presence of IND F) Saos-2 cell line in the presence of DIC

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