Soil microbial community composition changes according to
the tillage practice and plant development stage
Agricultural practices have a strong impact on
soil bacterial and fungal community composition
. Furthermore, microbial community composition can change with the stage of plantdevelopment. We are interested in exploring these effects in relation to changes induced by
agriculture
(conventional and reduced tillage) andplant stage
(germination and flowering) in soil conditions. Here, instead of examining this impact at a high taxonomic level such as phylum and/or class, thus missing potentially relevant information from lower levels, we propose anoriginal
method
: exploiting the available sequence information at the lowest taxonomic level attainable for each operational taxonomic unit.C
ONTEXT
(1) Soilsampling
on Faba bean agricultural fieldM
ETHOD
1
2
(4) Sequencesanalysis using the
mothur
pipeline (5) Microbial compositon analysis usingordination
methods (2)DNA
extraction (3) Massive sequencing using454
technologyR
ESULTS
Their abundance are higher at
flowering
stage.Their abundance are higher under
conventional
tillage.
3
B
ACTERIA
F
UNGI
Their abundance are higher atflowering
stage.
Their abundance are
higher at
germination
stage.F. Degrune
12, M. Dufrêne
3, B. Taminiau
4, S. Barbieux
15, M-P. Hiel
16, G. Daube
4, C. Nezer
7, M. Vandenbol
21AgricultureIsLife, 2Unité de Microbiologie et Génomique, 6Unité de Phytotechnie des Régions Tempérées et Ferme expérimentale, 3Unité Biodiversité et Paysage, and 5Unité Système
Sol-Eau (Gembloux Agro-Bio tech, Ulg , Belgium), 4 Unité de Microbiologie des Aliments, Faculté de médecine vétérinaire (ULg, Belgium), 7Quality Partner s.a. (Herstal, Belgium)