Identification of critical cell concentrations with in-situ and ex-situ characterization of physico-chemical properties of broth during oxidative axenic cultures of Yarrowia lipolytica in fed-batch mode

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This is an author-deposited version published in : http://oatao.univ-toulouse.fr/ Eprints ID : 10298

To cite this version : Kraiem, Hazar and Manon, Yannick and Anne-Archard,

Dominique and Fillaudeau, Luc. Identification of critical cell concentrations with in-situ and ex-situ

characterization of physico-chemical properties of broth during oxidative axenic cultures of

Yarrowia lipolytica in fed-batch mode. (2012) In: BioProtech International Conference 2012 - BICB

2012, 05 November 2012 - 08 November 2012 (Sfax, Tunisia). (Unpublished)

Any correspondance concerning this service should be sent to the repository administrator: staff-oatao@listes-diff.inp-toulouse.fr

Bioprotech International Conference on Bioproduction BICB 2012

Sfax, Tunisia, 05-08 November 2012.

Identification of critical cell concentrations with in-situ and ex-situ

characterization of physico-chemical properties of broth during oxidative axenic

cultures of Y arrowia lipolytica in fed-batch mode.

Hazar KRAIEMS, Y annick MANONa,b, Dominique ANNE-ARCHARDb and Luc FILLAUDEAU~

a Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés, CNRS UMR5504, INRA UMR 792, lNSA,

Université de Toulouse, 135, avenue de Rangueil F-31077 Toulouse, France

b Université de Toulouse; lNPT, UPS; IMFT (Institut de Mécanique des Fluides de Toulouse), Toulouse, France

Mots-clés: Broth, cell culture, fed-batch, on-line and off-line measurements, physical and

physicochemical properties, rheometry, Yarrowia lipolytica.

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Fig]: Evolution of respiratory coefficient and OUR as a function of cel! concentration (Cultures #1, 2

and 3) -Illustration of mycelian transition

-Fig2. ln-situ apparent viscosity as a function of cel! concentration in Dl and D2 ducts-ldentification a critical cell concentration (Cultures #1, 2 and 3) .